Restriction map for
pF1KA0343
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbvCI BcgI BfrI BglI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstZ17I Bsu36I CpoI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoRV FseI FspI KpnI
KspI MamI MluI MroI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2630 GACGTC GACGT^C
ApaI 2751 GGGCCC GGGCC^C
BamHI 2859 GGATCC G^GATCC
BbeI 2300 GGCGCC GGCGC^C
BbrPI 1785 CACGTG CAC^GTG
BciVI 2350 GTATCC GTATCC(6/5)
BcnI 2689 CCGGG CC^[GC]GG
BseRI 1385 CTCCTC GAGGAG(10/8)
BsgI 1223 GTGCAG GTGCAG(16/14)
BsmBI 1815 CGTCTC CGTCTC(1/5)
Bsp1407I 367 TGTACA T^GTACA
BspDI 265 ATCGAT AT^CGAT
BsrGI 367 TGTACA T^GTACA
BstAPI 967 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2260 TTCGAA TT^CGAA
BstEII 282 GGTCACC G^GT[ACGT]ACC
BstPI 282 GGTCACC G^GT[ACGT]ACC
ClaI 265 ATCGAT AT^CGAT
DraIII 566 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 274 GACCCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 282 GGTCACC G^GT[ACGT]ACC
EcoRI 3528 GAATTC G^AATTC
EcoT14I 2661 CCAAGG C^C[AT][AT]GG
EcoT22I 974 ATGCAT ATGCA^T
HindIII 228 AAGCTT A^AGCTT
HpaI 1125 GTTAAC GTT^AAC
KasI 2300 GGCGCC G^GCGCC
MfeI 509 CAATTG C^AATTG
MunI 509 CAATTG C^AATTG
NarI 2300 GGCGCC GG^CGCC
NciI 2689 CCGGG CC^[GC]GG
NsiI 974 ATGCAT ATGCA^T
NspI 2681 GCATGT [GA]CATG^[CT]
NspV 2260 TTCGAA TT^CGAA
PflMI 1397 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1785 CACGTG CAC^GTG
PmlI 1785 CACGTG CAC^GTG
ScaI 489 AGTACT AGT^ACT
SexAI 1334 ACCAGGT A^CC[AT]GGT
SfoI 2300 GGCGCC GGC^GCC
SfuI 2260 TTCGAA TT^CGAA
SmlI 584 CTCAAG C^T[CT][GA]AG
SspBI 367 TGTACA T^GTACA
StyI 2661 CCAAGG C^C[AT][AT]GG
SwaI 2993 ATTTAAAT ATTT^AAAT
Van91I 1397 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 83 GTGCAC G^TGCAC
2533 GTGCAC
Aor51HI 29 AGCGCT AGC^GCT
1825 AGCGCT
ApaLI 83 GTGCAC G^TGCAC
2533 GTGCAC
Asp700I 2253 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3344 GAACTCCTTC
AspI 1810 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACAGAGTC
AvaI 179 CTCGGG C^[CT]CG[GA]G
2434 CCCGAG
BalI 1793 TGGCCA TGG^CCA
3417 TGGCCA
BanII 2698 GAGCCC G[GA]GC[CT]^C
2751 GGGCCC
BclI 1656 TGATCA T^GATCA
2396 TGATCA
BlpI 1294 GCTGAGC GC^T[ACGT]AGC
1833 GCTTAGC
Bpu1102I 1294 GCTGAGC GC^T[ACGT]AGC
1833 GCTTAGC
BsaI 343 GAGACC GGTCTC(1/5)
1382 GGTCTC
BsmI 869 GAATGC GAATGC(1/-1)
1639 GAATGC
BsoBI 179 CTCGGG C^[CT]CG[GA]G
2434 CCCGAG
BspHI 323 TCATGA T^CATGA
1434 TCATGA
BspMI 1317 ACCTGC ACCTGC(4/8)
3108 GCAGGT
BsrFI 3104 GCCGGC [GA]^CCGG[CT]
3477 GCCGGC
BstYI 2648 AGATCC [GA]^GATC[CT]
2859 GGATCC
CelII 1294 GCTGAGC GC^T[ACGT]AGC
1833 GCTTAGC
Cfr10I 3104 GCCGGC [GA]^CCGG[CT]
3477 GCCGGC
EaeI 1793 TGGCCA [CT]^GGCC[GA]
3417 TGGCCA
Eco47III 29 AGCGCT AGC^GCT
1825 AGCGCT
EcoNI 295 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
621 CCTCCCAGAGG
FbaI 1656 TGATCA T^GATCA
2396 TGATCA
HgaI 1814 GCGTC GACGC(5/10)
2146 GCGTC
MflI 2648 AGATCC [GA]^GATC[CT]
2859 GGATCC
MluNI 1793 TGGCCA TGG^CCA
3417 TGGCCA
MscI 1793 TGGCCA TGG^CCA
3417 TGGCCA
NaeI 3104 GCCGGC GCC^GGC
3477 GCCGGC
NgoMIV 3104 GCCGGC G^CCGGC
3477 GCCGGC
PflFI 1810 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACAGAGTC
RcaI 323 TCATGA T^CATGA
1434 TCATGA
SapI 2127 GAAGAGC GCTCTTC(1/4)
1429 GCTCTTC
SspI 187 AATATT AAT^ATT
531 AATATT
StuI 1351 AGGCCT AGG^CCT
2277 AGGCCT
Tth111I 1810 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACAGAGTC
XhoII 2648 AGATCC [GA]^GATC[CT]
2859 GGATCC
XmnI 2253 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3344 GAACTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI