Restriction map for
pF1KSDB0003
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# NO CUT ENZYMES ( 130 enzymes) #
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Acc65I AccII AccIII AclI AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI BstBI BstUI BstXI BstYI
Bsu36I CelII CfoI ClaI CpoI
DraI DraII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoRV FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MflI MluI MluNI MroI
MscI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 694 GACGTC GACGT^C
AccI 1271 GTATAC GT^[AC][GT]AC
AcyI 694 GACGTC G[GA]^CG[CT]C
AfaI 323 GTAC GT^AC
AflII 1062 CTTAAG C^TTAAG
AlwNI 973 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1322 GGGCCC GGGCC^C
AvaI 81 CCCGGG C^[CT]CG[GA]G
AvaII 801 GGACC G^G[AT]CC
BbsI 451 GAAGAC GAAGAC(2/6)
BclI 137 TGATCA T^GATCA
BfrI 1062 CTTAAG C^TTAAG
BpuAI 451 GAAGAC GAAGAC(2/6)
BsaHI 694 GACGTC G[GA]^CG[CT]C
BsgI 436 CTGCAC GTGCAG(16/14)
BsiEI 91 CGGCCG CG[GA][CT]^CG
BsmI 200 GCATTC GAATGC(1/-1)
BsoBI 81 CCCGGG C^[CT]CG[GA]G
BspHI 1286 TCATGA T^CATGA
BspMI 440 ACCTGC ACCTGC(4/8)
BsrBI 1306 CCGCTC CCGCTC(-3/-3)
BsrFI 89 GCCGGC [GA]^CCGG[CT]
Bst1107I 1271 GTATAC GTA^TAC
BstAPI 1089 GCACTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 612 GGTGACC G^GT[ACGT]ACC
BstPI 612 GGTGACC G^GT[ACGT]ACC
BstZ17I 1271 GTATAC GTA^TAC
Cfr10I 89 GCCGGC [GA]^CCGG[CT]
DraIII 793 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 248 GACTAAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 91 CGGCCG [CT]^GGCC[GA]
EagI 91 CGGCCG C^GGCCG
EclXI 91 CGGCCG C^GGCCG
Eco52I 91 CGGCCG C^GGCCG
EcoO65I 612 GGTGACC G^GT[ACGT]ACC
EcoRI 1186 GAATTC G^AATTC
EcoT22I 395 ATGCAT ATGCA^T
FauI 1442 GCGGG CCCGC(4/6)
FbaI 137 TGATCA T^GATCA
Hin1I 694 GACGTC G[GA]^CG[CT]C
MaeII 695 ACGT A^CGT
MfeI 905 CAATTG C^AATTG
MslI 554 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 905 CAATTG C^AATTG
NaeI 89 GCCGGC GCC^GGC
NcoI 579 CCATGG C^CATGG
NdeI 475 CATATG CA^TATG
NgoMIV 89 GCCGGC G^CCGGC
NsiI 395 ATGCAT ATGCA^T
PleI 584 GACTC GAGTC(4/5)
PvuII 976 CAGCTG CAG^CTG
RcaI 1286 TCATGA T^CATGA
RsaI 323 GTAC GT^AC
SmaI 81 CCCGGG CCC^GGG
SmlI 1062 CTTAAG C^T[CT][GA]AG
SphI 911 GCATGC GCATG^C
TaqI 918 TCGA T^CGA
TthHB8I 918 TCGA T^CGA
XmaCI 81 CCCGGG C^CCGGG
XmaI 81 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BanI 98 GGTGCC G^G[CT][GA]CC
346 GGCACC
BanII 981 GGGCTC G[GA]GC[CT]^C
1322 GGGCCC
BmyI 981 GGGCTC G[AGT]GC[ACT]^C
1322 GGGCCC
BpmI 880 CTGGAG CTGGAG(16/14)
1069 CTGGAG
BseRI 75 CTCCTC GAGGAG(10/8)
1483 GAGGAG
BsmFI 122 GGGAC GGGAC(10/14)
1471 GGGAC
Bsp1286I 981 GGGCTC G[AGT]GC[ACT]^C
1322 GGGCCC
DsaI 282 CCGTGG C^C[GA][CT]GG
579 CCATGG
HindIII 375 AAGCTT A^AGCTT
733 AAGCTT
NspI 911 GCATGC [GA]CATG^[CT]
1358 ACATGC
PstI 178 CTGCAG CTGCA^G
782 CTGCAG
SapI 1395 GAAGAGC GCTCTTC(1/4)
1308 GCTCTTC
Tsp45I 613 GTGAC ^GT[GC]AC
697 GTCAC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BcnI BfaI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI