Restriction map for
pF1KSDB0030
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# NO CUT ENZYMES ( 61 enzymes) #
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AccIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BclI BlnI BseAI BsiWI
Bsp1407I BspEI BsrGI BssHII BstBI
BstEII BstPI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FbaI FseI HindIII KspI MluI
MroI NotI NruI NspV PacI
PaeR7I PflFI PmeI PshBI PvuI
RsrII SacI SacII SalI SexAI
SfiI SfuI SnaBI SphI SspBI
SspI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 2931 GACGTC GACGT^C
Acc65I 3408 GGTACC G^GTACC
AclI 1850 AACGTT AA^CGTT
Asp700I 3381 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3408 GGTACC G^GTACC
AspHI 3067 GAGCAC G[AT]GC[AT]^C
AviII 3632 TGCGCA TGC^GCA
BbrPI 4024 CACGTG CAC^GTG
BbvCI 3997 GCTGAGG CCTCAGC(-5/-2)
BcgI 4544 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4441 GTATCC GTATCC(6/5)
BsaAI 4024 CACGTG [CT]AC^GT[GA]
BsaBI 2251 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1857 CGACCG CG[GA][CT]^CG
BsiHKAI 3067 GAGCAC G[AT]GC[AT]^C
BsmI 4264 GCATTC GAATGC(1/-1)
BspLU11I 2471 ACATGT A^CATGT
BssSI 3899 CTCGTG CACGAG(-5/-1)
Bst1107I 4290 GTATAC GTA^TAC
BstZ17I 4290 GTATAC GTA^TAC
DraIII 1081 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2773 GACTGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 563 GAATTC G^AATTC
EcoRV 592 GATATC GAT^ATC
EcoT22I 2767 ATGCAT ATGCA^T
FspI 3632 TGCGCA TGC^GCA
HincII 2751 GTTAAC GT[CT]^[GA]AC
HindII 2751 GTTAAC GT[CT]^[GA]AC
HpaI 2751 GTTAAC GTT^AAC
KpnI 3408 GGTACC GGTAC^C
MamI 2251 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2985 CAATTG C^AATTG
MunI 2985 CAATTG C^AATTG
NaeI 2106 GCCGGC GCC^GGC
NcoI 4082 CCATGG C^CATGG
NdeI 3907 CATATG CA^TATG
NgoMIV 2106 GCCGGC G^CCGGC
NheI 3643 GCTAGC G^CTAGC
NsiI 2767 ATGCAT ATGCA^T
NspI 2471 ACATGT [GA]CATG^[CT]
PflMI 2071 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4024 CACGTG CAC^GTG
PmlI 4024 CACGTG CAC^GTG
Psp1406I 1850 AACGTT AA^CGTT
SbfI 1666 CCTGCAGG CCTGCA^GG
SgrAI 86 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3370 ACTAGT A^CTAGT
Sse8387I 1666 CCTGCAGG CCTGCA^GG
Van91I 2071 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3381 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccII 3551 CGCG CG^CG
4538 CGCG
AflII 1263 CTTAAG C^TTAAG
2791 CTTAAG
AgeI 722 ACCGGT A^CCGGT
1020 ACCGGT
BamHI 271 GGATCC G^GATCC
1977 GGATCC
BbeI 448 GGCGCC GGCGC^C
1317 GGCGCC
BbsI 1753 GAAGAC GAAGAC(2/6)
2928 GAAGAC
BfrI 1263 CTTAAG C^TTAAG
2791 CTTAAG
BglII 299 AGATCT A^GATCT
1125 AGATCT
BlpI 4221 GCTTAGC GC^T[ACGT]AGC
4729 GCTGAGC
Bpu1102I 4221 GCTTAGC GC^T[ACGT]AGC
4729 GCTGAGC
BpuAI 1753 GAAGAC GAAGAC(2/6)
2928 GAAGAC
BsaWI 722 ACCGGT [AT]^CCGG[AT]
1020 ACCGGT
BsmBI 685 GAGACG CGTCTC(1/5)
1563 GAGACG
BspDI 2555 ATCGAT AT^CGAT
3253 ATCGAT
BspHI 1095 TCATGA T^CATGA
4466 TCATGA
BspMI 3558 ACCTGC ACCTGC(4/8)
4640 ACCTGC
BsrBI 3505 GAGCGG CCGCTC(-3/-3)
3595 GAGCGG
BstUI 3551 CGCG CG^CG
4538 CGCG
CelII 4221 GCTTAGC GC^T[ACGT]AGC
4729 GCTGAGC
ClaI 2555 ATCGAT AT^CGAT
3253 ATCGAT
KasI 448 GGCGCC G^GCGCC
1317 GGCGCC
MvnI 3551 CGCG CG^CG
4538 CGCG
NarI 448 GGCGCC GG^CGCC
1317 GGCGCC
PinAI 722 ACCGGT A^CCGGT
1020 ACCGGT
PshAI 3106 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4470 GACGGCTGTC
RcaI 1095 TCATGA T^CATGA
4466 TCATGA
SapI 2665 GAAGAGC GCTCTTC(1/4)
3843 GCTCTTC
ScaI 3373 AGTACT AGT^ACT
3962 AGTACT
SfoI 448 GGCGCC GGC^GCC
1317 GGCGCC
SmaI 72 CCCGGG CCC^GGG
1786 CCCGGG
XcmI 2447 CCAACTTCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2873 CCAAGCCCCGAATGG
XmaCI 72 CCCGGG C^CCGGG
1786 CCCGGG
XmaI 72 CCCGGG C^CCGGG
1786 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII