Restriction map for
pF1KSDB2506
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BpmI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
MaeI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwI 873 GATCC GGATC(4/5)
AlwNI 834 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1102 AGCGCT AGC^GCT
ApaI 465 GGGCCC GGGCC^C
AvaI 101 CCCGAG C^[CT]CG[GA]G
BalI 815 TGGCCA TGG^CCA
BanI 196 GGCGCC G^G[CT][GA]CC
BbeI 196 GGCGCC GGCGC^C
BcnI 188 CCGGG CC^[GC]GG
BlpI 420 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 420 GCTGAGC GC^T[ACGT]AGC
BsaAI 133 TACGTG [CT]AC^GT[GA]
BsaBI 219 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 355 GAGACC GGTCTC(1/5)
BsmBI 949 GAGACG CGTCTC(1/5)
BsoBI 101 CCCGAG C^[CT]CG[GA]G
BsrDI 770 GCAATG GCAATG(2/0)
BsrI 1076 CCAGT ACTGG(1/-1)
BstEII 636 GGTCACC G^GT[ACGT]ACC
BstPI 636 GGTCACC G^GT[ACGT]ACC
BstXI 818 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 872 AGATCC [GA]^GATC[CT]
CelII 420 GCTGAGC GC^T[ACGT]AGC
Eco47III 1102 AGCGCT AGC^GCT
EcoO65I 636 GGTCACC G^GT[ACGT]ACC
HapII 188 CCGG C^CGG
HpaII 188 CCGG C^CGG
KasI 196 GGCGCC G^GCGCC
MamI 219 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 872 AGATCC [GA]^GATC[CT]
MluNI 815 TGGCCA TGG^CCA
MscI 815 TGGCCA TGG^CCA
MspI 188 CCGG C^CGG
NarI 196 GGCGCC GG^CGCC
NciI 188 CCGGG CC^[GC]GG
PflMI 833 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 304 GGGACCC [GA]G^G[AT]CC[CT]
SacI 29 GAGCTC GAGCT^C
SbfI 897 CCTGCAGG CCTGCA^GG
SexAI 35 ACCAGGT A^CC[AT]GGT
SfoI 196 GGCGCC GGC^GCC
Sse8387I 897 CCTGCAGG CCTGCA^GG
StuI 1001 AGGCCT AGG^CCT
Van91I 833 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 872 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbsI 1196 GAAGAC GAAGAC(2/6)
844 GTCTTC
BclI 65 TGATCA T^GATCA
203 TGATCA
BglI 272 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
467 GCCCCGCGGGC
BpuAI 1196 GAAGAC GAAGAC(2/6)
844 GTCTTC
BsgI 879 CTGCAC GTGCAG(16/14)
970 GTGCAG
BsmAI 355 GAGAC GTCTC(1/5)
949 GAGAC
DraIII 34 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
660 CACTCTGTG
FbaI 65 TGATCA T^GATCA
203 TGATCA
HgaI 154 GACGC GACGC(5/10)
389 GCGTC
KspI 470 CCGCGG CCGC^GG
979 CCGCGG
NcoI 590 CCATGG C^CATGG
1010 CCATGG
PstI 781 CTGCAG CTGCA^G
898 CTGCAG
PvuII 481 CAGCTG CAG^CTG
973 CAGCTG
SacII 470 CCGCGG CCGC^GG
979 CCGCGG
SapI 267 GAAGAGC GCTCTTC(1/4)
57 GCTCTTC
SfcI 781 CTGCAG C^T[GA][CT]AG
898 CTGCAG
TaqI 923 TCGA T^CGA
959 TCGA
TthHB8I 923 TCGA T^CGA
959 TCGA
XcmI 1052 CCAACGGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1076 CCAGTGGCAGCTTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AspHI AvaII BanII BbvI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I TspRI