Restriction map for
pF1KSDB0022
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbsI BcgI BciVI
BclI BfrI BlnI BpuAI BsaHI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 906 GTCTAC GT^[AC][GT]AC
AgeI 1323 ACCGGT A^CCGGT
AlwNI 1502 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1471 GGGCCC GGGCC^C
Asp700I 270 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 710 TGGCCA TGG^CCA
BamHI 1426 GGATCC G^GATCC
BanI 435 GGTGCC G^G[CT][GA]CC
BbrPI 1267 CACGTG CAC^GTG
BglI 585 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 870 AGATCT A^GATCT
BsaAI 1267 CACGTG [CT]AC^GT[GA]
BsaBI 15 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1323 ACCGGT [AT]^CCGG[AT]
BsgI 1022 GTGCAG GTGCAG(16/14)
BsmBI 1435 GAGACG CGTCTC(1/5)
BspMI 806 ACCTGC ACCTGC(4/8)
BsrBI 40 GAGCGG CCGCTC(-3/-3)
BsrDI 1076 GCAATG GCAATG(2/0)
BsrFI 1323 ACCGGT [GA]^CCGG[CT]
BssSI 226 CACGAG CACGAG(-5/-1)
Cfr10I 1323 ACCGGT [GA]^CCGG[CT]
DraII 965 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 805 GACCTGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 526 CCGCGG C^C[GA][CT]GG
EcoO109I 965 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 891 CCTTGG C^C[AT][AT]GG
EcoT22I 844 ATGCAT ATGCA^T
HgaI 353 GACGC GACGC(5/10)
KspI 526 CCGCGG CCGC^GG
MamI 15 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 710 TGGCCA TGG^CCA
MscI 710 TGGCCA TGG^CCA
MseI 841 TTAA T^TAA
NsiI 844 ATGCAT ATGCA^T
PinAI 1323 ACCGGT A^CCGGT
PmaCI 1267 CACGTG CAC^GTG
PmlI 1267 CACGTG CAC^GTG
PstI 460 CTGCAG CTGCA^G
SacII 526 CCGCGG CCGC^GG
SapI 378 GCTCTTC GCTCTTC(1/4)
SexAI 1217 ACCTGGT A^CC[AT]GGT
SmaI 1248 CCCGGG CCC^GGG
SmlI 199 CTCAAG C^T[CT][GA]AG
StyI 891 CCTTGG C^C[AT][AT]GG
Tru9I 841 TTAA T^TAA
XmaCI 1248 CCCGGG C^CCGGG
XmaI 1248 CCCGGG C^CCGGG
XmnI 270 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 355 CGCG CG^CG
527 CGCG
AocI 261 CCTGAGG CC^T[ACGT]AGG
1507 CCTGAGG
BfaI 599 CTAG C^TAG
1072 CTAG
BlpI 102 GCTGAGC GC^T[ACGT]AGC
1520 GCTCAGC
BpmI 1364 CTCCAG CTGGAG(16/14)
337 CTGGAG
Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC
1520 GCTCAGC
BstEII 489 GGTCACC G^GT[ACGT]ACC
622 GGTGACC
BstPI 489 GGTCACC G^GT[ACGT]ACC
622 GGTGACC
BstUI 355 CGCG CG^CG
527 CGCG
BstXI 885 CCAGCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
923 CCACCCCAGTGG
Bsu36I 261 CCTGAGG CC^T[ACGT]AGG
1507 CCTGAGG
CelII 102 GCTGAGC GC^T[ACGT]AGC
1520 GCTCAGC
EaeI 592 CGGCCA [CT]^GGCC[GA]
710 TGGCCA
Eco81I 261 CCTGAGG CC^T[ACGT]AGG
1507 CCTGAGG
EcoNI 333 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
726 CCTAAACAAGG
EcoO65I 489 GGTCACC G^GT[ACGT]ACC
622 GGTGACC
HaeII 386 GGCGCT [GA]GCGC^[CT]
887 AGCGCC
HincII 396 GTCAAC GT[CT]^[GA]AC
1014 GTTGAC
HindII 396 GTCAAC GT[CT]^[GA]AC
1014 GTTGAC
MaeI 599 CTAG C^TAG
1072 CTAG
MaeII 674 ACGT A^CGT
1268 ACGT
MvnI 355 CGCG CG^CG
527 CGCG
PleI 313 GACTC GAGTC(4/5)
812 GAGTC
SfcI 460 CTGCAG C^T[GA][CT]AG
556 CTACAG
TaqI 254 TCGA T^CGA
371 TCGA
TfiI 15 GATTC G^A[AT]TC
29 GAATC
TthHB8I 254 TCGA T^CGA
371 TCGA
XcmI 173 CCAGCACCGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
476 CCAGCGGCTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvCI BbvI
BcnI BmyI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
XhoII