Restriction map for
pF1KSDA1895
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccII AccIII AciI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstYI CelII Cfr10I ClaI CpoI
DraII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MflI MluI MroI MspA1I MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1542 GTATAC GT^[AC][GT]AC
AclI 996 AACGTT AA^CGTT
AflII 280 CTTAAG C^TTAAG
AflIII 1497 ACGTGT A^C[GA][CT]GT
AlwI 1461 GATCC GGATC(4/5)
AocI 1465 CCTCAGG CC^T[ACGT]AGG
Asp700I 1495 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GAGCAC G[AT]GC[AT]^C
AvaII 816 GGTCC G^G[AT]CC
BbsI 202 GAAGAC GAAGAC(2/6)
BfaI 134 CTAG C^TAG
BfrI 280 CTTAAG C^TTAAG
BmyI 64 GAGCAC G[AGT]GC[ACT]^C
BpuAI 202 GAAGAC GAAGAC(2/6)
BsaWI 78 TCCGGT [AT]^CCGG[AT]
BseRI 137 GAGGAG GAGGAG(10/8)
BsgI 824 GTGCAG GTGCAG(16/14)
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
BsmI 422 GAATGC GAATGC(1/-1)
Bsp1286I 64 GAGCAC G[AGT]GC[ACT]^C
BspMI 1111 ACCTGC ACCTGC(4/8)
Bst1107I 1542 GTATAC GTA^TAC
BstZ17I 1542 GTATAC GTA^TAC
Bsu36I 1465 CCTCAGG CC^T[ACGT]AGG
CfoI 624 GCGC GCG^C
Cfr13I 816 GGTCC G^G[ACGT]CC
DpnII 1461 GATC ^GATC
DraI 1123 TTTAAA TTT^AAA
DrdI 1315 GACTATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1465 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1663 ATGCAT ATGCA^T
HhaI 624 GCGC GCG^C
HinP1I 624 GCGC G^CGC
HindIII 607 AAGCTT A^AGCTT
MaeI 134 CTAG C^TAG
MboI 1461 GATC ^GATC
MfeI 563 CAATTG C^AATTG
MunI 563 CAATTG C^AATTG
NdeII 1461 GATC ^GATC
NsiI 1663 ATGCAT ATGCA^T
PflMI 1390 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 996 AACGTT AA^CGTT
Sau3AI 1461 GATC ^GATC
Sau96I 816 GGTCC G^G[ACGT]CC
ScaI 109 AGTACT AGT^ACT
SmlI 280 CTTAAG C^T[CT][GA]AG
SphI 1665 GCATGC GCATG^C
Van91I 1390 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 133 TCTAGA T^CTAGA
XmnI 1495 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BpmI 331 CTCCAG CTGGAG(16/14)
703 CTGGAG
BsaI 140 GAGACC GGTCTC(1/5)
81 GGTCTC
BsmBI 1054 CGTCTC CGTCTC(1/5)
1175 GAGACG
BsmFI 1085 GGGAC GGGAC(10/14)
817 GTCCC
DraIII 290 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
1391 CACTTAGTG
DsaI 1730 CCATGG C^C[GA][CT]GG
1771 CCATGG
EarI 1243 GAAGAG CTCTTC(1/4)
1306 GAAGAG
HapII 79 CCGG C^CGG
1622 CCGG
HpaII 79 CCGG C^CGG
1622 CCGG
Ksp632I 1243 GAAGAG CTCTTC(1/4)
1306 GAAGAG
MslI 864 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1420 CATCCATATG
MspI 79 CCGG C^CGG
1622 CCGG
NcoI 1730 CCATGG C^CATGG
1771 CCATGG
NdeI 1424 CATATG CA^TATG
1537 CATATG
NlaIV 816 GGTCCC GG[ACGT]^[ACGT]CC
1624 GGCTCC
NspI 1152 GCATGT [GA]CATG^[CT]
1665 GCATGC
SapI 1243 GAAGAGC GCTCTTC(1/4)
1306 GAAGAGC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI ApoI BalI
BbvI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstXI
Cac8I DdeI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboII MluNI
MnlI MscI MseI MvaI MwoI
NlaIII PleI RsaI ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I