Restriction map for
pF1KSDB0046
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BcgI BclI BfrI BlnI BsaAI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BssHII BstBI BstUI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FbaI FseI
FspI HaeII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 5331 GTATAC GT^[AC][GT]AC
AcyI 4128 GACGCC G[GA]^CG[CT]C
AgeI 5134 ACCGGT A^CCGGT
Asp700I 5448 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3108 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3168 GGATCC G^GATCC
BanI 2795 GGCACC G^G[CT][GA]CC
BlpI 2637 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2637 GCTAAGC GC^T[ACGT]AGC
BsaHI 4128 GACGCC G[GA]^CG[CT]C
BsaI 2899 GAGACC GGTCTC(1/5)
BsaWI 5134 ACCGGT [AT]^CCGG[AT]
BsmBI 236 GAGACG CGTCTC(1/5)
BsmI 2146 GAATGC GAATGC(1/-1)
Bsp1407I 750 TGTACA T^GTACA
BspMI 780 ACCTGC ACCTGC(4/8)
BsrBI 5250 CCGCTC CCGCTC(-3/-3)
BsrFI 5134 ACCGGT [GA]^CCGG[CT]
BsrGI 750 TGTACA T^GTACA
Bst1107I 5331 GTATAC GTA^TAC
BstZ17I 5331 GTATAC GTA^TAC
CelII 2637 GCTAAGC GC^T[ACGT]AGC
CfoI 4902 GCGC GCG^C
Cfr10I 5134 ACCGGT [GA]^CCGG[CT]
DraIII 2024 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 1453 ATGCAT ATGCA^T
HhaI 4902 GCGC GCG^C
Hin1I 4128 GACGCC G[GA]^CG[CT]C
HinP1I 4902 GCGC G^CGC
MfeI 3842 CAATTG C^AATTG
MunI 3842 CAATTG C^AATTG
NdeI 575 CATATG CA^TATG
NsiI 1453 ATGCAT ATGCA^T
PflFI 3108 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5134 ACCGGT A^CCGGT
SacI 5665 GAGCTC GAGCT^C
SgrAI 5133 CACCGGTG C[GA]^CCGG[CT]G
SspBI 750 TGTACA T^GTACA
Tth111I 3108 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 108 TCTAGA T^CTAGA
XmnI 5448 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 4076 CCCGAG C^[CT]CG[GA]G
4337 CCCGAG
BbvCI 349 CCTCAGC CCTCAGC(-5/-2)
3697 CCTCAGC
BciVI 1653 GGATAC GTATCC(6/5)
1712 GTATCC
BcnI 3744 CCGGG CC^[GC]GG
5444 CCCGG
BglI 4197 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4268 GCCCCTTGGGC
BglII 1095 AGATCT A^GATCT
2457 AGATCT
BsoBI 4076 CCCGAG C^[CT]CG[GA]G
4337 CCCGAG
BspHI 1386 TCATGA T^CATGA
5684 TCATGA
BspLU11I 313 ACATGT A^CATGT
753 ACATGT
BsrDI 5816 CATTGC GCAATG(2/0)
3370 GCAATG
BstEII 3250 GGTGACC G^GT[ACGT]ACC
3904 GGTGACC
BstPI 3250 GGTGACC G^GT[ACGT]ACC
3904 GGTGACC
EcoO65I 3250 GGTGACC G^GT[ACGT]ACC
3904 GGTGACC
HincII 915 GTCAAC GT[CT]^[GA]AC
3272 GTCAAC
HindII 915 GTCAAC GT[CT]^[GA]AC
3272 GTCAAC
HindIII 2246 AAGCTT A^AGCTT
2694 AAGCTT
NciI 3744 CCGGG CC^[GC]GG
5444 CCCGG
RcaI 1386 TCATGA T^CATGA
5684 TCATGA
SapI 3835 GAAGAGC GCTCTTC(1/4)
5155 GAAGAGC
StuI 3437 AGGCCT AGG^CCT
4158 AGGCCT
XcmI 4416 CCAGGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5231 CCAGTGACAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII