Restriction map for
pF1KSDB1426
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbrPI
BcgI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoT22I FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SspI
StuI SwaI Tru9I Tsp509I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1660 GACGTC GACGT^C
AflIII 380 ACATGT A^C[GA][CT]GT
AocI 1317 CCTCAGG CC^T[ACGT]AGG
Aor51HI 569 AGCGCT AGC^GCT
ApaI 540 GGGCCC GGGCC^C
AspI 1471 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 908 TGGCCA TGG^CCA
BamHI 1048 GGATCC G^GATCC
BbeI 143 GGCGCC GGCGC^C
BbvCI 432 CCTCAGC CCTCAGC(-5/-2)
BciVI 972 GGATAC GTATCC(6/5)
BclI 1361 TGATCA T^GATCA
BglII 32 AGATCT A^GATCT
BlpI 704 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 704 GCTCAGC GC^T[ACGT]AGC
BsaAI 1228 TACGTG [CT]AC^GT[GA]
BsgI 184 GTGCAG GTGCAG(16/14)
BsiEI 874 CGACCG CG[GA][CT]^CG
BsmBI 1476 CGTCTC CGTCTC(1/5)
Bsp1407I 1400 TGTACA T^GTACA
BspLU11I 380 ACATGT A^CATGT
BsrBI 1906 GAGCGG CCGCTC(-3/-3)
BsrGI 1400 TGTACA T^GTACA
BstAPI 1256 GCATCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1201 GGTGACC G^GT[ACGT]ACC
BstPI 1201 GGTGACC G^GT[ACGT]ACC
Bsu36I 1317 CCTCAGG CC^T[ACGT]AGG
CelII 704 GCTCAGC GC^T[ACGT]AGC
DraIII 1512 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 569 AGCGCT AGC^GCT
Eco81I 1317 CCTCAGG CC^T[ACGT]AGG
EcoNI 1095 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1201 GGTGACC G^GT[ACGT]ACC
EcoRV 1747 GATATC GAT^ATC
FbaI 1361 TGATCA T^GATCA
KasI 143 GGCGCC G^GCGCC
MluNI 908 TGGCCA TGG^CCA
MscI 908 TGGCCA TGG^CCA
NaeI 320 GCCGGC GCC^GGC
NarI 143 GGCGCC GG^CGCC
NgoMIV 320 GCCGGC G^CCGGC
NruI 169 TCGCGA TCG^CGA
PflFI 1471 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 936 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1344 GCTCTTC GCTCTTC(1/4)
SbfI 1449 CCTGCAGG CCTGCA^GG
SexAI 1412 ACCTGGT A^CC[AT]GGT
SfoI 143 GGCGCC GGC^GCC
SphI 1551 GCATGC GCATG^C
Sse8387I 1449 CCTGCAGG CCTGCA^GG
SspBI 1400 TGTACA T^GTACA
Tth111I 1471 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 936 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 170 CGCG CG^CG
222 CGCG
AcyI 143 GGCGCC G[GA]^CG[CT]C
1660 GACGTC
BbsI 201 GAAGAC GAAGAC(2/6)
1035 GTCTTC
BfaI 740 CTAG C^TAG
1957 CTAG
BglI 507 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
695 GCCTGGTGGGC
BpuAI 201 GAAGAC GAAGAC(2/6)
1035 GTCTTC
BsaHI 143 GGCGCC G[GA]^CG[CT]C
1660 GACGTC
BsaI 1754 GGTCTC GGTCTC(1/5)
1878 GGTCTC
BstUI 170 CGCG CG^CG
222 CGCG
BstYI 32 AGATCT [GA]^GATC[CT]
1048 GGATCC
EarI 1345 CTCTTC CTCTTC(1/4)
1830 GAAGAG
EcoT14I 911 CCAAGG C^C[AT][AT]GG
1118 CCAAGG
Hin1I 143 GGCGCC G[GA]^CG[CT]C
1660 GACGTC
Ksp632I 1345 CTCTTC CTCTTC(1/4)
1830 GAAGAG
MaeI 740 CTAG C^TAG
1957 CTAG
MflI 32 AGATCT [GA]^GATC[CT]
1048 GGATCC
MvnI 170 CGCG CG^CG
222 CGCG
PleI 1588 GACTC GAGTC(4/5)
1810 GACTC
PvuII 435 CAGCTG CAG^CTG
580 CAGCTG
SacI 600 GAGCTC GAGCT^C
1951 GAGCTC
SmaI 752 CCCGGG CCC^GGG
1271 CCCGGG
StyI 911 CCAAGG C^C[AT][AT]GG
1118 CCAAGG
TaqI 77 TCGA T^CGA
1607 TCGA
TfiI 173 GAATC G^A[AT]TC
1637 GATTC
TthHB8I 77 TCGA T^CGA
1607 TCGA
XhoII 32 AGATCT [GA]^GATC[CT]
1048 GGATCC
XmaCI 752 CCCGGG C^CCGGG
1271 CCCGGG
XmaI 752 CCCGGG C^CCGGG
1271 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I TspRI XcmI