Restriction map for
pF1KSDB0005
##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI AseI AsnI Asp718I AspI
AvrII BbrPI BclI BfrI BlnI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstZ17I ClaI DraI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV FbaI FseI HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacII
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3316 GACGTC GACGT^C
AcsI 2881 GAATTT [GA]^AATT[CT]
AflIII 2531 ACACGT A^C[GA][CT]GT
AhdI 3697 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2506 GTGCAC G^TGCAC
AocI 2913 CCTGAGG CC^T[ACGT]AGG
ApaLI 2506 GTGCAC G^TGCAC
ApoI 2881 GAATTT [GA]^AATT[CT]
AspEI 3697 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 890 TGCGCA TGC^GCA
BbvCI 1395 CCTCAGC CCTCAGC(-5/-2)
BfaI 1523 CTAG C^TAG
BlpI 2860 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2860 GCTGAGC GC^T[ACGT]AGC
BsaAI 529 TACGTG [CT]AC^GT[GA]
BsaBI 2364 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3274 TCCGGT [AT]^CCGG[AT]
BsiEI 429 CGACCG CG[GA][CT]^CG
BsmBI 1271 GAGACG CGTCTC(1/5)
BsmI 2681 GCATTC GAATGC(1/-1)
BssHII 234 GCGCGC G^CGCGC
BstEII 3174 GGTCACC G^GT[ACGT]ACC
BstPI 3174 GGTCACC G^GT[ACGT]ACC
Bsu36I 2913 CCTGAGG CC^T[ACGT]AGG
CelII 2860 GCTGAGC GC^T[ACGT]AGC
CpoI 590 CGGACCG CG^G[AT]CCG
Eam1105I 3697 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2913 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3174 GGTCACC G^GT[ACGT]ACC
EcoT22I 674 ATGCAT ATGCA^T
FspI 890 TGCGCA TGC^GCA
HindIII 2610 AAGCTT A^AGCTT
MaeI 1523 CTAG C^TAG
MamI 2364 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2768 TTAA T^TAA
NaeI 1340 GCCGGC GCC^GGC
NgoMIV 1340 GCCGGC G^CCGGC
NheI 1522 GCTAGC G^CTAGC
NsiI 674 ATGCAT ATGCA^T
PaeR7I 660 CTCGAG C^TCGAG
RsrII 590 CGGACCG CG^G[AT]CCG
SacI 1654 GAGCTC GAGCT^C
SalI 724 GTCGAC G^TCGAC
SbfI 3027 CCTGCAGG CCTGCA^GG
SfiI 803 GGCCCCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3488 GCATGC GCATG^C
Sse8387I 3027 CCTGCAGG CCTGCA^GG
StuI 116 AGGCCT AGG^CCT
Tru9I 2768 TTAA T^TAA
Tsp509I 2882 AATT ^AATT
XhoI 660 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 724 GTCGAC GT^[AC][GT]AC
2845 GTCTAC
Aor51HI 107 AGCGCT AGC^GCT
155 AGCGCT
ApaI 879 GGGCCC GGGCC^C
2162 GGGCCC
Asp700I 519 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2598 GAAGTGTTTC
BamHI 479 GGATCC G^GATCC
1893 GGATCC
BbsI 2182 GTCTTC GAAGAC(2/6)
3151 GTCTTC
BciVI 1303 GGATAC GTATCC(6/5)
2918 GGATAC
BglII 1235 AGATCT A^GATCT
2369 AGATCT
BpuAI 2182 GTCTTC GAAGAC(2/6)
3151 GTCTTC
BsaI 1630 GAGACC GGTCTC(1/5)
1431 GGTCTC
BssSI 550 CACGAG CACGAG(-5/-1)
2652 CTCGTG
BstAPI 177 GCAGACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3021 GCAGGCCCTGC
Eco47III 107 AGCGCT AGC^GCT
155 AGCGCT
HincII 571 GTCAAC GT[CT]^[GA]AC
724 GTCGAC
HindII 571 GTCAAC GT[CT]^[GA]AC
724 GTCGAC
NspI 1082 GCATGT [GA]CATG^[CT]
3488 GCATGC
SapI 201 GAAGAGC GCTCTTC(1/4)
1689 GCTCTTC
SmlI 660 CTCGAG C^T[CT][GA]AG
2218 CTCAAG
TfiI 1514 GAATC G^A[AT]TC
2806 GATTC
XmnI 519 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2598 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcgI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI