Restriction map for
pF1KA0365
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# NO CUT ENZYMES ( 92 enzymes) #
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AccI AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AviII BamHI
BbeI BbrPI BcgI BciVI BclI
BsaAI BsaBI BsaI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HgaI HindIII HpaI KasI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XcmI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 166 GACGTC GACGT^C
Acc65I 2833 GGTACC G^GTACC
AccII 2639 CGCG CG^CG
AcyI 166 GACGTC G[GA]^CG[CT]C
AflII 126 CTTAAG C^TTAAG
AflIII 1785 ACGTGT A^C[GA][CT]GT
Asp718I 2833 GGTACC G^GTACC
AspI 358 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 675 CCTAGG C^CTAGG
BbvCI 1955 CCTCAGC CCTCAGC(-5/-2)
BfrI 126 CTTAAG C^TTAAG
BglI 2768 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 120 AGATCT A^GATCT
BlnI 675 CCTAGG C^CTAGG
BlpI 2647 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2647 GCTGAGC GC^T[ACGT]AGC
BsaHI 166 GACGTC G[GA]^CG[CT]C
BsaWI 374 ACCGGA [AT]^CCGG[AT]
BsgI 1967 GTGCAG GTGCAG(16/14)
BsiEI 2139 CGGCCG CG[GA][CT]^CG
Bsp1407I 3221 TGTACA T^GTACA
BsrBI 2720 CCGCTC CCGCTC(-3/-3)
BsrDI 1340 GCAATG GCAATG(2/0)
BsrGI 3221 TGTACA T^GTACA
BstAPI 1960 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2639 CGCG CG^CG
CelII 2647 GCTGAGC GC^T[ACGT]AGC
CfoI 415 GCGC GCG^C
EagI 2139 CGGCCG C^GGCCG
EclXI 2139 CGGCCG C^GGCCG
Eco52I 2139 CGGCCG C^GGCCG
HhaI 415 GCGC GCG^C
Hin1I 166 GACGTC G[GA]^CG[CT]C
HinP1I 415 GCGC G^CGC
HincII 655 GTTGAC GT[CT]^[GA]AC
HindII 655 GTTGAC GT[CT]^[GA]AC
KpnI 2833 GGTACC GGTAC^C
KspI 2638 CCGCGG CCGC^GG
MvnI 2639 CGCG CG^CG
NaeI 2141 GCCGGC GCC^GGC
NcoI 3081 CCATGG C^CATGG
NgoMIV 2141 GCCGGC G^CCGGC
NspI 1211 ACATGC [GA]CATG^[CT]
PflFI 358 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3081 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 641 GAGCTC GAGCT^C
SacII 2638 CCGCGG CCGC^GG
SexAI 359 ACCAGGT A^CC[AT]GGT
SmaI 1991 CCCGGG CCC^GGG
SspBI 3221 TGTACA T^GTACA
SspI 1883 AATATT AAT^ATT
Tth111I 358 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3081 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 214 TCTAGA T^CTAGA
XmaCI 1991 CCCGGG C^CCGGG
XmaI 1991 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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Asp700I 97 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2935 GAACCAGTTC
BalI 369 TGGCCA TGG^CCA
444 TGGCCA
BstBI 1549 TTCGAA TT^CGAA
2942 TTCGAA
EcoRV 62 GATATC GAT^ATC
868 GATATC
MluNI 369 TGGCCA TGG^CCA
444 TGGCCA
MscI 369 TGGCCA TGG^CCA
444 TGGCCA
NspV 1549 TTCGAA TT^CGAA
2942 TTCGAA
SfuI 1549 TTCGAA TT^CGAA
2942 TTCGAA
XmnI 97 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2935 GAACCAGTTC
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII