Restriction map for
pF1KSDA1970
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BbvCI BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI BstXI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco52I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 1057 GAATTC [GA]^AATT[CT]
AhdI 1243 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 387 AGCGCT AGC^GCT
ApaI 337 GGGCCC GGGCC^C
ApoI 1057 GAATTC [GA]^AATT[CT]
AspEI 1243 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1106 GGCGCC GGCGC^C
BbrPI 727 CACGTG CAC^GTG
BbsI 396 GAAGAC GAAGAC(2/6)
BclI 989 TGATCA T^GATCA
BglI 131 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1363 AGATCT A^GATCT
BpuAI 396 GAAGAC GAAGAC(2/6)
BsaI 644 GAGACC GGTCTC(1/5)
BsaWI 400 ACCGGA [AT]^CCGG[AT]
BsmBI 753 CGTCTC CGTCTC(1/5)
Bsp1407I 173 TGTACA T^GTACA
BspHI 656 TCATGA T^CATGA
BsrDI 1091 GCAATG GCAATG(2/0)
BsrGI 173 TGTACA T^GTACA
Bst1107I 1258 GTATAC GTA^TAC
BstAPI 576 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1014 GGTCACC G^GT[ACGT]ACC
BstPI 1014 GGTCACC G^GT[ACGT]ACC
BstZ17I 1258 GTATAC GTA^TAC
Eam1105I 1243 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 660 GAAGAG CTCTTC(1/4)
Eco47III 387 AGCGCT AGC^GCT
EcoO65I 1014 GGTCACC G^GT[ACGT]ACC
EcoRI 1057 GAATTC G^AATTC
FbaI 989 TGATCA T^GATCA
KasI 1106 GGCGCC G^GCGCC
Ksp632I 660 GAAGAG CTCTTC(1/4)
MseI 1378 TTAA T^TAA
MslI 148 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1106 GGCGCC GG^CGCC
NspI 278 ACATGC [GA]CATG^[CT]
PmaCI 727 CACGTG CAC^GTG
PmlI 727 CACGTG CAC^GTG
PshAI 1008 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 656 TCATGA T^CATGA
SacI 442 GAGCTC GAGCT^C
SapI 660 GAAGAGC GCTCTTC(1/4)
SbfI 1236 CCTGCAGG CCTGCA^GG
SexAI 599 ACCTGGT A^CC[AT]GGT
SfoI 1106 GGCGCC GGC^GCC
Sse8387I 1236 CCTGCAGG CCTGCA^GG
SspBI 173 TGTACA T^GTACA
SspI 268 AATATT AAT^ATT
TaqI 1293 TCGA T^CGA
TfiI 913 GATTC G^A[AT]TC
Tru9I 1378 TTAA T^TAA
Tsp509I 1058 AATT ^AATT
TthHB8I 1293 TCGA T^CGA
XbaI 1356 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1189 GTCTAC GT^[AC][GT]AC
1258 GTATAC
AocI 12 CCTGAGG CC^T[ACGT]AGG
216 CCTGAGG
BalI 536 TGGCCA TGG^CCA
629 TGGCCA
BamHI 1202 GGATCC G^GATCC
1547 GGATCC
BsaAI 727 CACGTG [CT]AC^GT[GA]
1192 TACGTG
BsgI 1069 CTGCAC GTGCAG(16/14)
498 GTGCAG
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
216 CCTGAGG
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
216 CCTGAGG
EcoT14I 539 CCAAGG C^C[AT][AT]GG
1520 CCTTGG
FauI 300 CCCGC CCCGC(4/6)
317 CCCGC
HgaI 477 GACGC GACGC(5/10)
1306 GACGC
MluNI 536 TGGCCA TGG^CCA
629 TGGCCA
MscI 536 TGGCCA TGG^CCA
629 TGGCCA
PleI 240 GACTC GAGTC(4/5)
1275 GACTC
StyI 539 CCAAGG C^C[AT][AT]GG
1520 CCTTGG
XcmI 677 CCACATACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1337 CCAAGCGTGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AspI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TseI Tsp45I
TspRI Tth111I Van91I XhoII