Restriction map for
pF1KA0384
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AlwNI Aor51HI AscI Asp718I BbrPI
BcgI BciVI BglII BlpI Bpu1102I
BsaAI BseAI BsgI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrGI BssHII
Bst1107I BstZ17I CelII CpoI DraI
DraIII DrdI EarI Eco47III EcoNI
EcoRI EcoRV EcoT22I FseI HpaI
KpnI Ksp632I KspI MfeI MroI
MunI NcoI NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI Psp1406I PstI RsrII SacII
SapI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI XbaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 127 CGCG CG^CG
AflII 2014 CTTAAG C^TTAAG
AflIII 126 ACGCGT A^C[GA][CT]GT
Alw44I 1270 GTGCAC G^TGCAC
ApaI 772 GGGCCC GGGCC^C
ApaLI 1270 GTGCAC G^TGCAC
AseI 2235 ATTAAT AT^TAAT
AsnI 2235 ATTAAT AT^TAAT
Asp700I 2656 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1058 TGCGCA TGC^GCA
AvrII 607 CCTAGG C^CTAGG
BalI 164 TGGCCA TGG^CCA
BbvCI 2048 CCTCAGC CCTCAGC(-5/-2)
BfrI 2014 CTTAAG C^TTAAG
BglI 834 GCCTTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 607 CCTAGG C^CTAGG
BsmBI 108 CGTCTC CGTCTC(1/5)
BspDI 2753 ATCGAT AT^CGAT
BspHI 953 TCATGA T^CATGA
BspMI 1166 ACCTGC ACCTGC(4/8)
BssSI 1388 CTCGTG CACGAG(-5/-1)
BstAPI 1477 GCACTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1379 TTCGAA TT^CGAA
BstEII 699 GGTGACC G^GT[ACGT]ACC
BstPI 699 GGTGACC G^GT[ACGT]ACC
BstUI 127 CGCG CG^CG
BstXI 29 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2753 ATCGAT AT^CGAT
DsaI 416 CCACGG C^C[GA][CT]GG
EagI 198 CGGCCG C^GGCCG
EclXI 198 CGGCCG C^GGCCG
Eco52I 198 CGGCCG C^GGCCG
EcoO65I 699 GGTGACC G^GT[ACGT]ACC
FspI 1058 TGCGCA TGC^GCA
HincII 21 GTCGAC GT[CT]^[GA]AC
HindII 21 GTCGAC GT[CT]^[GA]AC
MluI 126 ACGCGT A^CGCGT
MluNI 164 TGGCCA TGG^CCA
MscI 164 TGGCCA TGG^CCA
MslI 2587 CATTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 127 CGCG CG^CG
NaeI 185 GCCGGC GCC^GGC
NdeI 2591 CATATG CA^TATG
NgoMIV 185 GCCGGC G^CCGGC
NspV 1379 TTCGAA TT^CGAA
PaeR7I 1958 CTCGAG C^TCGAG
PflMI 525 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 564 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2235 ATTAAT AT^TAAT
PvuI 2755 CGATCG CGAT^CG
RcaI 953 TCATGA T^CATGA
SalI 21 GTCGAC G^TCGAC
SexAI 567 ACCTGGT A^CC[AT]GGT
SfuI 1379 TTCGAA TT^CGAA
SphI 1482 GCATGC GCATG^C
Van91I 525 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1958 CTCGAG C^TCGAG
XmnI 2656 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 21 GTCGAC GT^[AC][GT]AC
755 GTAGAC
AcyI 101 GGCGCC G[GA]^CG[CT]C
947 GGCGCC
AhdI 528 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2088 GACGTATGGTC
AocI 349 CCTGAGG CC^T[ACGT]AGG
1599 CCTTAGG
AspEI 528 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2088 GACGTATGGTC
AspI 566 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2317 GACACTGTC
BamHI 345 GGATCC G^GATCC
1911 GGATCC
BbeI 101 GGCGCC GGCGC^C
947 GGCGCC
BclI 1502 TGATCA T^GATCA
2420 TGATCA
BsaHI 101 GGCGCC G[GA]^CG[CT]C
947 GGCGCC
BsaWI 1417 ACCGGA [AT]^CCGG[AT]
2621 ACCGGA
BsrDI 557 GCAATG GCAATG(2/0)
1187 GCAATG
BsrFI 185 GCCGGC [GA]^CCGG[CT]
200 GCCGGT
Bsu36I 349 CCTGAGG CC^T[ACGT]AGG
1599 CCTTAGG
Cfr10I 185 GCCGGC [GA]^CCGG[CT]
200 GCCGGT
EaeI 164 TGGCCA [CT]^GGCC[GA]
198 CGGCCG
Eam1105I 528 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2088 GACGTATGGTC
Eco81I 349 CCTGAGG CC^T[ACGT]AGG
1599 CCTTAGG
EcoT14I 607 CCTAGG C^C[AT][AT]GG
1275 CCTTGG
FbaI 1502 TGATCA T^GATCA
2420 TGATCA
HgaI 128 GCGTC GACGC(5/10)
2118 GCGTC
Hin1I 101 GGCGCC G[GA]^CG[CT]C
947 GGCGCC
KasI 101 GGCGCC G^GCGCC
947 GGCGCC
MseI 2015 TTAA T^TAA
2236 TTAA
NarI 101 GGCGCC GG^CGCC
947 GGCGCC
PflFI 566 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2317 GACACTGTC
PshAI 414 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
937 GACCCTCGTC
SacI 1289 GAGCTC GAGCT^C
1969 GAGCTC
ScaI 1233 AGTACT AGT^ACT
2602 AGTACT
SfcI 365 CTGTAG C^T[GA][CT]AG
1904 CTATAG
SfoI 101 GGCGCC GGC^GCC
947 GGCGCC
SmaI 80 CCCGGG CCC^GGG
695 CCCGGG
StyI 607 CCTAGG C^C[AT][AT]GG
1275 CCTTGG
Tru9I 2015 TTAA T^TAA
2236 TTAA
Tth111I 566 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2317 GACACTGTC
XmaCI 80 CCCGGG C^CCGGG
695 CCCGGG
XmaI 80 CCCGGG C^CCGGG
695 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII