Restriction map for
pF1KSDF0261
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI MamI
MfeI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 3679 GACGTC GACGT^C
AccI 3787 GTCTAC GT^[AC][GT]AC
AflIII 4240 ACGCGT A^C[GA][CT]GT
Alw44I 4724 GTGCAC G^TGCAC
ApaLI 4724 GTGCAC G^TGCAC
Asp700I 4465 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2289 CCTAGG C^CTAGG
BbeI 4423 GGCGCC GGCGC^C
BbrPI 1649 CACGTG CAC^GTG
BbsI 3511 GAAGAC GAAGAC(2/6)
BlnI 2289 CCTAGG C^CTAGG
BpuAI 3511 GAAGAC GAAGAC(2/6)
BsaAI 1649 CACGTG [CT]AC^GT[GA]
BspHI 1283 TCATGA T^CATGA
BsrDI 2558 CATTGC GCAATG(2/0)
BsrFI 3107 GCCGGC [GA]^CCGG[CT]
BssSI 2237 CTCGTG CACGAG(-5/-1)
BstAPI 3111 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2945 GGTCACC G^GT[ACGT]ACC
BstPI 2945 GGTCACC G^GT[ACGT]ACC
Cfr10I 3107 GCCGGC [GA]^CCGG[CT]
EcoO65I 2945 GGTCACC G^GT[ACGT]ACC
EcoRI 55 GAATTC G^AATTC
HindIII 4468 AAGCTT A^AGCTT
KasI 4423 GGCGCC G^GCGCC
KspI 3379 CCGCGG CCGC^GG
MluI 4240 ACGCGT A^CGCGT
NaeI 3107 GCCGGC GCC^GGC
NarI 4423 GGCGCC GG^CGCC
NdeI 2605 CATATG CA^TATG
NgoMIV 3107 GCCGGC G^CCGGC
NspI 2304 GCATGC [GA]CATG^[CT]
PmaCI 1649 CACGTG CAC^GTG
PmlI 1649 CACGTG CAC^GTG
RcaI 1283 TCATGA T^CATGA
SacII 3379 CCGCGG CCGC^GG
SexAI 1853 ACCTGGT A^CC[AT]GGT
SfoI 4423 GGCGCC GGC^GCC
SphI 2304 GCATGC GCATG^C
SspI 4140 AATATT AAT^ATT
XmnI 4465 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 3380 CGCG CG^CG
4241 CGCG
AccIII 300 TCCGGA T^CCGGA
2826 TCCGGA
BamHI 1459 GGATCC G^GATCC
2159 GGATCC
BsaWI 300 TCCGGA [AT]^CCGG[AT]
2826 TCCGGA
BseAI 300 TCCGGA T^CCGGA
2826 TCCGGA
BsmBI 217 GAGACG CGTCTC(1/5)
3677 GAGACG
BspEI 300 TCCGGA T^CCGGA
2826 TCCGGA
BsrBI 1223 CCGCTC CCGCTC(-3/-3)
4147 CCGCTC
BstUI 3380 CGCG CG^CG
4241 CGCG
MroI 300 TCCGGA T^CCGGA
2826 TCCGGA
MseI 190 TTAA T^TAA
794 TTAA
MslI 2233 CATGCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3582 CATAGCTGTG
MvnI 3380 CGCG CG^CG
4241 CGCG
NcoI 2144 CCATGG C^CATGG
3434 CCATGG
SacI 367 GAGCTC GAGCT^C
2830 GAGCTC
SmaI 1297 CCCGGG CCC^GGG
3364 CCCGGG
TaqI 4554 TCGA T^CGA
4749 TCGA
Tru9I 190 TTAA T^TAA
794 TTAA
TthHB8I 4554 TCGA T^CGA
4749 TCGA
XbaI 399 TCTAGA T^CTAGA
2037 TCTAGA
XmaCI 1297 CCCGGG C^CCGGG
3364 CCCGGG
XmaI 1297 CCCGGG C^CCGGG
3364 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XcmI XhoII