Restriction map for
pF1KSDB0027
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AflII AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BciVI BclI BfaI BfrI
BlnI BseAI BsiWI BsmBI BspDI
BspEI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI PvuI RsrII SalI
SapI SbfI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1417 GACGTC GACGT^C
AccI 2089 GTCTAC GT^[AC][GT]AC
AclI 1081 AACGTT AA^CGTT
AcsI 1386 GAATTT [GA]^AATT[CT]
AhdI 331 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2658 GTGCAC G^TGCAC
ApaLI 2658 GTGCAC G^TGCAC
ApoI 1386 GAATTT [GA]^AATT[CT]
Asp700I 864 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 331 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2045 TGGCCA TGG^CCA
BamHI 5 GGATCC G^GATCC
BbeI 1531 GGCGCC GGCGC^C
BbrPI 2239 CACGTG CAC^GTG
BcgI 2720 GCATGATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 731 AGATCT A^GATCT
BsaBI 1170 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 839 TCCGGT [AT]^CCGG[AT]
BsmI 602 GCATTC GAATGC(1/-1)
Bsp1407I 2141 TGTACA T^GTACA
BspLU11I 2819 ACATGT A^CATGT
BsrGI 2141 TGTACA T^GTACA
BssSI 1315 CACGAG CACGAG(-5/-1)
DraIII 1666 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1837 GACACCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 331 GACTCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 2734 GTCAAC GT[CT]^[GA]AC
HindII 2734 GTCAAC GT[CT]^[GA]AC
KasI 1531 GGCGCC G^GCGCC
MamI 1170 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2045 TGGCCA TGG^CCA
MscI 2045 TGGCCA TGG^CCA
NarI 1531 GGCGCC GG^CGCC
NcoI 275 CCATGG C^CATGG
PmaCI 2239 CACGTG CAC^GTG
PmlI 2239 CACGTG CAC^GTG
PshAI 437 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1081 AACGTT AA^CGTT
ScaI 1196 AGTACT AGT^ACT
SexAI 2525 ACCTGGT A^CC[AT]GGT
SfiI 180 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1531 GGCGCC GGC^GCC
SmaI 2739 CCCGGG CCC^GGG
SspBI 2141 TGTACA T^GTACA
SspI 904 AATATT AAT^ATT
TfiI 2832 GATTC G^A[AT]TC
XmaCI 2739 CCCGGG C^CCGGG
XmaI 2739 CCCGGG C^CCGGG
XmnI 864 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 2606 ACGTGT A^C[GA][CT]GT
2819 ACATGT
Aor51HI 235 AGCGCT AGC^GCT
2096 AGCGCT
ApaI 14 GGGCCC GGGCC^C
884 GGGCCC
BbvCI 1821 CCTCAGC CCTCAGC(-5/-2)
1540 GCTGAGG
BpmI 1786 CTGGAG CTGGAG(16/14)
2026 CTGGAG
BsaAI 2239 CACGTG [CT]AC^GT[GA]
2605 TACGTG
BsiEI 84 CGGCCG CG[GA][CT]^CG
2592 CGGCCG
BsrBI 1629 CCGCTC CCGCTC(-3/-3)
1324 GAGCGG
BsrDI 1536 CATTGC GCAATG(2/0)
2276 CATTGC
BstAPI 297 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
309 GCAGGTCCTGC
EagI 84 CGGCCG C^GGCCG
2592 CGGCCG
EclXI 84 CGGCCG C^GGCCG
2592 CGGCCG
Eco47III 235 AGCGCT AGC^GCT
2096 AGCGCT
Eco52I 84 CGGCCG C^GGCCG
2592 CGGCCG
KspI 185 CCGCGG CCGC^GG
2709 CCGCGG
MseI 680 TTAA T^TAA
2540 TTAA
SacI 1990 GAGCTC GAGCT^C
2322 GAGCTC
SacII 185 CCGCGG CCGC^GG
2709 CCGCGG
TaqI 920 TCGA T^CGA
2302 TCGA
Tru9I 680 TTAA T^TAA
2540 TTAA
TthHB8I 920 TCGA T^CGA
2302 TCGA
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BglI BlpI BmyI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII