Restriction map for
pF1KSDA2005
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AspEI AvaI
AviII BanII BbeI BbrPI BbvCI
BcgI BglI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstUI
Bsu36I CelII Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I FseI FspI HaeII Hin1I
HpaI KasI KspI MluI MroI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI StuI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 2239 GGTACC G^GTACC
AclI 206 AACGTT AA^CGTT
AflII 79 CTTAAG C^TTAAG
Asp718I 2239 GGTACC G^GTACC
AspHI 563 GAGCAC G[AT]GC[AT]^C
AvrII 4704 CCTAGG C^CTAGG
BamHI 3930 GGATCC G^GATCC
BciVI 2077 GTATCC GTATCC(6/5)
BfrI 79 CTTAAG C^TTAAG
BlnI 4704 CCTAGG C^CTAGG
BseRI 3973 GAGGAG GAGGAG(10/8)
BsiHKAI 563 GAGCAC G[AT]GC[AT]^C
BsmBI 3558 GAGACG CGTCTC(1/5)
BsmI 3244 GCATTC GAATGC(1/-1)
Bsp1407I 1990 TGTACA T^GTACA
BspMI 4400 GCAGGT ACCTGC(4/8)
BsrGI 1990 TGTACA T^GTACA
Bst1107I 3843 GTATAC GTA^TAC
BstZ17I 3843 GTATAC GTA^TAC
DraIII 2819 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 901 GACAGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 553 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 687 GAATTC G^AATTC
FauI 1906 CCCGC CCCGC(4/6)
HgaI 4566 GCGTC GACGC(5/10)
HincII 2740 GTTGAC GT[CT]^[GA]AC
HindII 2740 GTTGAC GT[CT]^[GA]AC
KpnI 2239 GGTACC GGTAC^C
MfeI 478 CAATTG C^AATTG
MspA1I 1479 CAGCTG C[AC]G^C[GT]G
MunI 478 CAATTG C^AATTG
NheI 1039 GCTAGC G^CTAGC
PflMI 2227 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 206 AACGTT AA^CGTT
PvuII 1479 CAGCTG CAG^CTG
SbfI 138 CCTGCAGG CCTGCA^GG
ScaI 4484 AGTACT AGT^ACT
SpeI 2851 ACTAGT A^CTAGT
Sse8387I 138 CCTGCAGG CCTGCA^GG
SspBI 1990 TGTACA T^GTACA
Van91I 2227 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3671 CCAAAAAACATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
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AciI 1907 CCGC CCGC(-3/-1)
3466 GCGG
AflIII 1436 ACATGT A^C[GA][CT]GT
3569 ACATGT
AseI 85 ATTAAT AT^TAAT
3171 ATTAAT
AsnI 85 ATTAAT AT^TAAT
3171 ATTAAT
AspI 1881 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4253 GACTAAGTC
BcnI 259 CCGGG CC^[GC]GG
2525 CCCGG
BglII 1773 AGATCT A^GATCT
3786 AGATCT
BmyI 563 GAGCAC G[AGT]GC[ACT]^C
3314 GGGCAC
BpmI 3967 CTCCAG CTGGAG(16/14)
2029 CTGGAG
BsaBI 358 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
668 GATTTGCATC
Bsp1286I 563 GAGCAC G[AGT]GC[ACT]^C
3314 GGGCAC
BspLU11I 1436 ACATGT A^CATGT
3569 ACATGT
BsrDI 653 GCAATG GCAATG(2/0)
1162 GCAATG
BstAPI 102 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2323 GCAGAAATTGC
BstEII 2352 GGTCACC G^GT[ACGT]ACC
3539 GGTCACC
BstPI 2352 GGTCACC G^GT[ACGT]ACC
3539 GGTCACC
BstXI 623 CCACGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1540 CCACATTTATGG
CfoI 3129 GCGC GCG^C
4392 GCGC
EcoO65I 2352 GGTCACC G^GT[ACGT]ACC
3539 GGTCACC
EcoRV 382 GATATC GAT^ATC
1051 GATATC
EcoT22I 2254 ATGCAT ATGCA^T
3242 ATGCAT
HapII 259 CCGG C^CGG
2526 CCGG
HhaI 3129 GCGC GCG^C
4392 GCGC
HinP1I 3129 GCGC G^CGC
4392 GCGC
HpaII 259 CCGG C^CGG
2526 CCGG
MaeII 207 ACGT A^CGT
3318 ACGT
MamI 358 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
668 GATTTGCATC
MspI 259 CCGG C^CGG
2526 CCGG
NciI 259 CCGGG CC^[GC]GG
2525 CCCGG
NdeI 3679 CATATG CA^TATG
4733 CATATG
NsiI 2254 ATGCAT ATGCA^T
3242 ATGCAT
PflFI 1881 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4253 GACTAAGTC
PpuMI 158 AGGACCT [GA]G^G[AT]CC[CT]
4272 AGGTCCT
PshBI 85 ATTAAT AT^TAAT
3171 ATTAAT
TaqI 2006 TCGA T^CGA
2064 TCGA
Tth111I 1881 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4253 GACTAAGTC
TthHB8I 2006 TCGA T^CGA
2064 TCGA
XbaI 1126 TCTAGA T^CTAGA
1533 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BalI BanI
BbsI BbvI BclI BfaI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BspHI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI