Restriction map for
pF1KSDB0055
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccII AccIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
AvaI AviII AvrII BbeI BbrPI
BcgI BciVI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BstBI BstEII BstPI
BstUI CfoI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FspI HaeII HhaI HinP1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 26 enzymes) #
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Acc65I 3468 GGTACC G^GTACC
AclI 3407 AACGTT AA^CGTT
AflII 2770 CTTAAG C^TTAAG
Alw44I 1239 GTGCAC G^TGCAC
ApaLI 1239 GTGCAC G^TGCAC
Asp718I 3468 GGTACC G^GTACC
BbvCI 2847 CCTCAGC CCTCAGC(-5/-2)
BfrI 2770 CTTAAG C^TTAAG
BsaWI 1133 ACCGGA [AT]^CCGG[AT]
BseRI 1436 CTCCTC GAGGAG(10/8)
BsmBI 269 GAGACG CGTCTC(1/5)
BsrBI 3601 GAGCGG CCGCTC(-3/-3)
BsrDI 2967 CATTGC GCAATG(2/0)
BstAPI 2982 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI 3840 GAATTC G^AATTC
FseI 2022 GGCCGGCC GGCCGG^CC
HgaI 1912 GCGTC GACGC(5/10)
KpnI 3468 GGTACC GGTAC^C
NaeI 2023 GCCGGC GCC^GGC
NgoMIV 2023 GCCGGC G^CCGGC
PshAI 1876 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3407 AACGTT AA^CGTT
SacI 3427 GAGCTC GAGCT^C
ScaI 2867 AGTACT AGT^ACT
SspI 2636 AATATT AAT^ATT
XbaI 1323 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 33 enzymes) #
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AatII 271 GACGTC GACGT^C
1534 GACGTC
AcsI 3815 AAATTT [GA]^AATT[CT]
3840 GAATTC
AcyI 271 GACGTC G[GA]^CG[CT]C
1534 GACGTC
AflIII 2999 ACATGT A^C[GA][CT]GT
3070 ACATGT
ApoI 3815 AAATTT [GA]^AATT[CT]
3840 GAATTC
BamHI 2427 GGATCC G^GATCC
2736 GGATCC
BclI 2268 TGATCA T^GATCA
2673 TGATCA
BcnI 1571 CCCGG CC^[GC]GG
3852 CCCGG
BglI 3009 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3442 GCCCAGCAGGC
BlpI 3934 GCTGAGC GC^T[ACGT]AGC
3981 GCTGAGC
Bpu1102I 3934 GCTGAGC GC^T[ACGT]AGC
3981 GCTGAGC
BsaHI 271 GACGTC G[GA]^CG[CT]C
1534 GACGTC
BsgI 2393 CTGCAC GTGCAG(16/14)
3676 GTGCAG
BspLU11I 2999 ACATGT A^CATGT
3070 ACATGT
BspMI 1975 ACCTGC ACCTGC(4/8)
3451 GCAGGT
BsrFI 1070 ACCGGC [GA]^CCGG[CT]
2023 GCCGGC
BssSI 2575 CACGAG CACGAG(-5/-1)
3043 CACGAG
Bst1107I 3286 GTATAC GTA^TAC
3706 GTATAC
BstXI 3197 CCATGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3811 CCAGAAATTTGG
BstZ17I 3286 GTATAC GTA^TAC
3706 GTATAC
CelII 3934 GCTGAGC GC^T[ACGT]AGC
3981 GCTGAGC
Cfr10I 1070 ACCGGC [GA]^CCGG[CT]
2023 GCCGGC
DraIII 1264 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3662 CACTGAGTG
EcoRV 2959 GATATC GAT^ATC
3564 GATATC
EcoT22I 550 ATGCAT ATGCA^T
2229 ATGCAT
FbaI 2268 TGATCA T^GATCA
2673 TGATCA
Hin1I 271 GACGTC G[GA]^CG[CT]C
1534 GACGTC
HindIII 2116 AAGCTT A^AGCTT
3272 AAGCTT
NciI 1571 CCCGG CC^[GC]GG
3852 CCCGG
NsiI 550 ATGCAT ATGCA^T
2229 ATGCAT
SapI 1026 GAAGAGC GCTCTTC(1/4)
1618 GAAGAGC
TaqI 2163 TCGA T^CGA
3423 TCGA
TthHB8I 2163 TCGA T^CGA
3423 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI Asp700I AspHI AspI
AvaII BalI BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I Van91I XcmI XhoII
XmnI