Restriction map for
pF1KSDA1942
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccIII AclI AcsI AfaI
AflII AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbsI BciVI
BclI BfaI BfrI BglI BlnI
BpuAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 928 GACGTC GACGT^C
AccI 94 GTCTAC GT^[AC][GT]AC
AflIII 28 ACGTGT A^C[GA][CT]GT
Alw44I 1228 GTGCAC G^TGCAC
AlwI 219 GGATC GGATC(4/5)
AocI 1165 CCTGAGG CC^T[ACGT]AGG
ApaLI 1228 GTGCAC G^TGCAC
AspI 208 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 187 GGCGCC GGCGC^C
BbvCI 415 CCTCAGC CCTCAGC(-5/-2)
BcgI 645 CGAGGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 998 AGATCT A^GATCT
BlpI 839 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 839 GCTCAGC GC^T[ACGT]AGC
BsaI 1240 GAGACC GGTCTC(1/5)
BssHII 7 GCGCGC G^CGCGC
BstXI 441 CCACTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 998 AGATCT [GA]^GATC[CT]
Bsu36I 1165 CCTGAGG CC^T[ACGT]AGG
CelII 839 GCTCAGC GC^T[ACGT]AGC
DraIII 1134 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 105 CGGCCG C^GGCCG
EclXI 105 CGGCCG C^GGCCG
Eco52I 105 CGGCCG C^GGCCG
Eco81I 1165 CCTGAGG CC^T[ACGT]AGG
HinfI 58 GAGTC G^A[ACGT]TC
KasI 187 GGCGCC G^GCGCC
MflI 998 AGATCT [GA]^GATC[CT]
MslI 442 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 187 GGCGCC GG^CGCC
NspI 896 GCATGC [GA]CATG^[CT]
PflFI 208 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 570 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 58 GAGTC GAGTC(4/5)
PshAI 1068 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 187 GGCGCC GGC^GCC
SmaI 671 CCCGGG CCC^GGG
SmlI 994 CTCAAG C^T[CT][GA]AG
SphI 896 GCATGC GCATG^C
TaqI 1076 TCGA T^CGA
Tth111I 208 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1076 TCGA T^CGA
Van91I 570 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 998 AGATCT [GA]^GATC[CT]
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 8 CGCG CG^CG
124 CGCG
AgeI 679 ACCGGT A^CCGGT
694 ACCGGT
ApaI 81 GGGCCC GGGCC^C
989 GGGCCC
AspHI 163 GTGCTC G[AT]GC[AT]^C
1228 GTGCAC
AvaI 671 CCCGGG C^[CT]CG[GA]G
1113 CTCGGG
BalI 428 TGGCCA TGG^CCA
1037 TGGCCA
BpmI 421 CTGGAG CTGGAG(16/14)
945 CTGGAG
BsgI 1258 CTGCAC GTGCAG(16/14)
1118 GTGCAG
BsiEI 105 CGGCCG CG[GA][CT]^CG
681 CGGTCG
BsiHKAI 163 GTGCTC G[AT]GC[AT]^C
1228 GTGCAC
BsoBI 671 CCCGGG C^[CT]CG[GA]G
1113 CTCGGG
BsrBI 406 GAGCGG CCGCTC(-3/-3)
1156 GAGCGG
BsrFI 679 ACCGGT [GA]^CCGG[CT]
694 ACCGGT
BstEII 675 GGTGACC G^GT[ACGT]ACC
806 GGTCACC
BstPI 675 GGTGACC G^GT[ACGT]ACC
806 GGTCACC
BstUI 8 CGCG CG^CG
124 CGCG
Cfr10I 679 ACCGGT [GA]^CCGG[CT]
694 ACCGGT
DrdI 928 GACGTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1093 GACAGCGGGGTC
DsaI 47 CCATGG C^C[GA][CT]GG
431 CCATGG
EcoO65I 675 GGTGACC G^GT[ACGT]ACC
806 GGTCACC
EcoT14I 47 CCATGG C^C[AT][AT]GG
431 CCATGG
MluNI 428 TGGCCA TGG^CCA
1037 TGGCCA
MscI 428 TGGCCA TGG^CCA
1037 TGGCCA
MvnI 8 CGCG CG^CG
124 CGCG
NcoI 47 CCATGG C^CATGG
431 CCATGG
PinAI 679 ACCGGT A^CCGGT
694 ACCGGT
PstI 550 CTGCAG CTGCA^G
799 CTGCAG
SapI 403 GAAGAGC GCTCTTC(1/4)
484 GAAGAGC
SfcI 550 CTGCAG C^T[GA][CT]AG
799 CTGCAG
StuI 155 AGGCCT AGG^CCT
890 AGGCCT
StyI 47 CCATGG C^C[AT][AT]GG
431 CCATGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaII
BanI BanII BbrPI BbvI BcnI
BmyI BsaAI BsaHI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PmaCI
PmlI PpuMI PvuII Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI