################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BciVI BfaI BfrI BlnI BlpI Bpu1102I BspDI BspLU11I BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRV EcoT22I FseI HindIII HpaI KpnI KspI MaeI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI SacII SalI SbfI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflIII 2618 ACACGT A^C[GA][CT]GT BbrPI 787 CACGTG CAC^GTG BbsI 1804 GTCTTC GAAGAC(2/6) BbvCI 2670 GCTGAGG CCTCAGC(-5/-2) BglII 2204 AGATCT A^GATCT BpuAI 1804 GTCTTC GAAGAC(2/6) BsaBI 2994 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 2681 CGTACG C^GTACG BsmBI 3583 GAGACG CGTCTC(1/5) BsmI 2826 GCATTC GAATGC(1/-1) BspHI 2873 TCATGA T^CATGA BsrFI 170 GCCGGC [GA]^CCGG[CT] BstAPI 1874 GCACTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 655 GGTGACC G^GT[ACGT]ACC BstPI 655 GGTGACC G^GT[ACGT]ACC Cfr10I 170 GCCGGC [GA]^CCGG[CT] CpoI 2042 CGGTCCG CG^G[AT]CCG EagI 2685 CGGCCG C^GGCCG EclXI 2685 CGGCCG C^GGCCG Eco52I 2685 CGGCCG C^GGCCG EcoO65I 655 GGTGACC G^GT[ACGT]ACC EcoRI 3379 GAATTC G^AATTC MamI 2994 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 2264 TTAA T^TAA NaeI 170 GCCGGC GCC^GGC NgoMIV 170 GCCGGC G^CCGGC PmaCI 787 CACGTG CAC^GTG PmlI 787 CACGTG CAC^GTG RcaI 2873 TCATGA T^CATGA RsrII 2042 CGGTCCG CG^G[AT]CCG SapI 2883 GCTCTTC GCTCTTC(1/4) ScaI 1178 AGTACT AGT^ACT SexAI 737 ACCAGGT A^CC[AT]GGT SfiI 2397 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 995 GCATGC GCATG^C Tru9I 2264 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 1417 GACGTC GACGT^C 3585 GACGTC AccI 1168 GTCTAC GT^[AC][GT]AC 2743 GTCTAC AccIII 2945 TCCGGA T^CCGGA 3383 TCCGGA Alw44I 75 GTGCAC G^TGCAC 1158 GTGCAC AlwNI 2384 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 3244 CAGATCCTG ApaLI 75 GTGCAC G^TGCAC 1158 GTGCAC BamHI 2159 GGATCC G^GATCC 3272 GGATCC BcgI 393 CGAAGGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2334 CGACGGGCCTGC BclI 1091 TGATCA T^GATCA 1478 TGATCA BsaAI 787 CACGTG [CT]AC^GT[GA] 1171 TACGTG BsaI 1327 GAGACC GGTCTC(1/5) 2659 GAGACC BsaWI 2945 TCCGGA [AT]^CCGG[AT] 3383 TCCGGA BseAI 2945 TCCGGA T^CCGGA 3383 TCCGGA BspEI 2945 TCCGGA T^CCGGA 3383 TCCGGA BssSI 292 CTCGTG CACGAG(-5/-1) 1799 CTCGTG FbaI 1091 TGATCA T^GATCA 1478 TGATCA HincII 1132 GTCAAC GT[CT]^[GA]AC 2518 GTCAAC HindII 1132 GTCAAC GT[CT]^[GA]AC 2518 GTCAAC MroI 2945 TCCGGA T^CCGGA 3383 TCCGGA NspI 995 GCATGC [GA]CATG^[CT] 1727 GCATGT PaeR7I 2134 CTCGAG C^TCGAG 3462 CTCGAG PflMI 786 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3573 CCACAGCCTGG PvuII 3027 CAGCTG CAG^CTG 3043 CAGCTG Van91I 786 CCACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3573 CCACAGCCTGG XhoI 2134 CTCGAG C^TCGAG 3462 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI