Restriction map for
pF1KSDB0012
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BcgI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfcI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 1024 GAATTT [GA]^AATT[CT]
ApaI 59 GGGCCC GGGCC^C
ApoI 1024 GAATTT [GA]^AATT[CT]
AvaI 516 CCCGAG C^[CT]CG[GA]G
BalI 107 TGGCCA TGG^CCA
BbeI 438 GGCGCC GGCGC^C
BbsI 1371 GAAGAC GAAGAC(2/6)
BciVI 666 GGATAC GTATCC(6/5)
BpuAI 1371 GAAGAC GAAGAC(2/6)
BsaI 512 GAGACC GGTCTC(1/5)
BsiEI 507 CGACCG CG[GA][CT]^CG
BsmBI 620 GAGACG CGTCTC(1/5)
BsoBI 516 CCCGAG C^[CT]CG[GA]G
Bsp1407I 960 TGTACA T^GTACA
BsrBI 1307 GAGCGG CCGCTC(-3/-3)
BsrDI 676 GCAATG GCAATG(2/0)
BsrGI 960 TGTACA T^GTACA
DpnII 1063 GATC ^GATC
KasI 438 GGCGCC G^GCGCC
MboI 1063 GATC ^GATC
MluNI 107 TGGCCA TGG^CCA
MscI 107 TGGCCA TGG^CCA
MslI 970 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 438 GGCGCC GG^CGCC
NcoI 1139 CCATGG C^CATGG
NdeI 970 CATATG CA^TATG
NdeII 1063 GATC ^GATC
PflMI 730 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 996 GCTCTTC GCTCTTC(1/4)
Sau3AI 1063 GATC ^GATC
ScaI 1263 AGTACT AGT^ACT
SfoI 438 GGCGCC GGC^GCC
SmlI 256 CTCAAG C^T[CT][GA]AG
SspBI 960 TGTACA T^GTACA
Van91I 730 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 464 CCAGGAATGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 266 GGCGTC G[GA]^CG[CT]C
438 GGCGCC
AlwNI 446 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
953 CAGAAGCTG
Asp700I 936 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1144 GAATTGATTC
BbvCI 718 GCTGAGG CCTCAGC(-5/-2)
1093 GCTGAGG
BpmI 538 CTCCAG CTGGAG(16/14)
280 CTGGAG
BsaHI 266 GGCGTC G[GA]^CG[CT]C
438 GGCGCC
BsgI 204 CTGCAC GTGCAG(16/14)
295 GTGCAG
BspMI 13 ACCTGC ACCTGC(4/8)
92 ACCTGC
EcoT14I 735 CCTTGG C^C[AT][AT]GG
1139 CCATGG
HaeII 438 GGCGCC [GA]GCGC^[CT]
447 AGCGCC
HgaI 43 GACGC GACGC(5/10)
267 GCGTC
Hin1I 266 GGCGTC G[GA]^CG[CT]C
438 GGCGCC
MaeII 7 ACGT A^CGT
272 ACGT
MaeIII 124 GTGAC ^GT[ACGT]AC
269 GTCAC
MseI 863 TTAA T^TAA
875 TTAA
MspA1I 55 CAGCGG C[AC]G^C[GT]G
160 CAGCGG
NspI 50 ACATGC [GA]CATG^[CT]
1331 ACATGC
SfiI 108 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
402 GGCCATCAAGGCC
StyI 735 CCTTGG C^C[AT][AT]GG
1139 CCATGG
Tru9I 863 TTAA T^TAA
875 TTAA
Tsp45I 124 GTGAC ^GT[GC]AC
269 GTCAC
XmnI 936 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1144 GAATTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbvI BcnI BglI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PpuMI
RsaI Sau96I ScrFI SfaNI TfiI
TseI Tsp509I TspRI