Restriction map for
pF1KSDF0070
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII AclI AcsI AflII AhdI
ApoI AscI AseI AsnI Asp700I
AspEI AspI BamHI BbvCI BclI
BfrI BsaBI BsaI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoNI EcoRI EcoT22I FbaI HindIII
HpaI MamI MluI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 445 GGTACC G^GTACC
AccI 2878 GTCTAC GT^[AC][GT]AC
Alw44I 721 GTGCAC G^TGCAC
AlwI 2410 GGATC GGATC(4/5)
AocI 1504 CCTGAGG CC^T[ACGT]AGG
ApaLI 721 GTGCAC G^TGCAC
Asp718I 445 GGTACC G^GTACC
AviII 1072 TGCGCA TGC^GCA
AvrII 630 CCTAGG C^CTAGG
BbrPI 1945 CACGTG CAC^GTG
BciVI 1375 GGATAC GTATCC(6/5)
BlnI 630 CCTAGG C^CTAGG
BlpI 2885 GCTCAGC GC^T[ACGT]AGC
BpmI 3411 CTGGAG CTGGAG(16/14)
Bpu1102I 2885 GCTCAGC GC^T[ACGT]AGC
BssSI 1965 CTCGTG CACGAG(-5/-1)
BstEII 1530 GGTGACC G^GT[ACGT]ACC
BstPI 1530 GGTGACC G^GT[ACGT]ACC
Bsu36I 1504 CCTGAGG CC^T[ACGT]AGG
CelII 2885 GCTCAGC GC^T[ACGT]AGC
Eco81I 1504 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1530 GGTGACC G^GT[ACGT]ACC
EcoRV 3757 GATATC GAT^ATC
FseI 128 GGCCGGCC GGCCGG^CC
FspI 1072 TGCGCA TGC^GCA
KpnI 445 GGTACC GGTAC^C
MfeI 1914 CAATTG C^AATTG
MseI 1040 TTAA T^TAA
MunI 1914 CAATTG C^AATTG
PmaCI 1945 CACGTG CAC^GTG
PmlI 1945 CACGTG CAC^GTG
SacI 735 GAGCTC GAGCT^C
SapI 1200 GAAGAGC GCTCTTC(1/4)
SmlI 223 CTCAAG C^T[CT][GA]AG
TfiI 3646 GATTC G^A[AT]TC
Tru9I 1040 TTAA T^TAA
XcmI 1740 CCAGAACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccIII 516 TCCGGA T^CCGGA
1476 TCCGGA
AflIII 2375 ACGTGT A^C[GA][CT]GT
3404 ACGTGT
AgeI 1545 ACCGGT A^CCGGT
1811 ACCGGT
BbsI 898 GAAGAC GAAGAC(2/6)
2101 GAAGAC
BcgI 373 CGAAACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2987 GCATGTTGTTCG
BfaI 631 CTAG C^TAG
1487 CTAG
BglII 644 AGATCT A^GATCT
1355 AGATCT
BpuAI 898 GAAGAC GAAGAC(2/6)
2101 GAAGAC
BsaAI 995 TACGTG [CT]AC^GT[GA]
1945 CACGTG
BseAI 516 TCCGGA T^CCGGA
1476 TCCGGA
BsmAI 1717 GAGAC GTCTC(1/5)
221 GTCTC
BsmI 1984 GAATGC GAATGC(1/-1)
3094 GAATGC
BspEI 516 TCCGGA T^CCGGA
1476 TCCGGA
BsrBI 139 GAGCGG CCGCTC(-3/-3)
3394 GAGCGG
BssHII 1307 GCGCGC G^CGCGC
3862 GCGCGC
HincII 2947 GTCAAC GT[CT]^[GA]AC
3289 GTCAAC
HindII 2947 GTCAAC GT[CT]^[GA]AC
3289 GTCAAC
MaeI 631 CTAG C^TAG
1487 CTAG
MroI 516 TCCGGA T^CCGGA
1476 TCCGGA
NcoI 1095 CCATGG C^CATGG
2634 CCATGG
NruI 370 TCGCGA TCG^CGA
2223 TCGCGA
NspI 2987 GCATGT [GA]CATG^[CT]
3434 GCATGT
PflMI 1327 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCATGCGCTGG
PinAI 1545 ACCGGT A^CCGGT
1811 ACCGGT
PshAI 241 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACGAGTGTC
PvuII 958 CAGCTG CAG^CTG
1364 CAGCTG
StuI 707 AGGCCT AGG^CCT
1298 AGGCCT
Tsp509I 1915 AATT ^AATT
3577 AATT
Van91I 1327 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1646 CCATGCGCTGG
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# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SgrAI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI