Restriction map for
pF1KA0397
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# NO CUT ENZYMES ( 74 enzymes) #
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AccI AclI AcsI AflII AhdI
Aor51HI ApoI AscI AseI AsnI
AspEI AvrII BciVI BfrI BglII
BlnI BsaBI BsaI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstXI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI HincII HindII
HindIII HpaI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI SexAI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1805 GGTACC G^GTACC
AgeI 1439 ACCGGT A^CCGGT
Alw44I 1561 GTGCAC G^TGCAC
ApaLI 1561 GTGCAC G^TGCAC
Asp700I 1164 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1805 GGTACC G^GTACC
AviII 188 TGCGCA TGC^GCA
BalI 1740 TGGCCA TGG^CCA
BbeI 2529 GGCGCC GGCGC^C
BbvCI 363 CCTCAGC CCTCAGC(-5/-2)
BcgI 1904 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2556 TGATCA T^GATCA
BsiEI 1437 CGACCG CG[GA][CT]^CG
BsrDI 120 CATTGC GCAATG(2/0)
BstAPI 980 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2191 TTCGAA TT^CGAA
BstEII 2541 GGTCACC G^GT[ACGT]ACC
BstPI 2541 GGTCACC G^GT[ACGT]ACC
DraIII 2989 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1542 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2541 GGTCACC G^GT[ACGT]ACC
EcoT22I 2695 ATGCAT ATGCA^T
FbaI 2556 TGATCA T^GATCA
FspI 188 TGCGCA TGC^GCA
KasI 2529 GGCGCC G^GCGCC
KpnI 1805 GGTACC GGTAC^C
KspI 1604 CCGCGG CCGC^GG
MluNI 1740 TGGCCA TGG^CCA
MscI 1740 TGGCCA TGG^CCA
NarI 2529 GGCGCC GG^CGCC
NcoI 2627 CCATGG C^CATGG
NsiI 2695 ATGCAT ATGCA^T
NspV 2191 TTCGAA TT^CGAA
PaeR7I 2446 CTCGAG C^TCGAG
PinAI 1439 ACCGGT A^CCGGT
SacII 1604 CCGCGG CCGC^GG
ScaI 461 AGTACT AGT^ACT
SfoI 2529 GGCGCC GGC^GCC
SfuI 2191 TTCGAA TT^CGAA
TfiI 2017 GATTC G^A[AT]TC
XcmI 888 CCAGCTCATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2446 CTCGAG C^TCGAG
XmnI 1164 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AatII 1720 GACGTC GACGT^C
2461 GACGTC
AccIII 674 TCCGGA T^CCGGA
1253 TCCGGA
ApaI 1321 GGGCCC GGGCC^C
2230 GGGCCC
BamHI 671 GGATCC G^GATCC
2268 GGATCC
BbrPI 790 CACGTG CAC^GTG
1921 CACGTG
BbsI 103 GAAGAC GAAGAC(2/6)
228 GAAGAC
BlpI 338 GCTCAGC GC^T[ACGT]AGC
2938 GCTGAGC
Bpu1102I 338 GCTCAGC GC^T[ACGT]AGC
2938 GCTGAGC
BpuAI 103 GAAGAC GAAGAC(2/6)
228 GAAGAC
BseAI 674 TCCGGA T^CCGGA
1253 TCCGGA
BsmBI 165 GAGACG CGTCTC(1/5)
2459 GAGACG
BsmI 1196 GCATTC GAATGC(1/-1)
2085 GCATTC
BspEI 674 TCCGGA T^CCGGA
1253 TCCGGA
BspHI 893 TCATGA T^CATGA
2588 TCATGA
BsrFI 1439 ACCGGT [GA]^CCGG[CT]
1586 ACCGGC
CelII 338 GCTCAGC GC^T[ACGT]AGC
2938 GCTGAGC
Cfr10I 1439 ACCGGT [GA]^CCGG[CT]
1586 ACCGGC
EaeI 1269 CGGCCA [CT]^GGCC[GA]
1740 TGGCCA
MroI 674 TCCGGA T^CCGGA
1253 TCCGGA
MseI 2374 TTAA T^TAA
2756 TTAA
PflMI 789 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2664 CCAGATCCTGG
PmaCI 790 CACGTG CAC^GTG
1921 CACGTG
PmlI 790 CACGTG CAC^GTG
1921 CACGTG
RcaI 893 TCATGA T^CATGA
2588 TCATGA
Tru9I 2374 TTAA T^TAA
2756 TTAA
Tsp509I 2134 AATT ^AATT
2354 AATT
Van91I 789 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2664 CCAGATCCTGG
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AspI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I XhoII XmaCI XmaI