Restriction map for
pF1KSDA1959
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BspEI BspHI
BsrDI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI FbaI FseI FspI
HgaI HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 703 GACGTC GACGT^C
AccII 157 CGCG CG^CG
AcyI 703 GACGTC G[GA]^CG[CT]C
AflIII 633 ACATGT A^C[GA][CT]GT
BanI 97 GGCACC G^G[CT][GA]CC
BbsI 535 GAAGAC GAAGAC(2/6)
BcgI 1305 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1541 GGATAC GTATCC(6/5)
BglI 1130 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 338 AGATCT A^GATCT
BpuAI 535 GAAGAC GAAGAC(2/6)
BsaHI 703 GACGTC G[GA]^CG[CT]C
BsaI 1930 GAGACC GGTCTC(1/5)
BsgI 1430 CTGCAC GTGCAG(16/14)
BsmBI 1075 GAGACG CGTCTC(1/5)
BsmI 955 GAATGC GAATGC(1/-1)
Bsp1407I 53 TGTACA T^GTACA
BspDI 1387 ATCGAT AT^CGAT
BspLU11I 633 ACATGT A^CATGT
BsrBI 25 CCGCTC CCGCTC(-3/-3)
BsrGI 53 TGTACA T^GTACA
BstAPI 580 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 232 GGTGACC G^GT[ACGT]ACC
BstPI 232 GGTGACC G^GT[ACGT]ACC
BstUI 157 CGCG CG^CG
ClaI 1387 ATCGAT AT^CGAT
EaeI 12 CGGCCA [CT]^GGCC[GA]
EcoO65I 232 GGTGACC G^GT[ACGT]ACC
EcoRV 748 GATATC GAT^ATC
EcoT22I 1335 ATGCAT ATGCA^T
Hin1I 703 GACGTC G[GA]^CG[CT]C
HincII 1123 GTCAAC GT[CT]^[GA]AC
HindII 1123 GTCAAC GT[CT]^[GA]AC
HindIII 307 AAGCTT A^AGCTT
MvnI 157 CGCG CG^CG
NsiI 1335 ATGCAT ATGCA^T
PpuMI 548 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 444 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1867 CAGCTG CAG^CTG
SacI 493 GAGCTC GAGCT^C
ScaI 1202 AGTACT AGT^ACT
SmlI 553 CTCAAG C^T[CT][GA]AG
SpeI 1350 ACTAGT A^CTAGT
SphI 1337 GCATGC GCATG^C
SspBI 53 TGTACA T^GTACA
TfiI 1630 GAATC G^A[AT]TC
XcmI 1899 CCATGGACCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbvCI 1276 CCTCAGC CCTCAGC(-5/-2)
1116 GCTGAGG
BspMI 1533 ACCTGC ACCTGC(4/8)
1750 ACCTGC
BsrFI 289 ACCGGC [GA]^CCGG[CT]
907 ACCGGC
Cfr10I 289 ACCGGC [GA]^CCGG[CT]
907 ACCGGC
DraII 548 AGGTCCT [GA]G^G[ACGT]CC[CT]
1763 AGGGCCT
EcoO109I 548 AGGTCCT [GA]G^G[ACGT]CC[CT]
1763 AGGGCCT
HaeII 89 AGCGCC [GA]GCGC^[CT]
117 GGCGCT
MseI 901 TTAA T^TAA
994 TTAA
NcoI 137 CCATGG C^CATGG
1899 CCATGG
PleI 920 GACTC GAGTC(4/5)
1080 GACTC
SmaI 260 CCCGGG CCC^GGG
829 CCCGGG
Tru9I 901 TTAA T^TAA
994 TTAA
XmaCI 260 CCCGGG C^CCGGG
829 CCCGGG
XmaI 260 CCCGGG C^CCGGG
829 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII