Restriction map for
pF1KSDF0018
##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BbeI BcgI BciVI BfrI BglII
BlnI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HindIII KasI KspI MfeI MluI
MunI NarI NdeI NheI NotI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3322 GGTACC G^GTACC
AccI 2747 GTCTAC GT^[AC][GT]AC
Asp718I 3322 GGTACC G^GTACC
BbsI 3756 GTCTTC GAAGAC(2/6)
BbvCI 2107 GCTGAGG CCTCAGC(-5/-2)
BclI 2919 TGATCA T^GATCA
BpuAI 3756 GTCTTC GAAGAC(2/6)
BsaBI 2377 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 1863 CATTGC GCAATG(2/0)
BssHII 871 GCGCGC G^CGCGC
BstAPI 1883 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3127 GGTCACC G^GT[ACGT]ACC
BstPI 3127 GGTCACC G^GT[ACGT]ACC
DraIII 87 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3127 GGTCACC G^GT[ACGT]ACC
FbaI 2919 TGATCA T^GATCA
HpaI 4015 GTTAAC GTT^AAC
KpnI 3322 GGTACC GGTAC^C
MamI 2377 GATTCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 4016 TTAA T^TAA
NruI 786 TCGCGA TCG^CGA
PaeR7I 1358 CTCGAG C^TCGAG
SphI 864 GCATGC GCATG^C
StuI 1659 AGGCCT AGG^CCT
Tru9I 4016 TTAA T^TAA
XbaI 3660 TCTAGA T^CTAGA
XcmI 631 CCAGCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1358 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 2300 TCCGGA T^CCGGA
3543 TCCGGA
AcyI 2439 GGCGTC G[GA]^CG[CT]C
3768 GACGCC
AocI 4 CCTGAGG CC^T[ACGT]AGG
1570 CCTGAGG
AspI 2436 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3202 GACCCTGTC
BalI 1037 TGGCCA TGG^CCA
2739 TGGCCA
BbrPI 2620 CACGTG CAC^GTG
4024 CACGTG
BsaAI 2620 CACGTG [CT]AC^GT[GA]
4024 CACGTG
BsaHI 2439 GGCGTC G[GA]^CG[CT]C
3768 GACGCC
BsaI 1115 GAGACC GGTCTC(1/5)
1346 GGTCTC
BseAI 2300 TCCGGA T^CCGGA
3543 TCCGGA
BspEI 2300 TCCGGA T^CCGGA
3543 TCCGGA
BspLU11I 2653 ACATGT A^CATGT
2751 ACATGT
BspMI 1166 ACCTGC ACCTGC(4/8)
429 GCAGGT
BstXI 1716 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3833 CCAGATACCTGG
Bsu36I 4 CCTGAGG CC^T[ACGT]AGG
1570 CCTGAGG
DsaI 125 CCATGG C^C[GA][CT]GG
3701 CCATGG
Eco81I 4 CCTGAGG CC^T[ACGT]AGG
1570 CCTGAGG
HgaI 3768 GACGC GACGC(5/10)
2440 GCGTC
Hin1I 2439 GGCGTC G[GA]^CG[CT]C
3768 GACGCC
HincII 3215 GTCAAC GT[CT]^[GA]AC
4015 GTTAAC
HindII 3215 GTCAAC GT[CT]^[GA]AC
4015 GTTAAC
MluNI 1037 TGGCCA TGG^CCA
2739 TGGCCA
MroI 2300 TCCGGA T^CCGGA
3543 TCCGGA
MscI 1037 TGGCCA TGG^CCA
2739 TGGCCA
NaeI 2534 GCCGGC GCC^GGC
2898 GCCGGC
NcoI 125 CCATGG C^CATGG
3701 CCATGG
NgoMIV 2534 GCCGGC G^CCGGC
2898 GCCGGC
PflFI 2436 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3202 GACCCTGTC
PmaCI 2620 CACGTG CAC^GTG
4024 CACGTG
PmlI 2620 CACGTG CAC^GTG
4024 CACGTG
SapI 3600 GAAGAGC GCTCTTC(1/4)
1008 GCTCTTC
SmaI 320 CCCGGG CCC^GGG
3911 CCCGGG
Tth111I 2436 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3202 GACCCTGTC
XmaCI 320 CCCGGG C^CCGGG
3911 CCCGGG
XmaI 320 CCCGGG C^CCGGG
3911 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BamHI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII