Restriction map for
pF1KSDB0058
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# NO CUT ENZYMES ( 78 enzymes) #
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AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AviII AvrII BbrPI
BcgI BciVI BfrI BlnI BsaAI
BsiEI BsiWI BsmI BspDI BspLU11I
BssHII BstBI ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KspI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 2072 GGTACC G^GTACC
AcsI 536 AAATTC [GA]^AATT[CT]
AflIII 2239 ACGTGT A^C[GA][CT]GT
ApoI 536 AAATTC [GA]^AATT[CT]
Asp700I 78 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2072 GGTACC G^GTACC
BamHI 1196 GGATCC G^GATCC
BfaI 883 CTAG C^TAG
BglI 2293 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2406 AGATCT A^GATCT
BlpI 150 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 150 GCTCAGC GC^T[ACGT]AGC
BsgI 2959 GTGCAG GTGCAG(16/14)
Bsp1407I 860 TGTACA T^GTACA
BsrBI 1725 CCGCTC CCGCTC(-3/-3)
BsrDI 756 CATTGC GCAATG(2/0)
BsrFI 1262 ACCGGC [GA]^CCGG[CT]
BsrGI 860 TGTACA T^GTACA
Bst1107I 2398 GTATAC GTA^TAC
BstAPI 1527 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2920 GGTCACC G^GT[ACGT]ACC
BstPI 2920 GGTCACC G^GT[ACGT]ACC
BstZ17I 2398 GTATAC GTA^TAC
CelII 150 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1262 ACCGGC [GA]^CCGG[CT]
DraIII 2923 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2477 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2920 GGTCACC G^GT[ACGT]ACC
KpnI 2072 GGTACC GGTAC^C
MaeI 883 CTAG C^TAG
MseI 1901 TTAA T^TAA
NdeI 3275 CATATG CA^TATG
PaeR7I 598 CTCGAG C^TCGAG
SexAI 2756 ACCTGGT A^CC[AT]GGT
SspBI 860 TGTACA T^GTACA
Tru9I 1901 TTAA T^TAA
XcmI 1238 CCATTGTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 598 CTCGAG C^TCGAG
XmnI 78 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 2562 CGCG CG^CG
2741 CGCG
AocI 484 CCTGAGG CC^T[ACGT]AGG
1804 CCTGAGG
ApaI 119 GGGCCC GGGCC^C
1095 GGGCCC
BbvCI 65 CCTCAGC CCTCAGC(-5/-2)
346 GCTGAGG
BsmBI 2709 CGTCTC CGTCTC(1/5)
1562 GAGACG
BspHI 977 TCATGA T^CATGA
2519 TCATGA
BstUI 2562 CGCG CG^CG
2741 CGCG
BstXI 1206 CCATGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2084 CCACATTCATGG
Bsu36I 484 CCTGAGG CC^T[ACGT]AGG
1804 CCTGAGG
Eco81I 484 CCTGAGG CC^T[ACGT]AGG
1804 CCTGAGG
HincII 220 GTCAAC GT[CT]^[GA]AC
892 GTCAAC
HindII 220 GTCAAC GT[CT]^[GA]AC
892 GTCAAC
MvnI 2562 CGCG CG^CG
2741 CGCG
PflMI 156 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1496 CCATTGTGTGG
PpuMI 2612 GGGTCCT [GA]G^G[AT]CC[CT]
3121 GGGTCCT
PstI 130 CTGCAG CTGCA^G
2312 CTGCAG
PvuII 181 CAGCTG CAG^CTG
2690 CAGCTG
RcaI 977 TCATGA T^CATGA
2519 TCATGA
SacI 2005 GAGCTC GAGCT^C
2551 GAGCTC
ScaI 917 AGTACT AGT^ACT
2651 AGTACT
SfcI 130 CTGCAG C^T[GA][CT]AG
2312 CTGCAG
SmaI 1517 CCCGGG CCC^GGG
2076 CCCGGG
SmlI 304 CTCAAG C^T[CT][GA]AG
598 CTCGAG
Van91I 156 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1496 CCATTGTGTGG
XmaCI 1517 CCCGGG C^CCGGG
2076 CCCGGG
XmaI 1517 CCCGGG C^CCGGG
2076 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccIII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AspI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BclI BcnI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SphI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII