Restriction map for
pF1KSDB0035
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Asp700I 664 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 619 GAGCTC G[AT]GC[AT]^C
AvaI 713 CCCGGG C^[CT]CG[GA]G
BanII 619 GAGCTC G[GA]GC[CT]^C
BsaWI 1131 TCCGGT [AT]^CCGG[AT]
BsiHKAI 619 GAGCTC G[AT]GC[AT]^C
BsmFI 985 GTCCC GGGAC(10/14)
BsmI 24 GAATGC GAATGC(1/-1)
BsoBI 713 CCCGGG C^[CT]CG[GA]G
BsrDI 1193 GCAATG GCAATG(2/0)
DraIII 179 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 976 GACCTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1241 CGGCCA [CT]^GGCC[GA]
HaeII 510 AGCGCC [GA]GCGC^[CT]
HincII 379 GTCAAC GT[CT]^[GA]AC
HindII 379 GTCAAC GT[CT]^[GA]AC
HindIII 1353 AAGCTT A^AGCTT
MaeII 911 ACGT A^CGT
PstI 1220 CTGCAG CTGCA^G
SacI 619 GAGCTC GAGCT^C
SfcI 1220 CTGCAG C^T[GA][CT]AG
SmaI 713 CCCGGG CCC^GGG
SmlI 877 CTTGAG C^T[CT][GA]AG
StuI 1397 AGGCCT AGG^CCT
XbaI 834 TCTAGA T^CTAGA
XmaCI 713 CCCGGG C^CCGGG
XmaI 713 CCCGGG C^CCGGG
XmnI 664 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AocI 1027 CCTGAGG CC^T[ACGT]AGG
1341 CCTCAGG
BpmI 1074 CTCCAG CTGGAG(16/14)
806 CTGGAG
BseRI 701 CTCCTC GAGGAG(10/8)
722 CTCCTC
BsiYI 928 CCTTTAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
981 CCCTGTCCCGG
BslI 928 CCTTTAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
981 CCCTGTCCCGG
BstXI 241 CCACCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
421 CCAGACGAATGG
Bsu36I 1027 CCTGAGG CC^T[ACGT]AGG
1341 CCTCAGG
CfoI 225 GCGC GCG^C
511 GCGC
Eco81I 1027 CCTGAGG CC^T[ACGT]AGG
1341 CCTCAGG
EcoRI 273 GAATTC G^AATTC
416 GAATTC
HgaI 823 GACGC GACGC(5/10)
1014 GACGC
HhaI 225 GCGC GCG^C
511 GCGC
HinP1I 225 GCGC G^CGC
511 GCGC
MslI 242 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CATACCAATG
MspA1I 596 CAGCGG C[AC]G^C[GT]G
766 CCGCTG
NspI 460 GCATGT [GA]CATG^[CT]
1068 ACATGC
PshAI 193 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1225 GACACCAGTC
TaqI 33 TCGA T^CGA
623 TCGA
TthHB8I 33 TCGA T^CGA
623 TCGA
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp718I BanI
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI KpnI MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII