Restriction map for
pF1KSDB1510
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 589 GGTACC G^GTACC
AccI 877 GTCGAC GT^[AC][GT]AC
Alw44I 596 GTGCAC G^TGCAC
Aor51HI 45 AGCGCT AGC^GCT
ApaLI 596 GTGCAC G^TGCAC
Asp700I 1540 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 589 GGTACC G^GTACC
BanI 589 GGTACC G^G[CT][GA]CC
BlpI 1405 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1405 GCTCAGC GC^T[ACGT]AGC
BsaAI 1210 TACGTG [CT]AC^GT[GA]
BsgI 1415 CTGCAC GTGCAG(16/14)
BsrDI 530 CATTGC GCAATG(2/0)
BsrFI 353 GCCGGC [GA]^CCGG[CT]
BssSI 432 CACGAG CACGAG(-5/-1)
BstXI 1610 CCACTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 380 AGATCC [GA]^GATC[CT]
CelII 1405 GCTCAGC GC^T[ACGT]AGC
Cfr10I 353 GCCGGC [GA]^CCGG[CT]
EarI 846 CTCTTC CTCTTC(1/4)
Eco47III 45 AGCGCT AGC^GCT
EcoNI 327 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 877 GTCGAC GT[CT]^[GA]AC
HindII 877 GTCGAC GT[CT]^[GA]AC
KpnI 589 GGTACC GGTAC^C
Ksp632I 846 CTCTTC CTCTTC(1/4)
MflI 380 AGATCC [GA]^GATC[CT]
NaeI 353 GCCGGC GCC^GGC
NcoI 927 CCATGG C^CATGG
NgoMIV 353 GCCGGC G^CCGGC
PleI 1426 GAGTC GAGTC(4/5)
PpuMI 789 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 620 GAGCTC GAGCT^C
SalI 877 GTCGAC G^TCGAC
SfiI 280 GGCCCTTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 918 AGGCCT AGG^CCT
TfiI 1560 GAATC G^A[AT]TC
Tsp509I 390 AATT ^AATT
XhoII 380 AGATCC [GA]^GATC[CT]
XmnI 1540 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 381 GATCC GGATC(4/5)
1215 GGATC
BalI 647 TGGCCA TGG^CCA
1619 TGGCCA
BglI 223 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
281 GCCCTTGCGGC
BsmBI 196 CGTCTC CGTCTC(1/5)
435 GAGACG
BsmFI 724 GTCCC GGGAC(10/14)
791 GTCCC
BspMI 1413 ACCTGC ACCTGC(4/8)
676 GCAGGT
BstF5I 822 CATCC GGATG(2/0)
1101 CATCC
DdeI 1406 CTCAG C^T[ACGT]AG
1525 CTAAG
FokI 822 CATCC GGATG(9/13)
1101 CATCC
HaeII 15 GGCGCT [GA]GCGC^[CT]
45 AGCGCT
HinfI 1426 GAGTC G^A[ACGT]TC
1560 GAATC
KspI 24 CCGCGG CCGC^GG
123 CCGCGG
MaeII 1211 ACGT A^CGT
1442 ACGT
MaeIII 550 GTGAC ^GT[ACGT]AC
766 GTCAC
MluNI 647 TGGCCA TGG^CCA
1619 TGGCCA
MscI 647 TGGCCA TGG^CCA
1619 TGGCCA
MslI 651 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1101 CATCCTTGTG
SacII 24 CCGCGG CCGC^GG
123 CCGCGG
Tsp45I 550 GTGAC ^GT[GC]AC
766 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanII
BbvI BcgI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI TspRI TthHB8I
Van91I