Restriction map for
pF1KA0178
##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AccIII AclI AflII
Alw44I AocI ApaLI AscI AspI
AvrII BamHI BbrPI BcgI BciVI
BfrI BlnI BsaAI BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eco81I
EcoO65I EcoRI EcoT22I FseI HpaI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SbfI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 623 GGTACC G^GTACC
AcyI 2698 GGCGCC G[GA]^CG[CT]C
AflIII 2507 ACATGT A^C[GA][CT]GT
AhdI 3376 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3294 GGGCCC GGGCC^C
AseI 2242 ATTAAT AT^TAAT
AsnI 2242 ATTAAT AT^TAAT
Asp700I 3357 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 623 GGTACC G^GTACC
AspEI 3376 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 200 GAGCTC G[AT]GC[AT]^C
AvaI 2692 CTCGGG C^[CT]CG[GA]G
AviII 2357 TGCGCA TGC^GCA
BbeI 2698 GGCGCC GGCGC^C
BclI 3632 TGATCA T^GATCA
BglII 3248 AGATCT A^GATCT
BlpI 2602 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2602 GCTCAGC GC^T[ACGT]AGC
BsaHI 2698 GGCGCC G[GA]^CG[CT]C
BsgI 2101 GTGCAG GTGCAG(16/14)
BsiHKAI 200 GAGCTC G[AT]GC[AT]^C
BsoBI 2692 CTCGGG C^[CT]CG[GA]G
BspHI 2664 TCATGA T^CATGA
BspLU11I 2507 ACATGT A^CATGT
BspMI 164 ACCTGC ACCTGC(4/8)
BssSI 2921 CACGAG CACGAG(-5/-1)
BstBI 3175 TTCGAA TT^CGAA
CelII 2602 GCTCAGC GC^T[ACGT]AGC
EagI 1548 CGGCCG C^GGCCG
Eam1105I 3376 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1548 CGGCCG C^GGCCG
Eco52I 1548 CGGCCG C^GGCCG
EcoRV 2248 GATATC GAT^ATC
FbaI 3632 TGATCA T^GATCA
FspI 2357 TGCGCA TGC^GCA
HgaI 2094 GCGTC GACGC(5/10)
Hin1I 2698 GGCGCC G[GA]^CG[CT]C
KasI 2698 GGCGCC G^GCGCC
KpnI 623 GGTACC GGTAC^C
MslI 1632 CATTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2698 GGCGCC GG^CGCC
NheI 2220 GCTAGC G^CTAGC
NspI 2507 ACATGT [GA]CATG^[CT]
NspV 3175 TTCGAA TT^CGAA
PpuMI 185 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2242 ATTAAT AT^TAAT
RcaI 2664 TCATGA T^CATGA
SacI 200 GAGCTC GAGCT^C
ScaI 2138 AGTACT AGT^ACT
SfoI 2698 GGCGCC GGC^GCC
SfuI 3175 TTCGAA TT^CGAA
SspI 571 AATATT AAT^ATT
StuI 2792 AGGCCT AGG^CCT
XmnI 3357 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1183 AAATTC [GA]^AATT[CT]
2903 GAATTT
AgeI 620 ACCGGT A^CCGGT
1127 ACCGGT
Aor51HI 2036 AGCGCT AGC^GCT
3194 AGCGCT
ApoI 1183 AAATTC [GA]^AATT[CT]
2903 GAATTT
BalI 1394 TGGCCA TGG^CCA
2633 TGGCCA
BbvCI 265 GCTGAGG CCTCAGC(-5/-2)
2983 GCTGAGG
BglI 223 GCCAACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3163 GCCAAGCAGGC
BsaWI 620 ACCGGT [AT]^CCGG[AT]
1127 ACCGGT
BsiEI 1467 CGACCG CG[GA][CT]^CG
1548 CGGCCG
BsrDI 1881 CATTGC GCAATG(2/0)
1841 GCAATG
BsrFI 620 ACCGGT [GA]^CCGG[CT]
1127 ACCGGT
BstAPI 1266 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3039 GCAGAGTGTGC
BstXI 1604 CCAAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1631 CCATTATTGTGG
Cfr10I 620 ACCGGT [GA]^CCGG[CT]
1127 ACCGGT
Eco47III 2036 AGCGCT AGC^GCT
3194 AGCGCT
EcoNI 1164 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3613 CCTGAGCAAGG
HincII 50 GTCGAC GT[CT]^[GA]AC
3531 GTCGAC
HindII 50 GTCGAC GT[CT]^[GA]AC
3531 GTCGAC
HindIII 679 AAGCTT A^AGCTT
2752 AAGCTT
MaeII 1096 ACGT A^CGT
1862 ACGT
MluNI 1394 TGGCCA TGG^CCA
2633 TGGCCA
MscI 1394 TGGCCA TGG^CCA
2633 TGGCCA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
664 CAGCTG
PflMI 1170 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1434 CCAGGTGATGG
PinAI 620 ACCGGT A^CCGGT
1127 ACCGGT
PvuII 218 CAGCTG CAG^CTG
664 CAGCTG
SalI 50 GTCGAC G^TCGAC
3531 GTCGAC
SapI 1348 GAAGAGC GCTCTTC(1/4)
3310 GAAGAGC
SexAI 1433 ACCAGGT A^CC[AT]GGT
2309 ACCAGGT
SmlI 2134 CTCAAG C^T[CT][GA]AG
3565 CTCAAG
Van91I 1170 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1434 CCAGGTGATGG
XcmI 2411 CCAAGAAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3080 CCATGGAAAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII