Restriction map for
pF1KSDA1957
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaI BsgI
BsiWI BsmBI BsmI BspDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HindIII HpaI
KpnI Ksp632I MaeI MaeIII MfeI
MluI MluNI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 435 GACGTC GACGT^C
AccIII 1154 TCCGGA T^CCGGA
AflIII 14 ACATGT A^C[GA][CT]GT
Alw44I 970 GTGCAC G^TGCAC
AlwNI 226 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1167 CCTCAGG CC^T[ACGT]AGG
ApaLI 970 GTGCAC G^TGCAC
BbsI 1108 GTCTTC GAAGAC(2/6)
BcgI 1076 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1108 GTCTTC GAAGAC(2/6)
BsaBI 333 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1154 TCCGGA [AT]^CCGG[AT]
BseAI 1154 TCCGGA T^CCGGA
BseRI 367 GAGGAG GAGGAG(10/8)
Bsp1407I 110 TGTACA T^GTACA
BspEI 1154 TCCGGA T^CCGGA
BspHI 611 TCATGA T^CATGA
BspLU11I 14 ACATGT A^CATGT
BspMI 974 ACCTGC ACCTGC(4/8)
BsrDI 116 GCAATG GCAATG(2/0)
BsrGI 110 TGTACA T^GTACA
BssSI 474 CACGAG CACGAG(-5/-1)
BstAPI 1022 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 153 CATCC GGATG(2/0)
BstXI 879 CCAGGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 137 AGATCC [GA]^GATC[CT]
Bsu36I 1167 CCTCAGG CC^T[ACGT]AGG
DdeI 1168 CTCAG C^T[ACGT]AG
DraIII 763 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1167 CCTCAGG CC^T[ACGT]AGG
EcoNI 306 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 153 CATCC GGATG(9/13)
FseI 948 GGCCGGCC GGCCGG^CC
HincII 123 GTTGAC GT[CT]^[GA]AC
HindII 123 GTTGAC GT[CT]^[GA]AC
KspI 179 CCGCGG CCGC^GG
MamI 333 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 137 AGATCC [GA]^GATC[CT]
MroI 1154 TCCGGA T^CCGGA
MslI 1080 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 14 ACATGT [GA]CATG^[CT]
PflMI 11 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1063 CTGCAG CTGCA^G
PvuII 557 CAGCTG CAG^CTG
RcaI 611 TCATGA T^CATGA
SacII 179 CCGCGG CCGC^GG
SfaNI 1022 GCATC GCATC(5/9)
SfcI 1063 CTGCAG C^T[GA][CT]AG
SmlI 1201 CTCAAG C^T[CT][GA]AG
SspBI 110 TGTACA T^GTACA
StuI 92 AGGCCT AGG^CCT
Van91I 11 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 137 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 111 GTAC GT^AC
942 GTAC
AhdI 355 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
649 GACACAGGGTC
AspEI 355 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
649 GACACAGGGTC
BbeI 545 GGCGCC GGCGC^C
830 GGCGCC
BbrPI 328 CACGTG CAC^GTG
904 CACGTG
BsaAI 328 CACGTG [CT]AC^GT[GA]
904 CACGTG
BsmAI 489 GTCTC GTCTC(1/5)
708 GTCTC
BsrBI 699 CCGCTC CCGCTC(-3/-3)
28 GAGCGG
BsrFI 827 GCCGGC [GA]^CCGG[CT]
949 GCCGGC
BsrI 356 ACTGG ACTGG(1/-1)
1161 ACTGG
Cfr10I 827 GCCGGC [GA]^CCGG[CT]
949 GCCGGC
EagI 621 CGGCCG C^GGCCG
662 CGGCCG
Eam1105I 355 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
649 GACACAGGGTC
EclXI 621 CGGCCG C^GGCCG
662 CGGCCG
Eco52I 621 CGGCCG C^GGCCG
662 CGGCCG
HaeII 545 GGCGCC [GA]GCGC^[CT]
830 GGCGCC
KasI 545 GGCGCC G^GCGCC
830 GGCGCC
MspA1I 179 CCGCGG C[AC]G^C[GT]G
557 CAGCTG
NaeI 827 GCCGGC GCC^GGC
949 GCCGGC
NarI 545 GGCGCC GG^CGCC
830 GGCGCC
NgoMIV 827 GCCGGC G^CCGGC
949 GCCGGC
PmaCI 328 CACGTG CAC^GTG
904 CACGTG
PmlI 328 CACGTG CAC^GTG
904 CACGTG
PpuMI 655 GGGTCCT [GA]G^G[AT]CC[CT]
682 GGGTCCC
RsaI 111 GTAC GT^AC
942 GTAC
SfiI 234 GGCCTCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
948 GGCCGGCCAGGCC
SfoI 545 GGCGCC GGC^GCC
830 GGCGCC
SmaI 62 CCCGGG CCC^GGG
820 CCCGGG
TaqI 341 TCGA T^CGA
1130 TCGA
TthHB8I 341 TCGA T^CGA
1130 TCGA
XmaCI 62 CCCGGG C^CCGGG
820 CCCGGG
XmaI 62 CCCGGG C^CCGGG
820 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BsaHI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TseI TspRI