Restriction map for
pF1KSDA1949
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII AccII AccIII AclI AcsI
AflIII AgeI AhdI AlwNI Aor51HI
ApoI AscI AseI AsnI AspEI
AvrII BbrPI BbsI BcgI BciVI
BclI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KspI MaeIII
MamI MfeI MluI MroI MunI
MvnI NcoI NdeI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tsp509I
XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 1514 GGTACC G^GTACC
AccI 662 GTAGAC GT^[AC][GT]AC
AflII 1612 CTTAAG C^TTAAG
Alw44I 253 GTGCAC G^TGCAC
ApaLI 253 GTGCAC G^TGCAC
Asp700I 261 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1514 GGTACC G^GTACC
AspI 428 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1799 TGCGCA TGC^GCA
BamHI 1335 GGATCC G^GATCC
BbeI 41 GGCGCC GGCGC^C
BbvCI 786 GCTGAGG CCTCAGC(-5/-2)
BfrI 1612 CTTAAG C^TTAAG
BsaAI 1647 CACGTA [CT]AC^GT[GA]
BsmBI 1558 CGTCTC CGTCTC(1/5)
BspHI 1343 TCATGA T^CATGA
BstXI 1323 CCAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FspI 1799 TGCGCA TGC^GCA
HincII 1420 GTCAAC GT[CT]^[GA]AC
HindII 1420 GTCAAC GT[CT]^[GA]AC
KasI 41 GGCGCC G^GCGCC
KpnI 1514 GGTACC GGTAC^C
MaeII 1648 ACGT A^CGT
MseI 1613 TTAA T^TAA
MslI 566 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 41 GGCGCC GG^CGCC
PflFI 428 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 690 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1343 TCATGA T^CATGA
SfoI 41 GGCGCC GGC^GCC
Tru9I 1613 TTAA T^TAA
Tth111I 428 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 690 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 982 CCAACAGAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 261 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 41 GGCGCC G[GA]^CG[CT]C
57 GGCGTC
AocI 489 CCTGAGG CC^T[ACGT]AGG
1699 CCTGAGG
ApaI 1388 GGGCCC GGGCC^C
1689 GGGCCC
BalI 2 TGGCCA TGG^CCA
844 TGGCCA
BanI 41 GGCGCC G^G[CT][GA]CC
1514 GGTACC
BglII 1532 AGATCT A^GATCT
1616 AGATCT
BsaHI 41 GGCGCC G[GA]^CG[CT]C
57 GGCGTC
BsrFI 44 GCCGGC [GA]^CCGG[CT]
1832 GCCGGC
Bsu36I 489 CCTGAGG CC^T[ACGT]AGG
1699 CCTGAGG
Cfr10I 44 GCCGGC [GA]^CCGG[CT]
1832 GCCGGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
844 TGGCCA
Eco81I 489 CCTGAGG CC^T[ACGT]AGG
1699 CCTGAGG
HgaI 1219 GACGC GACGC(5/10)
58 GCGTC
Hin1I 41 GGCGCC G[GA]^CG[CT]C
57 GGCGTC
HindIII 148 AAGCTT A^AGCTT
1090 AAGCTT
HphI 1365 GGTGA GGTGA(8/7)
1415 TCACC
MluNI 2 TGGCCA TGG^CCA
844 TGGCCA
MscI 2 TGGCCA TGG^CCA
844 TGGCCA
MspA1I 28 CAGCTG C[AC]G^C[GT]G
1569 CAGCTG
NaeI 44 GCCGGC GCC^GGC
1832 GCCGGC
NgoMIV 44 GCCGGC G^CCGGC
1832 GCCGGC
NheI 33 GCTAGC G^CTAGC
324 GCTAGC
PaeR7I 626 CTCGAG C^TCGAG
1022 CTCGAG
PpuMI 1585 GGGTCCC [GA]G^G[AT]CC[CT]
1703 AGGTCCC
PvuII 28 CAGCTG CAG^CTG
1569 CAGCTG
SapI 316 GAAGAGC GCTCTTC(1/4)
1759 GAAGAGC
SmaI 356 CCCGGG CCC^GGG
1494 CCCGGG
XhoI 626 CTCGAG C^TCGAG
1022 CTCGAG
XmaCI 356 CCCGGG C^CCGGG
1494 CCCGGG
XmaI 356 CCCGGG C^CCGGG
1494 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI TspRI
TthHB8I XhoII