Restriction map for
pF1KSDA1904
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# NO CUT ENZYMES ( 90 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbrPI BciVI
BclI BfaI BfrI BlnI BsaWI
BseAI BsiWI BsmBI BsmI BspEI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MaeI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SexAI SfiI SfuI
SnaBI SpeI SspI StuI SwaI
TfiI Tsp509I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AatII 1003 GACGTC GACGT^C
AflIII 98 ACGTGT A^C[GA][CT]GT
Aor51HI 2312 AGCGCT AGC^GCT
ApaI 1781 GGGCCC GGGCC^C
BalI 107 TGGCCA TGG^CCA
BsaAI 97 TACGTG [CT]AC^GT[GA]
BsaBI 963 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1654 ATCGAT AT^CGAT
BsrDI 1608 CATTGC GCAATG(2/0)
BssSI 973 CTCGTG CACGAG(-5/-1)
ClaI 1654 ATCGAT AT^CGAT
EarI 577 CTCTTC CTCTTC(1/4)
Eco47III 2312 AGCGCT AGC^GCT
Ksp632I 577 CTCTTC CTCTTC(1/4)
KspI 652 CCGCGG CCGC^GG
MamI 963 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 107 TGGCCA TGG^CCA
MscI 107 TGGCCA TGG^CCA
MseI 1643 TTAA T^TAA
PaeR7I 1570 CTCGAG C^TCGAG
SacI 1824 GAGCTC GAGCT^C
SacII 652 CCGCGG CCGC^GG
SbfI 312 CCTGCAGG CCTGCA^GG
ScaI 2279 AGTACT AGT^ACT
SgrAI 1524 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1777 CCCGGG CCC^GGG
Sse8387I 312 CCTGCAGG CCTGCA^GG
Tru9I 1643 TTAA T^TAA
XhoI 1570 CTCGAG C^TCGAG
XmaCI 1777 CCCGGG C^CCGGG
XmaI 1777 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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Alw44I 169 GTGCAC G^TGCAC
1357 GTGCAC
AlwI 837 GATCC GGATC(4/5)
2406 GGATC
ApaLI 169 GTGCAC G^TGCAC
1357 GTGCAC
AspI 1141 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1624 GACAAGGTC
BbeI 1161 GGCGCC GGCGC^C
1522 GGCGCC
BbsI 1305 GAAGAC GAAGAC(2/6)
604 GTCTTC
BbvCI 486 CCTCAGC CCTCAGC(-5/-2)
1763 CCTCAGC
BlpI 342 GCTCAGC GC^T[ACGT]AGC
1851 GCTGAGC
Bpu1102I 342 GCTCAGC GC^T[ACGT]AGC
1851 GCTGAGC
BpuAI 1305 GAAGAC GAAGAC(2/6)
604 GTCTTC
BsaI 139 GAGACC GGTCTC(1/5)
2439 GGTCTC
BsgI 901 CTGCAC GTGCAG(16/14)
976 GTGCAG
Bsp1407I 965 TGTACA T^GTACA
2351 TGTACA
BspHI 1007 TCATGA T^CATGA
1193 TCATGA
BsrBI 2205 CCGCTC CCGCTC(-3/-3)
1789 GAGCGG
BsrGI 965 TGTACA T^GTACA
2351 TGTACA
CelII 342 GCTCAGC GC^T[ACGT]AGC
1851 GCTGAGC
DsaI 652 CCGCGG C^C[GA][CT]GG
869 CCACGG
EcoNI 351 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
411 CCTCATCGAGG
HincII 1294 GTCAAC GT[CT]^[GA]AC
1630 GTCAAC
HindII 1294 GTCAAC GT[CT]^[GA]AC
1630 GTCAAC
KasI 1161 GGCGCC G^GCGCC
1522 GGCGCC
NarI 1161 GGCGCC GG^CGCC
1522 GGCGCC
NspI 362 GCATGC [GA]CATG^[CT]
1268 GCATGC
PflFI 1141 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1624 GACAAGGTC
PflMI 1368 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2082 CCACCACCTGG
RcaI 1007 TCATGA T^CATGA
1193 TCATGA
SfoI 1161 GGCGCC GGC^GCC
1522 GGCGCC
SphI 362 GCATGC GCATG^C
1268 GCATGC
SspBI 965 TGTACA T^GTACA
2351 TGTACA
Tth111I 1141 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1624 GACAAGGTC
Van91I 1368 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2082 CCACCACCTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII AviII
BanI BanII BbvI BcgI BcnI
BglI BglII BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII