Restriction map for
pF1KSDA2003
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 21 GACGCC G[GA]^CG[CT]C
Alw44I 1242 GTGCAC G^TGCAC
ApaLI 1242 GTGCAC G^TGCAC
Asp700I 285 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1242 GTGCAC G[AT]GC[AT]^C
BalI 296 TGGCCA TGG^CCA
BamHI 889 GGATCC G^GATCC
BbsI 231 GAAGAC GAAGAC(2/6)
BpuAI 231 GAAGAC GAAGAC(2/6)
BsaBI 160 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 21 GACGCC G[GA]^CG[CT]C
BsaI 85 GGTCTC GGTCTC(1/5)
BsaWI 620 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1242 GTGCAC G[AT]GC[AT]^C
BsmI 421 GCATTC GAATGC(1/-1)
BspHI 648 TCATGA T^CATGA
BsrDI 206 GCAATG GCAATG(2/0)
BstYI 889 GGATCC [GA]^GATC[CT]
Cfr13I 368 GGGCC G^G[ACGT]CC
EcoNI 90 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1298 GAATTC G^AATTC
EcoT14I 183 CCTTGG C^C[AT][AT]GG
HgaI 21 GACGC GACGC(5/10)
Hin1I 21 GACGCC G[GA]^CG[CT]C
MamI 160 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 889 GGATCC [GA]^GATC[CT]
MluNI 296 TGGCCA TGG^CCA
MscI 296 TGGCCA TGG^CCA
MwoI 206 GCAATGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
RcaI 648 TCATGA T^CATGA
Sau96I 368 GGGCC G^G[ACGT]CC
StyI 183 CCTTGG C^C[AT][AT]GG
XbaI 156 TCTAGA T^CTAGA
XhoII 889 GGATCC [GA]^GATC[CT]
XmnI 285 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 7 GCGG CCGC(-3/-1)
428 GCGG
AcsI 1188 AAATTC [GA]^AATT[CT]
1298 GAATTC
ApoI 1188 AAATTC [GA]^AATT[CT]
1298 GAATTC
BmyI 33 GGGCAC G[AGT]GC[ACT]^C
1242 GTGCAC
BsaJI 71 CCTGGG C^C[ACGT][ACGT]GG
183 CCTTGG
Bsp1286I 33 GGGCAC G[AGT]GC[ACT]^C
1242 GTGCAC
BspMI 235 ACCTGC ACCTGC(4/8)
268 ACCTGC
DdeI 16 CTGAG C^T[ACGT]AG
29 CTCAG
DpnII 890 GATC ^GATC
1142 GATC
HapII 621 CCGG C^CGG
1193 CCGG
HpaII 621 CCGG C^CGG
1193 CCGG
MboI 890 GATC ^GATC
1142 GATC
MboII 49 GAAGA GAAGA(8/7)
231 GAAGA
MslI 649 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
731 CATATGAGTG
MspA1I 5 CAGCGG C[AC]G^C[GT]G
426 CAGCGG
MspI 621 CCGG C^CGG
1193 CCGG
NdeI 483 CATATG CA^TATG
731 CATATG
NdeII 890 GATC ^GATC
1142 GATC
NlaIII 247 CATG CATG^
649 CATG
PstI 270 CTGCAG CTGCA^G
635 CTGCAG
Sau3AI 890 GATC ^GATC
1142 GATC
SfaNI 107 GATGC GCATC(5/9)
126 GATGC
TaqI 573 TCGA T^CGA
1308 TCGA
Tsp45I 40 GTGAC ^GT[GC]AC
377 GTCAC
TthHB8I 573 TCGA T^CGA
1308 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BpmI BsgI BsiYI BslI BsmAI
BsrI BssKI BstNI EaeI EcoRII
Fnu4HI HaeIII ItaI MaeI MaeIII
MnlI MseI MvaI NlaIV RsaI
ScrFI SfcI StuI Tru9I TseI
Tsp509I TspRI