Restriction map for
pF1KA0322
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
AviII AvrII BamHI BbeI BfrI
BlnI BsaWI BseAI BsiWI BsmI
Bsp1407I BspEI BspHI BsrGI BssHII
Bst1107I BstZ17I CpoI DraIII EagI
EclXI Eco52I EcoRV EcoT22I FseI
FspI KasI MfeI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmeI PshAI PshBI Psp1406I RcaI
RsrII SalI SfiI SfoI SnaBI
SpeI SspBI SwaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 2244 GGTACC G^GTACC
AccI 4399 GTAGAC GT^[AC][GT]AC
AhdI 2275 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 503 GGGCCC GGGCC^C
Asp700I 367 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2244 GGTACC G^GTACC
AspEI 2275 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1246 GCTGAGG CCTCAGC(-5/-2)
BciVI 4746 GTATCC GTATCC(6/5)
BclI 611 TGATCA T^GATCA
BglI 58 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspLU11I 3032 ACATGT A^CATGT
BsrFI 98 GCCGGT [GA]^CCGG[CT]
BstBI 3305 TTCGAA TT^CGAA
Cfr10I 98 GCCGGT [GA]^CCGG[CT]
DraI 559 TTTAAA TTT^AAA
DrdI 593 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2275 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4156 GAATTC G^AATTC
FbaI 611 TGATCA T^GATCA
HincII 4779 GTTAAC GT[CT]^[GA]AC
HindII 4779 GTTAAC GT[CT]^[GA]AC
HindIII 3411 AAGCTT A^AGCTT
HpaI 4779 GTTAAC GTT^AAC
KpnI 2244 GGTACC GGTAC^C
MluI 2021 ACGCGT A^CGCGT
NspV 3305 TTCGAA TT^CGAA
PaeR7I 4404 CTCGAG C^TCGAG
PvuI 1206 CGATCG CGAT^CG
SbfI 3555 CCTGCAGG CCTGCA^GG
ScaI 3947 AGTACT AGT^ACT
SfuI 3305 TTCGAA TT^CGAA
SgrAI 97 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 3062 CCCGGG CCC^GGG
Sse8387I 3555 CCTGCAGG CCTGCA^GG
SspI 4142 AATATT AAT^ATT
XhoI 4404 CTCGAG C^TCGAG
XmaCI 3062 CCCGGG C^CCGGG
XmaI 3062 CCCGGG C^CCGGG
XmnI 367 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AflIII 2021 ACGCGT A^C[GA][CT]GT
3032 ACATGT
AocI 156 CCTCAGG CC^T[ACGT]AGG
2448 CCTGAGG
AspI 211 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
283 GACCTGGTC
BbrPI 2335 CACGTG CAC^GTG
2548 CACGTG
BcgI 94 CGACGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2129 GCAACAGGTTCG
BlpI 1797 GCTCAGC GC^T[ACGT]AGC
3413 GCTTAGC
Bpu1102I 1797 GCTCAGC GC^T[ACGT]AGC
3413 GCTTAGC
BsaAI 2335 CACGTG [CT]AC^GT[GA]
2548 CACGTG
BsaBI 3496 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4519 GATGGGCATC
BsmBI 68 CGTCTC CGTCTC(1/5)
1627 GAGACG
BspDI 433 ATCGAT AT^CGAT
2479 ATCGAT
BspMI 2941 ACCTGC ACCTGC(4/8)
2374 GCAGGT
BstEII 216 GGTCACC G^GT[ACGT]ACC
4510 GGTTACC
BstPI 216 GGTCACC G^GT[ACGT]ACC
4510 GGTTACC
Bsu36I 156 CCTCAGG CC^T[ACGT]AGG
2448 CCTGAGG
CelII 1797 GCTCAGC GC^T[ACGT]AGC
3413 GCTTAGC
ClaI 433 ATCGAT AT^CGAT
2479 ATCGAT
Eco81I 156 CCTCAGG CC^T[ACGT]AGG
2448 CCTGAGG
EcoO65I 216 GGTCACC G^GT[ACGT]ACC
4510 GGTTACC
KspI 1666 CCGCGG CCGC^GG
4460 CCGCGG
MamI 3496 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4519 GATGGGCATC
PflFI 211 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
283 GACCTGGTC
PmaCI 2335 CACGTG CAC^GTG
2548 CACGTG
PmlI 2335 CACGTG CAC^GTG
2548 CACGTG
SacII 1666 CCGCGG CCGC^GG
4460 CCGCGG
SexAI 212 ACCTGGT A^CC[AT]GGT
284 ACCTGGT
SphI 1706 GCATGC GCATG^C
2603 GCATGC
Tth111I 211 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
283 GACCTGGTC
XbaI 76 TCTAGA T^CTAGA
3247 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII