Restriction map for
pF1KSDB0034
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AseI AsnI Asp718I
AspEI AviII AvrII BalI BbrPI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BstBI BstEII
BstPI CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 1206 GTATAC GT^[AC][GT]AC
AflIII 1475 ACACGT A^C[GA][CT]GT
AscI 1721 GGCGCGCC GG^CGCGCC
AspI 1813 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 674 GGATCC G^GATCC
BbeI 1621 GGCGCC GGCGC^C
BfaI 1781 CTAG C^TAG
BglII 680 AGATCT A^GATCT
BsaAI 1476 CACGTA [CT]AC^GT[GA]
BsiWI 1478 CGTACG C^GTACG
BspHI 1505 TCATGA T^CATGA
Bst1107I 1206 GTATAC GTA^TAC
BstZ17I 1206 GTATAC GTA^TAC
DraIII 700 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1570 GACAGCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI 1621 GGCGCC G^GCGCC
MaeI 1781 CTAG C^TAG
NarI 1621 GGCGCC GG^CGCC
NheI 1780 GCTAGC G^CTAGC
PflFI 1813 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 40 CAGCTG CAG^CTG
RcaI 1505 TCATGA T^CATGA
SbfI 1518 CCTGCAGG CCTGCA^GG
SexAI 587 ACCTGGT A^CC[AT]GGT
SfiI 768 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1621 GGCGCC GGC^GCC
Sse8387I 1518 CCTGCAGG CCTGCA^GG
TfiI 461 GAATC G^A[AT]TC
Tsp509I 804 AATT ^AATT
Tth111I 1813 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1454 CCAGGTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcyI 688 GACGCC G[GA]^CG[CT]C
1621 GGCGCC
AocI 466 CCTGAGG CC^T[ACGT]AGG
1921 CCTAAGG
Asp700I 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1335 GAAGAGGTTC
AvaI 84 CTCGGG C^[CT]CG[GA]G
1933 CCCGAG
BbsI 79 GTCTTC GAAGAC(2/6)
961 GTCTTC
BcgI 1443 CGAGGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2079 CGAGGACAGTGC
BglI 769 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1930 GCCCCCGAGGC
BpuAI 79 GTCTTC GAAGAC(2/6)
961 GTCTTC
BsaBI 675 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1365 GATCAGCATC
BsaHI 688 GACGCC G[GA]^CG[CT]C
1621 GGCGCC
BsaI 1216 GAGACC GGTCTC(1/5)
1737 GGTCTC
BsoBI 84 CTCGGG C^[CT]CG[GA]G
1933 CCCGAG
BsrFI 765 GCCGGC [GA]^CCGG[CT]
1876 GCCGGC
BssHII 724 GCGCGC G^CGCGC
1722 GCGCGC
BssSI 209 CACGAG CACGAG(-5/-1)
142 CTCGTG
BstXI 1727 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2019 CCAGGAGACTGG
Bsu36I 466 CCTGAGG CC^T[ACGT]AGG
1921 CCTAAGG
Cfr10I 765 GCCGGC [GA]^CCGG[CT]
1876 GCCGGC
EaeI 767 CGGCCA [CT]^GGCC[GA]
1613 TGGCCG
EarI 94 CTCTTC CTCTTC(1/4)
1335 GAAGAG
Eco81I 466 CCTGAGG CC^T[ACGT]AGG
1921 CCTAAGG
EcoNI 852 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1224 CCTGCACCAGG
HaeII 1353 GGCGCT [GA]GCGC^[CT]
1621 GGCGCC
Hin1I 688 GACGCC G[GA]^CG[CT]C
1621 GGCGCC
Ksp632I 94 CTCTTC CTCTTC(1/4)
1335 GAAGAG
KspI 634 CCGCGG CCGC^GG
1538 CCGCGG
MamI 675 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1365 GATCAGCATC
NaeI 765 GCCGGC GCC^GGC
1876 GCCGGC
NgoMIV 765 GCCGGC G^CCGGC
1876 GCCGGC
NspI 512 GCATGT [GA]CATG^[CT]
1178 ACATGC
PpuMI 200 AGGTCCC [GA]G^G[AT]CC[CT]
1283 GGGTCCT
SacI 268 GAGCTC GAGCT^C
625 GAGCTC
SacII 634 CCGCGG CCGC^GG
1538 CCGCGG
SmlI 325 CTCAAG C^T[CT][GA]AG
1384 CTCAAG
StuI 1833 AGGCCT AGG^CCT
1937 AGGCCT
XmnI 90 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1335 GAAGAGGTTC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XhoII