Restriction map for
pF1KSDB1480
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# NO CUT ENZYMES ( 76 enzymes) #
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Acc65I AclI AcsI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbvCI
BfrI BlnI BsiWI BsmBI BsmI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CpoI DraI DraIII
Eco47III Eco81I EcoRI EcoRV FseI
FspI HindIII HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 2827 GACGTC GACGT^C
AccI 1069 GTCTAC GT^[AC][GT]AC
AccIII 1048 TCCGGA T^CCGGA
AflIII 188 ACGTGT A^C[GA][CT]GT
AhdI 2827 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 83 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2827 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1063 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 259 CACGTG CAC^GTG
BclI 2087 TGATCA T^GATCA
BlpI 91 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 91 GCTGAGC GC^T[ACGT]AGC
BseAI 1048 TCCGGA T^CCGGA
BsiEI 1643 CGGCCG CG[GA][CT]^CG
Bsp1407I 1134 TGTACA T^GTACA
BspDI 1789 ATCGAT AT^CGAT
BspEI 1048 TCCGGA T^CCGGA
BspHI 467 TCATGA T^CATGA
BsrBI 2470 GAGCGG CCGCTC(-3/-3)
BsrGI 1134 TGTACA T^GTACA
BstEII 1476 GGTCACC G^GT[ACGT]ACC
BstPI 1476 GGTCACC G^GT[ACGT]ACC
CelII 91 GCTGAGC GC^T[ACGT]AGC
ClaI 1789 ATCGAT AT^CGAT
DrdI 1959 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1643 CGGCCG C^GGCCG
Eam1105I 2827 GACGTCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1643 CGGCCG C^GGCCG
Eco52I 1643 CGGCCG C^GGCCG
EcoNI 115 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1476 GGTCACC G^GT[ACGT]ACC
EcoT22I 1324 ATGCAT ATGCA^T
FbaI 2087 TGATCA T^GATCA
MroI 1048 TCCGGA T^CCGGA
MseI 2744 TTAA T^TAA
NsiI 1324 ATGCAT ATGCA^T
PflFI 1063 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 259 CACGTG CAC^GTG
PmlI 259 CACGTG CAC^GTG
PshAI 500 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 467 TCATGA T^CATGA
SacI 1429 GAGCTC GAGCT^C
ScaI 1415 AGTACT AGT^ACT
SmaI 665 CCCGGG CCC^GGG
SmlI 1963 CTCAAG C^T[CT][GA]AG
SphI 2174 GCATGC GCATG^C
SspBI 1134 TGTACA T^GTACA
Tru9I 2744 TTAA T^TAA
Tth111I 1063 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2310 TCTAGA T^CTAGA
XmaCI 665 CCCGGG C^CCGGG
XmaI 665 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BciVI 414 GGATAC GTATCC(6/5)
2909 GTATCC
BglII 1781 AGATCT A^GATCT
1940 AGATCT
BsaAI 171 CACGTA [CT]AC^GT[GA]
259 CACGTG
BsaI 325 GAGACC GGTCTC(1/5)
1009 GAGACC
BsaWI 1048 TCCGGA [AT]^CCGG[AT]
2399 ACCGGA
BseRI 1638 CTCCTC GAGGAG(10/8)
2688 CTCCTC
BstAPI 797 GCACCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1115 GCACCCGCTGC
EarI 130 GAAGAG CTCTTC(1/4)
691 GAAGAG
HgaI 1959 GACGC GACGC(5/10)
464 GCGTC
HincII 2260 GTCAAC GT[CT]^[GA]AC
2629 GTCAAC
HindII 2260 GTCAAC GT[CT]^[GA]AC
2629 GTCAAC
Ksp632I 130 GAAGAG CTCTTC(1/4)
691 GAAGAG
NaeI 1498 GCCGGC GCC^GGC
2809 GCCGGC
NgoMIV 1498 GCCGGC G^CCGGC
2809 GCCGGC
PflMI 2163 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2366 CCATCCGCTGG
PpuMI 1061 AGGACCT [GA]G^G[AT]CC[CT]
1808 AGGACCC
SexAI 176 ACCAGGT A^CC[AT]GGT
2270 ACCTGGT
StuI 851 AGGCCT AGG^CCT
1158 AGGCCT
TfiI 396 GAATC G^A[AT]TC
2940 GATTC
Van91I 2163 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2366 CCATCCGCTGG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII