################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AclI AcsI AflIII AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI BsmI BspDI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MaeI MamI MfeI MflI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI StyI SwaI TfiI Tsp509I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 2147 GGTACC G^GTACC AccIII 2051 TCCGGA T^CCGGA AflII 507 CTTAAG C^TTAAG AlwI 1377 GATCC GGATC(4/5) AocI 817 CCTCAGG CC^T[ACGT]AGG Asp718I 2147 GGTACC G^GTACC AspI 1666 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 2367 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 507 CTTAAG C^TTAAG BsaAI 1519 TACGTG [CT]AC^GT[GA] BseAI 2051 TCCGGA T^CCGGA Bsp1407I 1076 TGTACA T^GTACA BspEI 2051 TCCGGA T^CCGGA BspHI 1373 TCATGA T^CATGA BsrGI 1076 TGTACA T^GTACA Bsu36I 817 CCTCAGG CC^T[ACGT]AGG Eco81I 817 CCTCAGG CC^T[ACGT]AGG HindIII 2308 AAGCTT A^AGCTT KpnI 2147 GGTACC GGTAC^C MroI 2051 TCCGGA T^CCGGA PaeR7I 2031 CTCGAG C^TCGAG PflFI 1666 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 547 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1246 CAGCTG CAG^CTG RcaI 1373 TCATGA T^CATGA ScaI 299 AGTACT AGT^ACT SphI 251 GCATGC GCATG^C SspBI 1076 TGTACA T^GTACA SspI 1207 AATATT AAT^ATT StuI 867 AGGCCT AGG^CCT Tth111I 1666 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2031 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AgeI 1649 ACCGGT A^CCGGT 2144 ACCGGT BbeI 1911 GGCGCC GGCGC^C 1987 GGCGCC BsaI 37 GAGACC GGTCTC(1/5) 2121 GGTCTC BsmAI 37 GAGAC GTCTC(1/5) 2122 GTCTC BsrBI 363 CCGCTC CCGCTC(-3/-3) 166 GAGCGG BsrDI 1764 CATTGC GCAATG(2/0) 2273 GCAATG BstAPI 974 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2380 GCACCCCCTGC HgaI 1173 GACGC GACGC(5/10) 1601 GCGTC KasI 1911 GGCGCC G^GCGCC 1987 GGCGCC MaeII 1358 ACGT A^CGT 1520 ACGT MseI 508 TTAA T^TAA 2299 TTAA NarI 1911 GGCGCC GG^CGCC 1987 GGCGCC PinAI 1649 ACCGGT A^CCGGT 2144 ACCGGT SacI 454 GAGCTC GAGCT^C 874 GAGCTC SapI 540 GAAGAGC GCTCTTC(1/4) 1821 GCTCTTC SbfI 669 CCTGCAGG CCTGCA^GG 1605 CCTGCAGG SfoI 1911 GGCGCC GGC^GCC 1987 GGCGCC Sse8387I 669 CCTGCAGG CCTGCA^GG 1605 CCTGCAGG TaqI 26 TCGA T^CGA 2032 TCGA Tru9I 508 TTAA T^TAA 2299 TTAA Tsp45I 175 GTGAC ^GT[GC]AC 1171 GTGAC TthHB8I 26 TCGA T^CGA 2032 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwNI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI TspRI Van91I