Restriction map for
pF1KA0229
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# NO CUT ENZYMES ( 78 enzymes) #
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Acc65I AccI AccII AclI AflII
AflIII AhdI Alw44I ApaI ApaLI
AscI Asp700I Asp718I AspEI BbrPI
BciVI BfrI BsaAI BsaBI BsmI
Bsp1407I BspLU11I BsrGI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SmlI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 3017 TCCGGA T^CCGGA
AcsI 541 AAATTC [GA]^AATT[CT]
AgeI 3264 ACCGGT A^CCGGT
AocI 86 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2693 AGCGCT AGC^GCT
ApoI 541 AAATTC [GA]^AATT[CT]
AseI 900 ATTAAT AT^TAAT
AsnI 900 ATTAAT AT^TAAT
AviII 530 TGCGCA TGC^GCA
BamHI 2702 GGATCC G^GATCC
BcgI 2120 CGATTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2852 TGATCA T^GATCA
BlpI 192 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 192 GCTCAGC GC^T[ACGT]AGC
BseAI 3017 TCCGGA T^CCGGA
BsiEI 2605 CGGTCG CG[GA][CT]^CG
BsiWI 2498 CGTACG C^GTACG
BsmBI 1636 GAGACG CGTCTC(1/5)
BspDI 2977 ATCGAT AT^CGAT
BspEI 3017 TCCGGA T^CCGGA
BspHI 2021 TCATGA T^CATGA
BsrDI 3000 CATTGC GCAATG(2/0)
BssSI 3010 CACGAG CACGAG(-5/-1)
BstBI 3172 TTCGAA TT^CGAA
Bsu36I 86 CCTCAGG CC^T[ACGT]AGG
CelII 192 GCTCAGC GC^T[ACGT]AGC
ClaI 2977 ATCGAT AT^CGAT
DrdI 1474 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2693 AGCGCT AGC^GCT
Eco81I 86 CCTCAGG CC^T[ACGT]AGG
EcoNI 2274 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2852 TGATCA T^GATCA
FspI 530 TGCGCA TGC^GCA
HpaI 1374 GTTAAC GTT^AAC
MroI 3017 TCCGGA T^CCGGA
NspV 3172 TTCGAA TT^CGAA
PinAI 3264 ACCGGT A^CCGGT
PshBI 900 ATTAAT AT^TAAT
RcaI 2021 TCATGA T^CATGA
SacI 2425 GAGCTC GAGCT^C
SbfI 3195 CCTGCAGG CCTGCA^GG
SfiI 3192 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3172 TTCGAA TT^CGAA
SgrAI 2242 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1340 CCCGGG CCC^GGG
Sse8387I 3195 CCTGCAGG CCTGCA^GG
XmaCI 1340 CCCGGG C^CCGGG
XmaI 1340 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 814 GACGTC GACGT^C
2565 GACGTC
AspI 2167 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2356 GACTACGTC
AvrII 1207 CCTAGG C^CTAGG
1318 CCTAGG
BanII 2425 GAGCTC G[GA]GC[CT]^C
2841 GGGCTC
BbsI 1258 GAAGAC GAAGAC(2/6)
1348 GAAGAC
BglI 2325 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3193 GCCCTGCAGGC
BlnI 1207 CCTAGG C^CTAGG
1318 CCTAGG
BpuAI 1258 GAAGAC GAAGAC(2/6)
1348 GAAGAC
BsrBI 1731 CCGCTC CCGCTC(-3/-3)
2687 GAGCGG
BsrFI 2243 ACCGGC [GA]^CCGG[CT]
3264 ACCGGT
BstXI 1670 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2831 CCAATTATCTGG
Cfr10I 2243 ACCGGC [GA]^CCGG[CT]
3264 ACCGGT
NcoI 834 CCATGG C^CATGG
1919 CCATGG
PflFI 2167 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2356 GACTACGTC
PflMI 1110 CCATTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2175 CCACTTCCTGG
SapI 2200 GAAGAGC GCTCTTC(1/4)
164 GCTCTTC
SphI 1622 GCATGC GCATG^C
1647 GCATGC
Tth111I 2167 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2356 GACTACGTC
Van91I 1110 CCATTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2175 CCACTTCCTGG
XcmI 1619 CCAGCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2770 CCAGTGCAGAGTTGG
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
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AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BbeI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII