Restriction map for
pF1KSDA1969
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BbvI BcgI BclI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI ItaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI TseI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 443 CCGC CCGC(-3/-1)
AfaI 995 GTAC GT^AC
Alw44I 466 GTGCAC G^TGCAC
AlwI 1484 GATCC GGATC(4/5)
ApaLI 466 GTGCAC G^TGCAC
AspHI 466 GTGCAC G[AT]GC[AT]^C
AspI 244 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 274 GAGCCC G[GA]GC[CT]^C
BciVI 24 GTATCC GTATCC(6/5)
BlpI 163 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 163 GCTCAGC GC^T[ACGT]AGC
BseRI 139 GAGGAG GAGGAG(10/8)
BsgI 1331 CTGCAC GTGCAG(16/14)
BsiHKAI 466 GTGCAC G[AT]GC[AT]^C
BsiYI 887 CCTTCAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 887 CCTTCAACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1317 GTCCC GGGAC(10/14)
BsmI 148 GAATGC GAATGC(1/-1)
BspDI 1061 ATCGAT AT^CGAT
Cac8I 641 GCATGC GC[ACGT]^[ACGT]GC
CelII 163 GCTCAGC GC^T[ACGT]AGC
ClaI 1061 ATCGAT AT^CGAT
DpnII 1484 GATC ^GATC
EarI 288 GAAGAG CTCTTC(1/4)
HincII 659 GTCAAC GT[CT]^[GA]AC
HindII 659 GTCAAC GT[CT]^[GA]AC
HphI 251 TCACC GGTGA(8/7)
Ksp632I 288 GAAGAG CTCTTC(1/4)
MboI 1484 GATC ^GATC
MslI 860 CATATAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 1484 GATC ^GATC
NspI 641 GCATGC [GA]CATG^[CT]
PflFI 244 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1231 GAGTC GAGTC(4/5)
RsaI 995 GTAC GT^AC
Sau3AI 1484 GATC ^GATC
SexAI 209 ACCTGGT A^CC[AT]GGT
SfaNI 47 GATGC GCATC(5/9)
SmlI 31 CTCAAG C^T[CT][GA]AG
SphI 641 GCATGC GCATG^C
SspI 522 AATATT AAT^ATT
TaqI 1062 TCGA T^CGA
Tth111I 244 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1062 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AgeI 893 ACCGGT A^CCGGT
1313 ACCGGT
Asp700I 603 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
771 GAAACCCTTC
BalI 119 TGGCCA TGG^CCA
349 TGGCCA
BbsI 214 GTCTTC GAAGAC(2/6)
981 GTCTTC
BmyI 274 GAGCCC G[AGT]GC[ACT]^C
466 GTGCAC
BpuAI 214 GTCTTC GAAGAC(2/6)
981 GTCTTC
BsaWI 893 ACCGGT [AT]^CCGG[AT]
1313 ACCGGT
Bsp1286I 274 GAGCCC G[AGT]GC[ACT]^C
466 GTGCAC
BsrFI 893 ACCGGT [GA]^CCGG[CT]
1313 ACCGGT
Cfr10I 893 ACCGGT [GA]^CCGG[CT]
1313 ACCGGT
DraI 564 TTTAAA TTT^AAA
721 TTTAAA
EaeI 119 TGGCCA [CT]^GGCC[GA]
349 TGGCCA
HaeIII 120 GGCC GG^CC
350 GGCC
HapII 894 CCGG C^CGG
1314 CCGG
HpaII 894 CCGG C^CGG
1314 CCGG
MluNI 119 TGGCCA TGG^CCA
349 TGGCCA
MscI 119 TGGCCA TGG^CCA
349 TGGCCA
MspI 894 CCGG C^CGG
1314 CCGG
NlaIV 439 GGCTCC GG[ACGT]^[ACGT]CC
1316 GGTCCC
PinAI 893 ACCGGT A^CCGGT
1313 ACCGGT
TspRI 496 CAGTG CA[GC]TG[ACGT][ACGT]^
1434 CACTG
XmnI 603 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
771 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI ApoI AvaII BfaI
BpmI BsaJI BsmAI BsrI BssKI
BstF5I BstNI Cfr13I DdeI EcoRI
EcoRII FokI HindIII HinfI MaeI
MaeIII MboII MnlI MseI MvaI
NlaIII Sau96I ScrFI TfiI Tru9I
Tsp45I Tsp509I