Restriction map for
pF1KA0099
##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AspEI AviII AvrII BciVI BfrI
BglI BglII BlnI BsaBI BsaI
BseAI BsmBI BspEI BsrBI BssSI
Bst1107I BstEII BstPI BstZ17I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI HpaI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PmeI PpuMI PshAI
Psp1406I RsrII SacII SalI SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
StuI SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 871 GACGTC GACGT^C
AccI 1405 GTCTAC GT^[AC][GT]AC
AgeI 2438 ACCGGT A^CCGGT
Alw44I 3231 GTGCAC G^TGCAC
AocI 3091 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1297 AGCGCT AGC^GCT
ApaI 3454 GGGCCC GGGCC^C
ApaLI 3231 GTGCAC G^TGCAC
AseI 2776 ATTAAT AT^TAAT
AsnI 2776 ATTAAT AT^TAAT
BbeI 1966 GGCGCC GGCGC^C
BbrPI 2842 CACGTG CAC^GTG
BcgI 1955 GCAGTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 507 TGATCA T^GATCA
BsiWI 3199 CGTACG C^GTACG
BsmI 1704 GAATGC GAATGC(1/-1)
BspHI 387 TCATGA T^CATGA
BsrFI 2438 ACCGGT [GA]^CCGG[CT]
BssHII 3207 GCGCGC G^CGCGC
BstAPI 432 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2429 TTCGAA TT^CGAA
Bsu36I 3091 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2438 ACCGGT [GA]^CCGG[CT]
DraII 3453 AGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 1297 AGCGCT AGC^GCT
Eco81I 3091 CCTGAGG CC^T[ACGT]AGG
EcoNI 190 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3453 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1602 GAATTC G^AATTC
FbaI 507 TGATCA T^GATCA
HgaI 232 GACGC GACGC(5/10)
HindIII 2232 AAGCTT A^AGCTT
KasI 1966 GGCGCC G^GCGCC
NarI 1966 GGCGCC GG^CGCC
NspV 2429 TTCGAA TT^CGAA
PinAI 2438 ACCGGT A^CCGGT
PmaCI 2842 CACGTG CAC^GTG
PmlI 2842 CACGTG CAC^GTG
PshBI 2776 ATTAAT AT^TAAT
PvuI 631 CGATCG CGAT^CG
RcaI 387 TCATGA T^CATGA
SapI 416 GAAGAGC GCTCTTC(1/4)
ScaI 3425 AGTACT AGT^ACT
SexAI 579 ACCTGGT A^CC[AT]GGT
SfoI 1966 GGCGCC GGC^GCC
SfuI 2429 TTCGAA TT^CGAA
SmaI 687 CCCGGG CCC^GGG
SspI 495 AATATT AAT^ATT
XmaCI 687 CCCGGG C^CCGGG
XmaI 687 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1098 GGTACC G^GTACC
2441 GGTACC
AccII 1713 CGCG CG^CG
3208 CGCG
AcyI 871 GACGTC G[GA]^CG[CT]C
1966 GGCGCC
AflIII 1053 ACATGT A^C[GA][CT]GT
3069 ACATGT
Asp700I 2422 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2677 GAACGGATTC
Asp718I 1098 GGTACC G^GTACC
2441 GGTACC
AspI 578 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1622 GACAAGGTC
AvaI 687 CCCGGG C^[CT]CG[GA]G
2340 CCCGAG
BalI 2802 TGGCCA TGG^CCA
3410 TGGCCA
BbsI 575 GAAGAC GAAGAC(2/6)
2563 GTCTTC
BbvCI 1775 CCTCAGC CCTCAGC(-5/-2)
1804 CCTCAGC
BcnI 687 CCCGG CC^[GC]GG
688 CCGGG
BpuAI 575 GAAGAC GAAGAC(2/6)
2563 GTCTTC
BsaHI 871 GACGTC G[GA]^CG[CT]C
1966 GGCGCC
BsaWI 1653 TCCGGT [AT]^CCGG[AT]
2438 ACCGGT
BsiEI 631 CGATCG CG[GA][CT]^CG
3084 CGGTCG
BsmAI 518 GAGAC GTCTC(1/5)
2978 GTCTC
BsoBI 687 CCCGGG C^[CT]CG[GA]G
2340 CCCGAG
Bsp1407I 2868 TGTACA T^GTACA
3036 TGTACA
BspLU11I 1053 ACATGT A^CATGT
3069 ACATGT
BsrDI 3128 GCAATG GCAATG(2/0)
3164 GCAATG
BsrGI 2868 TGTACA T^GTACA
3036 TGTACA
BstUI 1713 CGCG CG^CG
3208 CGCG
EaeI 2802 TGGCCA [CT]^GGCC[GA]
3410 TGGCCA
EcoT22I 2856 ATGCAT ATGCA^T
3349 ATGCAT
Hin1I 871 GACGTC G[GA]^CG[CT]C
1966 GGCGCC
HincII 1194 GTTGAC GT[CT]^[GA]AC
3445 GTTGAC
HindII 1194 GTTGAC GT[CT]^[GA]AC
3445 GTTGAC
KpnI 1098 GGTACC GGTAC^C
2441 GGTACC
MluNI 2802 TGGCCA TGG^CCA
3410 TGGCCA
MscI 2802 TGGCCA TGG^CCA
3410 TGGCCA
MslI 2935 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3267 CACCATGATG
MvnI 1713 CGCG CG^CG
3208 CGCG
NciI 687 CCCGG CC^[GC]GG
688 CCGGG
NcoI 371 CCATGG C^CATGG
1046 CCATGG
NsiI 2856 ATGCAT ATGCA^T
3349 ATGCAT
PflFI 578 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1622 GACAAGGTC
SacI 678 GAGCTC GAGCT^C
1730 GAGCTC
SmlI 77 CTCAAG C^T[CT][GA]AG
2746 CTTGAG
SphI 1640 GCATGC GCATG^C
2156 GCATGC
SspBI 2868 TGTACA T^GTACA
3036 TGTACA
Tsp45I 2042 GTGAC ^GT[GC]AC
2063 GTGAC
Tth111I 578 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1622 GACAAGGTC
XmnI 2422 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2677 GAACGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BamHI
BanI BanII BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspDI BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I ClaI
DdeI DpnII DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII