Restriction map for
pF1KSDA1956
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FokI FseI HaeII
HapII Hin1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XcmI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 264 CGCG CG^CG
AciI 376 CCGC CCGC(-3/-1)
AcsI 1717 AAATTC [GA]^AATT[CT]
AflIII 645 ACACGT A^C[GA][CT]GT
AlwI 145 GGATC GGATC(4/5)
AlwNI 319 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1236 CCTTAGG CC^T[ACGT]AGG
ApoI 1717 AAATTC [GA]^AATT[CT]
AspHI 1647 GAGCTC G[AT]GC[AT]^C
AvaI 1841 CTCGAG C^[CT]CG[GA]G
AviII 1812 TGCGCA TGC^GCA
BanII 1647 GAGCTC G[GA]GC[CT]^C
BsaAI 646 CACGTA [CT]AC^GT[GA]
BsiHKAI 1647 GAGCTC G[AT]GC[AT]^C
BsoBI 1841 CTCGAG C^[CT]CG[GA]G
BspMI 220 GCAGGT ACCTGC(4/8)
BstAPI 318 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 264 CGCG CG^CG
Bsu36I 1236 CCTTAGG CC^T[ACGT]AGG
DraI 1819 TTTAAA TTT^AAA
Eco81I 1236 CCTTAGG CC^T[ACGT]AGG
EcoT14I 393 CCTTGG C^C[AT][AT]GG
EcoT22I 621 ATGCAT ATGCA^T
FspI 1812 TGCGCA TGC^GCA
HgaI 731 GCGTC GACGC(5/10)
MspA1I 609 CAGCTG C[AC]G^C[GT]G
MvnI 264 CGCG CG^CG
MwoI 318 GCAGAAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 1442 CATATG CA^TATG
NsiI 621 ATGCAT ATGCA^T
PaeR7I 1841 CTCGAG C^TCGAG
PvuII 609 CAGCTG CAG^CTG
SacI 1647 GAGCTC GAGCT^C
SmlI 1841 CTCGAG C^T[CT][GA]AG
SpeI 1093 ACTAGT A^CTAGT
StyI 393 CCTTGG C^C[AT][AT]GG
TfiI 384 GAATC G^A[AT]TC
XbaI 676 TCTAGA T^CTAGA
XhoI 1841 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Asp700I 1716 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1973 GAAGCTCTTC
BanI 162 GGCACC G^G[CT][GA]CC
1147 GGCACC
BsmI 619 GAATGC GAATGC(1/-1)
1774 GAATGC
CfoI 263 GCGC GCG^C
1813 GCGC
EcoNI 63 CCTTAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
214 CCTGGGGCAGG
HhaI 263 GCGC GCG^C
1813 GCGC
HinP1I 263 GCGC G^CGC
1813 GCGC
MaeII 95 ACGT A^CGT
647 ACGT
MaeIII 1088 GTCAC ^GT[ACGT]AC
1256 GTCAC
MseI 989 TTAA T^TAA
1820 TTAA
PstI 526 CTGCAG CTGCA^G
1682 CTGCAG
Tru9I 989 TTAA T^TAA
1820 TTAA
Tsp45I 1088 GTCAC ^GT[GC]AC
1256 GTCAC
XmnI 1716 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1973 GAAGCTCTTC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI BbvI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HinfI ItaI
Ksp632I MaeI MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
PleI RsaI SapI Sau3AI ScrFI
SfaNI SfcI StuI TaqI TseI
Tsp509I TspRI TthHB8I