Restriction map for
pF1KA0371
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccI AccII AccIII AcyI
AflII AhdI AscI AseI AsnI
AspEI AspI AviII BbeI BbrPI
BciVI BclI BfrI BsaAI BsaHI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraIII DrdI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI PacI PflFI PmaCI PmeI
PmlI PshAI PshBI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 2461 GGTACC G^GTACC
AclI 1412 AACGTT AA^CGTT
AcsI 1363 AAATTT [GA]^AATT[CT]
AgeI 1373 ACCGGT A^CCGGT
AocI 2194 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1182 AGCGCT AGC^GCT
ApaI 2575 GGGCCC GGGCC^C
ApoI 1363 AAATTT [GA]^AATT[CT]
Asp718I 2461 GGTACC G^GTACC
AvaI 833 CTCGAG C^[CT]CG[GA]G
AvrII 1995 CCTAGG C^CTAGG
BamHI 1974 GGATCC G^GATCC
BbsI 2187 GAAGAC GAAGAC(2/6)
BcgI 1761 CGACAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1995 CCTAGG C^CTAGG
BpuAI 2187 GAAGAC GAAGAC(2/6)
BsaWI 1373 ACCGGT [AT]^CCGG[AT]
BsoBI 833 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3065 TGTACA T^GTACA
BsrGI 3065 TGTACA T^GTACA
BssSI 1330 CTCGTG CACGAG(-5/-1)
BstBI 2387 TTCGAA TT^CGAA
Bsu36I 2194 CCTGAGG CC^T[ACGT]AGG
DraI 481 TTTAAA TTT^AAA
DsaI 2636 CCATGG C^C[GA][CT]GG
EagI 1219 CGGCCG C^GGCCG
EclXI 1219 CGGCCG C^GGCCG
Eco47III 1182 AGCGCT AGC^GCT
Eco52I 1219 CGGCCG C^GGCCG
Eco81I 2194 CCTGAGG CC^T[ACGT]AGG
HgaI 3093 GCGTC GACGC(5/10)
HincII 816 GTCAAC GT[CT]^[GA]AC
HindII 816 GTCAAC GT[CT]^[GA]AC
KpnI 2461 GGTACC GGTAC^C
NcoI 2636 CCATGG C^CATGG
NspV 2387 TTCGAA TT^CGAA
PaeR7I 833 CTCGAG C^TCGAG
PflMI 1263 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1373 ACCGGT A^CCGGT
Psp1406I 1412 AACGTT AA^CGTT
PvuI 796 CGATCG CGAT^CG
SacI 580 GAGCTC GAGCT^C
SfuI 2387 TTCGAA TT^CGAA
SgrAI 1930 CACCGGCG C[GA]^CCGG[CT]G
SphI 1197 GCATGC GCATG^C
SspBI 3065 TGTACA T^GTACA
StuI 2198 AGGCCT AGG^CCT
Van91I 1263 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 712 CCAGAGGTTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 833 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1095 GTGCAC G^TGCAC
1769 GTGCAC
ApaLI 1095 GTGCAC G^TGCAC
1769 GTGCAC
Asp700I 2419 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3435 GAACGTATTC
BalI 1356 TGGCCA TGG^CCA
1880 TGGCCA
BbvCI 2201 CCTCAGC CCTCAGC(-5/-2)
2053 GCTGAGG
BglI 1955 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2119 GCCCACAGGGC
BlpI 1279 GCTAAGC GC^T[ACGT]AGC
1947 GCTGAGC
Bpu1102I 1279 GCTAAGC GC^T[ACGT]AGC
1947 GCTGAGC
BsiEI 796 CGATCG CG[GA][CT]^CG
1219 CGGCCG
BspLU11I 1597 ACATGT A^CATGT
2540 ACATGT
BstXI 675 CCAGAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1748 CCACCCCTGTGG
CelII 1279 GCTAAGC GC^T[ACGT]AGC
1947 GCTGAGC
FauI 698 CCCGC CCCGC(4/6)
3032 CCCGC
HindIII 776 AAGCTT A^AGCTT
931 AAGCTT
MluNI 1356 TGGCCA TGG^CCA
1880 TGGCCA
MscI 1356 TGGCCA TGG^CCA
1880 TGGCCA
NaeI 1817 GCCGGC GCC^GGC
1886 GCCGGC
NgoMIV 1817 GCCGGC G^CCGGC
1886 GCCGGC
XmnI 2419 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3435 GAACGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII