Restriction map for
pF1KA0333
##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
ApaI AscI AseI AsnI AspI
AviII AvrII BbvCI BcgI BclI
BfrI BlnI BsiWI BsmBI BsmI
BspDI BspLU11I BssHII Bst1107I BstXI
BstZ17I ClaI DraI DrdI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
SalI SbfI ScaI SfiI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 2491 GACGTC GACGT^C
Acc65I 1926 GGTACC G^GTACC
AccIII 2685 TCCGGA T^CCGGA
AhdI 1600 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1396 GTGCAC G^TGCAC
Aor51HI 349 AGCGCT AGC^GCT
ApaLI 1396 GTGCAC G^TGCAC
Asp700I 868 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1926 GGTACC G^GTACC
AspEI 1600 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2805 TGGCCA TGG^CCA
BamHI 548 GGATCC G^GATCC
BsaBI 2803 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2685 TCCGGA [AT]^CCGG[AT]
BseAI 2685 TCCGGA T^CCGGA
Bsp1407I 1048 TGTACA T^GTACA
BspEI 2685 TCCGGA T^CCGGA
BspHI 2078 TCATGA T^CATGA
BsrGI 1048 TGTACA T^GTACA
BssSI 1696 CTCGTG CACGAG(-5/-1)
BstBI 86 TTCGAA TT^CGAA
BstEII 2871 GGTCACC G^GT[ACGT]ACC
BstPI 2871 GGTCACC G^GT[ACGT]ACC
CpoI 1842 CGGACCG CG^G[AT]CCG
DraIII 2968 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2892 CGGCCG C^GGCCG
Eam1105I 1600 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2892 CGGCCG C^GGCCG
Eco47III 349 AGCGCT AGC^GCT
Eco52I 2892 CGGCCG C^GGCCG
EcoNI 3246 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2871 GGTCACC G^GT[ACGT]ACC
KpnI 1926 GGTACC GGTAC^C
MamI 2803 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2805 TGGCCA TGG^CCA
MroI 2685 TCCGGA T^CCGGA
MscI 2805 TGGCCA TGG^CCA
NaeI 3548 GCCGGC GCC^GGC
NgoMIV 3548 GCCGGC G^CCGGC
NspV 86 TTCGAA TT^CGAA
PaeR7I 905 CTCGAG C^TCGAG
RcaI 2078 TCATGA T^CATGA
RsrII 1842 CGGACCG CG^G[AT]CCG
SacI 3346 GAGCTC GAGCT^C
SapI 2469 GAAGAGC GCTCTTC(1/4)
SexAI 3537 ACCAGGT A^CC[AT]GGT
SfuI 86 TTCGAA TT^CGAA
SmaI 1198 CCCGGG CCC^GGG
SphI 263 GCATGC GCATG^C
SspBI 1048 TGTACA T^GTACA
StuI 2742 AGGCCT AGG^CCT
XhoI 905 CTCGAG C^TCGAG
XmaCI 1198 CCCGGG C^CCGGG
XmaI 1198 CCCGGG C^CCGGG
XmnI 868 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 37 CCTAAGG CC^T[ACGT]AGG
1760 CCTCAGG
BbeI 245 GGCGCC GGCGC^C
1170 GGCGCC
BciVI 1250 GTATCC GTATCC(6/5)
3219 GTATCC
BglI 1831 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1840 GCCGGACCGGC
BglII 1148 AGATCT A^GATCT
2326 AGATCT
BlpI 3040 GCTAAGC GC^T[ACGT]AGC
3489 GCTGAGC
Bpu1102I 3040 GCTAAGC GC^T[ACGT]AGC
3489 GCTGAGC
BsiEI 766 CGACCG CG[GA][CT]^CG
2892 CGGCCG
BspMI 1851 ACCTGC ACCTGC(4/8)
1375 GCAGGT
BsrBI 2909 CCGCTC CCGCTC(-3/-3)
2862 GAGCGG
BsrDI 237 CATTGC GCAATG(2/0)
23 GCAATG
BsrFI 1845 ACCGGC [GA]^CCGG[CT]
3548 GCCGGC
BstAPI 1861 GCAACGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2918 GCAGTTCATGC
Bsu36I 37 CCTAAGG CC^T[ACGT]AGG
1760 CCTCAGG
CelII 3040 GCTAAGC GC^T[ACGT]AGC
3489 GCTGAGC
Cfr10I 1845 ACCGGC [GA]^CCGG[CT]
3548 GCCGGC
Eco81I 37 CCTAAGG CC^T[ACGT]AGG
1760 CCTCAGG
KasI 245 GGCGCC G^GCGCC
1170 GGCGCC
KspI 249 CCGCGG CCGC^GG
1664 CCGCGG
NarI 245 GGCGCC GG^CGCC
1170 GGCGCC
NcoI 1628 CCATGG C^CATGG
1792 CCATGG
PstI 1236 CTGCAG CTGCA^G
1853 CTGCAG
SacII 249 CCGCGG CCGC^GG
1664 CCGCGG
SfcI 1236 CTGCAG C^T[GA][CT]AG
1853 CTGCAG
SfoI 245 GGCGCC GGC^GCC
1170 GGCGCC
XcmI 614 CCAGTGACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2393 CCATCCCCGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII