Restriction map for
pF1KSDA1869
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoT22I FbaI FspI HincII
HindII HpaI KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 2058 TCCGGA T^CCGGA
Asp700I 423 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 700 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1111 GGATCC G^GATCC
BbeI 1849 GGCGCC GGCGC^C
BbsI 952 GTCTTC GAAGAC(2/6)
BbvCI 2451 GCTGAGG CCTCAGC(-5/-2)
BcgI 1910 GCAAGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 952 GTCTTC GAAGAC(2/6)
BsaBI 1663 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2058 TCCGGA T^CCGGA
BsiEI 2775 CGGCCG CG[GA][CT]^CG
BspEI 2058 TCCGGA T^CCGGA
BssSI 2434 CACGAG CACGAG(-5/-1)
BstAPI 1834 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1071 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2775 CGGCCG C^GGCCG
EclXI 2775 CGGCCG C^GGCCG
Eco52I 2775 CGGCCG C^GGCCG
EcoRV 1639 GATATC GAT^ATC
FseI 2659 GGCCGGCC GGCCGG^CC
HindIII 844 AAGCTT A^AGCTT
KasI 1849 GGCGCC G^GCGCC
MamI 1663 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2058 TCCGGA T^CCGGA
MseI 2368 TTAA T^TAA
MslI 1314 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2660 GCCGGC GCC^GGC
NarI 1849 GGCGCC GG^CGCC
NcoI 1691 CCATGG C^CATGG
NgoMIV 2660 GCCGGC G^CCGGC
PaeR7I 2594 CTCGAG C^TCGAG
PflFI 700 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 569 CAGCTG CAG^CTG
SfiI 1821 GGCCAGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1849 GGCGCC GGC^GCC
SmlI 2594 CTCGAG C^T[CT][GA]AG
Tru9I 2368 TTAA T^TAA
Tth111I 700 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2594 CTCGAG C^TCGAG
XmnI 423 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AatII 1901 GACGTC GACGT^C
2701 GACGTC
AccI 706 GTCTAC GT^[AC][GT]AC
1534 GTCTAC
AlwNI 1626 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1856 CAGTCTCTG
BalI 533 TGGCCA TGG^CCA
1820 TGGCCA
BanI 285 GGTGCC G^G[CT][GA]CC
1849 GGCGCC
BfaI 220 CTAG C^TAG
1253 CTAG
BglI 1822 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2448 GCCGCTGAGGC
BsaWI 209 ACCGGA [AT]^CCGG[AT]
2058 TCCGGA
BstXI 127 CCATTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1394 CCATCCTCCTGG
BstYI 1111 GGATCC [GA]^GATC[CT]
1648 GGATCT
EarI 1149 CTCTTC CTCTTC(1/4)
1405 GAAGAG
EcoNI 1097 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1571 CCTGTCAGAGG
HaeII 1849 GGCGCC [GA]GCGC^[CT]
2379 GGCGCT
Ksp632I 1149 CTCTTC CTCTTC(1/4)
1405 GAAGAG
MaeI 220 CTAG C^TAG
1253 CTAG
MflI 1111 GGATCC [GA]^GATC[CT]
1648 GGATCT
MluNI 533 TGGCCA TGG^CCA
1820 TGGCCA
MscI 533 TGGCCA TGG^CCA
1820 TGGCCA
PstI 167 CTGCAG CTGCA^G
466 CTGCAG
StuI 683 AGGCCT AGG^CCT
902 AGGCCT
XcmI 1603 CCATACTGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1625 CCAGGGGCTGTGTGG
XhoII 1111 GGATCC [GA]^GATC[CT]
1648 GGATCT
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XmaCI XmaI