Restriction map for
pF1KA0256
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BbrPI BcgI BclI BcnI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI Cfr10I ClaI CpoI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FauI FbaI
FseI FspI HapII HincII HindII
HpaI HpaII KspI MamI MluI
MluNI MroI MscI MspI MvnI
NaeI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 2382 GGTACC G^GTACC
AccI 315 GTATAC GT^[AC][GT]AC
AcyI 3027 GGCGCC G[GA]^CG[CT]C
AflIII 1346 ACATGT A^C[GA][CT]GT
AocI 3031 CCTGAGG CC^T[ACGT]AGG
Asp718I 2382 GGTACC G^GTACC
AspHI 3133 GAGCAC G[AT]GC[AT]^C
BamHI 1657 GGATCC G^GATCC
BanII 8 GAGCCC G[GA]GC[CT]^C
BbeI 3027 GGCGCC GGCGC^C
BciVI 2554 GTATCC GTATCC(6/5)
BglII 2772 AGATCT A^GATCT
BlpI 292 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC
BsaHI 3027 GGCGCC G[GA]^CG[CT]C
BsiHKAI 3133 GAGCAC G[AT]GC[AT]^C
BsmBI 2010 GAGACG CGTCTC(1/5)
BsmI 436 GAATGC GAATGC(1/-1)
BspHI 2874 TCATGA T^CATGA
BspLU11I 1346 ACATGT A^CATGT
Bst1107I 315 GTATAC GTA^TAC
BstZ17I 315 GTATAC GTA^TAC
Bsu36I 3031 CCTGAGG CC^T[ACGT]AGG
CelII 292 GCTCAGC GC^T[ACGT]AGC
DraI 1602 TTTAAA TTT^AAA
DraII 609 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 341 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1797 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3031 CCTGAGG CC^T[ACGT]AGG
EcoNI 850 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 609 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2781 GAATTC G^AATTC
EcoRV 676 GATATC GAT^ATC
Hin1I 3027 GGCGCC G[GA]^CG[CT]C
KasI 3027 GGCGCC G^GCGCC
KpnI 2382 GGTACC GGTAC^C
MaeII 1191 ACGT A^CGT
MfeI 2612 CAATTG C^AATTG
MslI 905 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2612 CAATTG C^AATTG
NarI 3027 GGCGCC GG^CGCC
NcoI 2147 CCATGG C^CATGG
NdeI 2047 CATATG CA^TATG
PflMI 351 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 609 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1409 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2874 TCATGA T^CATGA
SapI 743 GAAGAGC GCTCTTC(1/4)
ScaI 2647 AGTACT AGT^ACT
SfoI 3027 GGCGCC GGC^GCC
SmlI 1241 CTCAAG C^T[CT][GA]AG
SspI 1087 AATATT AAT^ATT
Van91I 351 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 994 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 298 CCGC CCGC(-3/-1)
565 CCGC
AcsI 2165 AAATTC [GA]^AATT[CT]
2781 GAATTC
ApoI 2165 AAATTC [GA]^AATT[CT]
2781 GAATTC
BanI 2382 GGTACC G^G[CT][GA]CC
3027 GGCGCC
BbsI 2989 GAAGAC GAAGAC(2/6)
469 GTCTTC
BpmI 1766 CTCCAG CTGGAG(16/14)
3040 CTGGAG
BpuAI 2989 GAAGAC GAAGAC(2/6)
469 GTCTTC
BspMI 607 GCAGGT ACCTGC(4/8)
916 GCAGGT
CfoI 1629 GCGC GCG^C
3028 GCGC
DsaI 14 CCACGG C^C[GA][CT]GG
2147 CCATGG
HaeII 1628 GGCGCT [GA]GCGC^[CT]
3027 GGCGCC
HgaI 2204 GACGC GACGC(5/10)
447 GCGTC
HhaI 1629 GCGC GCG^C
3028 GCGC
HinP1I 1629 GCGC G^CGC
3028 GCGC
HindIII 1214 AAGCTT A^AGCTT
1286 AAGCTT
SphI 1742 GCATGC GCATG^C
2822 GCATGC
TaqI 489 TCGA T^CGA
621 TCGA
Tsp45I 1806 GTCAC ^GT[GC]AC
1933 GTGAC
TthHB8I 489 TCGA T^CGA
621 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AfaI AluI AlwI AlwNI Asp700I
AvaII BbvCI BbvI BfaI BmyI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I TspRI XcmI
XhoII XmnI