Restriction map for
pF1KSDB0052
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1843 GACGTC GACGT^C
AccII 873 CGCG CG^CG
AccIII 1754 TCCGGA T^CCGGA
AcsI 824 GAATTC [GA]^AATT[CT]
ApoI 824 GAATTC [GA]^AATT[CT]
AvaI 1767 CTCGGG C^[CT]CG[GA]G
AviII 1597 TGCGCA TGC^GCA
BalI 1310 TGGCCA TGG^CCA
BbsI 1203 GAAGAC GAAGAC(2/6)
BcgI 285 CGAGTTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 939 CCGGG CC^[GC]GG
BpuAI 1203 GAAGAC GAAGAC(2/6)
BsaBI 1365 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1754 TCCGGA [AT]^CCGG[AT]
BseAI 1754 TCCGGA T^CCGGA
BsmBI 1764 CGTCTC CGTCTC(1/5)
BsmI 1258 GCATTC GAATGC(1/-1)
BsoBI 1767 CTCGGG C^[CT]CG[GA]G
BspEI 1754 TCCGGA T^CCGGA
BspHI 1047 TCATGA T^CATGA
BsrBI 1005 CCGCTC CCGCTC(-3/-3)
BsrDI 1557 CATTGC GCAATG(2/0)
BsrFI 301 ACCGGC [GA]^CCGG[CT]
BstUI 873 CGCG CG^CG
CfoI 1598 GCGC GCG^C
Cfr10I 301 ACCGGC [GA]^CCGG[CT]
DraIII 1303 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1546 GACTTTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1310 TGGCCA [CT]^GGCC[GA]
EcoNI 381 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 824 GAATTC G^AATTC
FspI 1597 TGCGCA TGC^GCA
HhaI 1598 GCGC GCG^C
HinP1I 1598 GCGC G^CGC
MamI 1365 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1310 TGGCCA TGG^CCA
MroI 1754 TCCGGA T^CCGGA
MscI 1310 TGGCCA TGG^CCA
MvnI 873 CGCG CG^CG
NciI 939 CCGGG CC^[GC]GG
NcoI 653 CCATGG C^CATGG
PvuII 337 CAGCTG CAG^CTG
RcaI 1047 TCATGA T^CATGA
SacI 103 GAGCTC GAGCT^C
SapI 1328 GAAGAGC GCTCTTC(1/4)
SbfI 1104 CCTGCAGG CCTGCA^GG
ScaI 326 AGTACT AGT^ACT
SmlI 233 CTCAAG C^T[CT][GA]AG
Sse8387I 1104 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1762 GGCGTC G[GA]^CG[CT]C
1843 GACGTC
AocI 18 CCTGAGG CC^T[ACGT]AGG
1641 CCTGAGG
AspHI 103 GAGCTC G[AT]GC[AT]^C
1012 GTGCTC
BanII 103 GAGCTC G[GA]GC[CT]^C
406 GGGCTC
BfaI 907 CTAG C^TAG
1292 CTAG
BsaHI 1762 GGCGTC G[GA]^CG[CT]C
1843 GACGTC
BseRI 385 GAGGAG GAGGAG(10/8)
1497 GAGGAG
BsiHKAI 103 GAGCTC G[AT]GC[AT]^C
1012 GTGCTC
BstYI 852 AGATCC [GA]^GATC[CT]
1211 AGATCC
Bsu36I 18 CCTGAGG CC^T[ACGT]AGG
1641 CCTGAGG
DsaI 653 CCATGG C^C[GA][CT]GG
1850 CCACGG
Eco81I 18 CCTGAGG CC^T[ACGT]AGG
1641 CCTGAGG
Hin1I 1762 GGCGTC G[GA]^CG[CT]C
1843 GACGTC
MaeI 907 CTAG C^TAG
1292 CTAG
MaeII 1535 ACGT A^CGT
1844 ACGT
MflI 852 AGATCC [GA]^GATC[CT]
1211 AGATCC
MseI 1222 TTAA T^TAA
1712 TTAA
MspA1I 337 CAGCTG C[AC]G^C[GT]G
697 CCGCTG
PflMI 1302 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1830 CCAGAAGCTGG
PstI 369 CTGCAG CTGCA^G
1105 CTGCAG
SexAI 801 ACCTGGT A^CC[AT]GGT
1304 ACCTGGT
Tru9I 1222 TTAA T^TAA
1712 TTAA
Tsp509I 825 AATT ^AATT
1345 AATT
Van91I 1302 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1830 CCAGAAGCTGG
XcmI 168 CCAGGTCAACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
524 CCAAGGACAAGCTGG
XhoII 852 AGATCC [GA]^GATC[CT]
1211 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BmyI BpmI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TfiI TseI Tsp45I
TspRI