Restriction map for
pF1KA0338
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglI BlnI
BsaAI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstXI Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HpaI KpnI Ksp632I KspI MluI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SfiI
SgrAI SnaBI SpeI SspBI SspI
StuI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 161 CGCG CG^CG
AccIII 485 TCCGGA T^CCGGA
AcsI 501 GAATTT [GA]^AATT[CT]
AcyI 2389 GGCGCC G[GA]^CG[CT]C
AgeI 1070 ACCGGT A^CCGGT
ApoI 501 GAATTT [GA]^AATT[CT]
AspI 370 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2389 GGCGCC GGCGC^C
BglII 977 AGATCT A^GATCT
BlpI 664 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 664 GCTGAGC GC^T[ACGT]AGC
BsaBI 2503 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2389 GGCGCC G[GA]^CG[CT]C
BsaI 2248 GAGACC GGTCTC(1/5)
BseAI 485 TCCGGA T^CCGGA
BsiEI 1929 CGACCG CG[GA][CT]^CG
BsmI 825 GAATGC GAATGC(1/-1)
BspEI 485 TCCGGA T^CCGGA
Bst1107I 2587 GTATAC GTA^TAC
BstBI 1897 TTCGAA TT^CGAA
BstEII 2571 GGTAACC G^GT[ACGT]ACC
BstPI 2571 GGTAACC G^GT[ACGT]ACC
BstUI 161 CGCG CG^CG
BstZ17I 2587 GTATAC GTA^TAC
CelII 664 GCTGAGC GC^T[ACGT]AGC
DrdI 1861 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2571 GGTAACC G^GT[ACGT]ACC
Hin1I 2389 GGCGCC G[GA]^CG[CT]C
HindIII 835 AAGCTT A^AGCTT
KasI 2389 GGCGCC G^GCGCC
MamI 2503 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1046 CAATTG C^AATTG
MroI 485 TCCGGA T^CCGGA
MseI 1055 TTAA T^TAA
MslI 87 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1046 CAATTG C^AATTG
MvnI 161 CGCG CG^CG
NaeI 608 GCCGGC GCC^GGC
NarI 2389 GGCGCC GG^CGCC
NgoMIV 608 GCCGGC G^CCGGC
NspV 1897 TTCGAA TT^CGAA
PflFI 370 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2517 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1070 ACCGGT A^CCGGT
PshAI 2154 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 100 CCTGCAGG CCTGCA^GG
SexAI 371 ACCTGGT A^CC[AT]GGT
SfoI 2389 GGCGCC GGC^GCC
SfuI 1897 TTCGAA TT^CGAA
SphI 633 GCATGC GCATG^C
Sse8387I 100 CCTGCAGG CCTGCA^GG
Tru9I 1055 TTAA T^TAA
Tth111I 370 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2517 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 853 GTAGAC GT^[AC][GT]AC
2587 GTATAC
ApaI 1721 GGGCCC GGGCC^C
1991 GGGCCC
BanI 1645 GGCACC G^G[CT][GA]CC
2389 GGCGCC
BbsI 553 GAAGAC GAAGAC(2/6)
1900 GAAGAC
BpuAI 553 GAAGAC GAAGAC(2/6)
1900 GAAGAC
BspMI 857 ACCTGC ACCTGC(4/8)
2184 GCAGGT
EcoNI 396 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
983 CCTACAAGAGG
MaeII 1713 ACGT A^CGT
1837 ACGT
NcoI 1884 CCATGG C^CATGG
2264 CCATGG
NspI 633 GCATGC [GA]CATG^[CT]
693 GCATGT
PpuMI 1192 AGGACCC [GA]G^G[AT]CC[CT]
1546 AGGACCC
SmlI 973 CTCAAG C^T[CT][GA]AG
2278 CTCAAG
XcmI 1148 CCAAGGGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2181 CCAGCAGGTTGATGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbvCI BbvI BcgI BcnI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI