Restriction map for
pF1KSDB0017
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# NO CUT ENZYMES ( 57 enzymes) #
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Acc65I AclI AflII AgeI AscI
Asp718I AvrII BamHI BclI BfrI
BglII BlnI BsaBI BsiWI BsmI
BspDI BspHI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI EcoO65I EcoT22I FbaI
HpaI KpnI MamI MfeI MunI
NdeI NheI NsiI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI SalI SfiI SfuI SgrAI
SmlI SnaBI SphI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 2844 GACGTC GACGT^C
Alw44I 5101 GTGCAC G^TGCAC
AocI 4033 CCTGAGG CC^T[ACGT]AGG
ApaLI 5101 GTGCAC G^TGCAC
AseI 2316 ATTAAT AT^TAAT
AsnI 2316 ATTAAT AT^TAAT
AviII 1382 TGCGCA TGC^GCA
BbrPI 4807 CACGTG CAC^GTG
BbvCI 3374 CCTCAGC CCTCAGC(-5/-2)
BcgI 3866 GCATCCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2837 GGATAC GTATCC(6/5)
BsaAI 4807 CACGTG [CT]AC^GT[GA]
BsmBI 3885 CGTCTC CGTCTC(1/5)
BspLU11I 824 ACATGT A^CATGT
BstXI 4679 CCAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4033 CCTGAGG CC^T[ACGT]AGG
CpoI 622 CGGACCG CG^G[AT]CCG
EagI 277 CGGCCG C^GGCCG
EclXI 277 CGGCCG C^GGCCG
Eco52I 277 CGGCCG C^GGCCG
Eco81I 4033 CCTGAGG CC^T[ACGT]AGG
EcoRI 523 GAATTC G^AATTC
EcoRV 4999 GATATC GAT^ATC
FseI 3901 GGCCGGCC GGCCGG^CC
FspI 1382 TGCGCA TGC^GCA
HincII 1161 GTTGAC GT[CT]^[GA]AC
HindII 1161 GTTGAC GT[CT]^[GA]AC
MluI 1750 ACGCGT A^CGCGT
NotI 276 GCGGCCGC GC^GGCCGC
NruI 1817 TCGCGA TCG^CGA
PmaCI 4807 CACGTG CAC^GTG
PmlI 4807 CACGTG CAC^GTG
PshBI 2316 ATTAAT AT^TAAT
RsrII 622 CGGACCG CG^G[AT]CCG
ScaI 2809 AGTACT AGT^ACT
SpeI 2812 ACTAGT A^CTAGT
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 2365 GTCTAC GT^[AC][GT]AC
4744 GTCTAC
AhdI 1399 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4729 GACTTTGTGTC
Aor51HI 3584 AGCGCT AGC^GCT
3765 AGCGCT
ApaI 2801 GGGCCC GGGCC^C
3236 GGGCCC
AspEI 1399 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4729 GACTTTGTGTC
Bsp1407I 1724 TGTACA T^GTACA
3614 TGTACA
BsrGI 1724 TGTACA T^GTACA
3614 TGTACA
BstAPI 294 GCACGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3972 GCAGACTCTGC
DraIII 226 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
295 CACGCGGTG
Eam1105I 1399 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4729 GACTTTGTGTC
Eco47III 3584 AGCGCT AGC^GCT
3765 AGCGCT
HindIII 3061 AAGCTT A^AGCTT
4771 AAGCTT
KspI 122 CCGCGG CCGC^GG
2582 CCGCGG
NcoI 977 CCATGG C^CATGG
3270 CCATGG
PshAI 1143 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1683 GACAGCCGTC
SacII 122 CCGCGG CCGC^GG
2582 CCGCGG
SexAI 1505 ACCAGGT A^CC[AT]GGT
4841 ACCTGGT
SspBI 1724 TGTACA T^GTACA
3614 TGTACA
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# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
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AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfoI SmaI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI