Restriction map for
pF1KA0350
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BciVI BfrI BglII
BlnI BseAI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MluI MroI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SpeI Sse8387I SspI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AclI 1183 AACGTT AA^CGTT
Alw44I 1750 GTGCAC G^TGCAC
Aor51HI 2606 AGCGCT AGC^GCT
ApaI 1213 GGGCCC GGGCC^C
ApaLI 1750 GTGCAC G^TGCAC
BamHI 1542 GGATCC G^GATCC
BbeI 2576 GGCGCC GGCGC^C
BbvCI 3151 GCTGAGG CCTCAGC(-5/-2)
BsaWI 128 ACCGGA [AT]^CCGG[AT]
BsmI 1082 GCATTC GAATGC(1/-1)
BspLU11I 1835 ACATGT A^CATGT
BsrDI 1425 GCAATG GCAATG(2/0)
BssSI 328 CACGAG CACGAG(-5/-1)
Eco47III 2606 AGCGCT AGC^GCT
EcoNI 2259 CCTATTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 2576 GGCGCC G^GCGCC
MfeI 2035 CAATTG C^AATTG
MunI 2035 CAATTG C^AATTG
NaeI 3087 GCCGGC GCC^GGC
NarI 2576 GGCGCC GG^CGCC
NcoI 36 CCATGG C^CATGG
NgoMIV 3087 GCCGGC G^CCGGC
NheI 1614 GCTAGC G^CTAGC
PaeR7I 1114 CTCGAG C^TCGAG
PpuMI 2081 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3021 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1183 AACGTT AA^CGTT
PstI 292 CTGCAG CTGCA^G
PvuII 286 CAGCTG CAG^CTG
SapI 858 GCTCTTC GCTCTTC(1/4)
SfcI 292 CTGCAG C^T[GA][CT]AG
SfoI 2576 GGCGCC GGC^GCC
SnaBI 364 TACGTA TAC^GTA
SphI 3068 GCATGC GCATG^C
StuI 2256 AGGCCT AGG^CCT
XhoI 1114 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccII 14 CGCG CG^CG
1461 CGCG
AcsI 369 AAATTC [GA]^AATT[CT]
388 AAATTT
ApoI 369 AAATTC [GA]^AATT[CT]
388 AAATTT
Asp700I 1003 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1783 GAAAGTGTTC
AvaI 1114 CTCGAG C^[CT]CG[GA]G
2072 CTCGGG
BalI 2423 TGGCCA TGG^CCA
2903 TGGCCA
BsaBI 168 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1311 GATGGTGATC
BsiEI 913 CGGCCG CG[GA][CT]^CG
1586 CGGCCG
BsoBI 1114 CTCGAG C^[CT]CG[GA]G
2072 CTCGGG
Bsp1407I 512 TGTACA T^GTACA
2137 TGTACA
BsrBI 9 CCGCTC CCGCTC(-3/-3)
1117 GAGCGG
BsrGI 512 TGTACA T^GTACA
2137 TGTACA
BstAPI 1082 GCATTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2265 GCAGGACATGC
BstUI 14 CGCG CG^CG
1461 CGCG
BstXI 1653 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1874 CCATGAACGTGG
DraII 1212 AGGGCCC [GA]G^G[ACGT]CC[CT]
2081 AGGACCT
EagI 913 CGGCCG C^GGCCG
1586 CGGCCG
EclXI 913 CGGCCG C^GGCCG
1586 CGGCCG
Eco52I 913 CGGCCG C^GGCCG
1586 CGGCCG
EcoO109I 1212 AGGGCCC [GA]G^G[ACGT]CC[CT]
2081 AGGACCT
HincII 2863 GTCAAC GT[CT]^[GA]AC
3037 GTTGAC
HindII 2863 GTCAAC GT[CT]^[GA]AC
3037 GTTGAC
MamI 168 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1311 GATGGTGATC
MluNI 2423 TGGCCA TGG^CCA
2903 TGGCCA
MscI 2423 TGGCCA TGG^CCA
2903 TGGCCA
MvnI 14 CGCG CG^CG
1461 CGCG
PflMI 60 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1458 CCACGCGCTGG
SspBI 512 TGTACA T^GTACA
2137 TGTACA
Van91I 60 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1458 CCACGCGCTGG
XmnI 1003 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1783 GAAAGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbsI BbvI BcgI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII