################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AccIII AclI AflII AhdI AscI AseI AsnI Asp700I AspEI AspI AviII BfrI BseAI BsiWI BspEI BssHII Bst1107I BstZ17I CpoI Eam1105I EcoRV EcoT22I FseI FspI HpaI MfeI MluI MroI MunI NotI NruI NsiI PacI PflFI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 4675 GACGTC GACGT^C Acc65I 2102 GGTACC G^GTACC AccI 6454 GTAGAC GT^[AC][GT]AC AgeI 5931 ACCGGT A^CCGGT Asp718I 2102 GGTACC G^GTACC AvrII 5430 CCTAGG C^CTAGG BbeI 322 GGCGCC GGCGC^C BbvCI 373 GCTGAGG CCTCAGC(-5/-2) BlnI 5430 CCTAGG C^CTAGG BsaBI 2769 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 8384 CGGCCG CG[GA][CT]^CG Bsp1407I 2492 TGTACA T^GTACA BspDI 5378 ATCGAT AT^CGAT BsrBI 256 GAGCGG CCGCTC(-3/-3) BsrGI 2492 TGTACA T^GTACA BssSI 6736 CTCGTG CACGAG(-5/-1) BstBI 4142 TTCGAA TT^CGAA BstEII 5637 GGTAACC G^GT[ACGT]ACC BstPI 5637 GGTAACC G^GT[ACGT]ACC ClaI 5378 ATCGAT AT^CGAT DraI 867 TTTAAA TTT^AAA DrdI 2800 GACACTCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 8384 CGGCCG C^GGCCG EclXI 8384 CGGCCG C^GGCCG Eco52I 8384 CGGCCG C^GGCCG EcoO65I 5637 GGTAACC G^GT[ACGT]ACC HindIII 1879 AAGCTT A^AGCTT KasI 322 GGCGCC G^GCGCC KpnI 2102 GGTACC GGTAC^C MamI 2769 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 322 GGCGCC GG^CGCC NdeI 7942 CATATG CA^TATG NheI 6142 GCTAGC G^CTAGC NspV 4142 TTCGAA TT^CGAA PinAI 5931 ACCGGT A^CCGGT ScaI 6962 AGTACT AGT^ACT SexAI 2237 ACCTGGT A^CC[AT]GGT SfoI 322 GGCGCC GGC^GCC SfuI 4142 TTCGAA TT^CGAA SmaI 108 CCCGGG CCC^GGG SspBI 2492 TGTACA T^GTACA SspI 2024 AATATT AAT^ATT XmaCI 108 CCCGGG C^CCGGG XmaI 108 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### Aor51HI 126 AGCGCT AGC^GCT 2069 AGCGCT BamHI 2721 GGATCC G^GATCC 6099 GGATCC BbrPI 7861 CACGTG CAC^GTG 7897 CACGTG BsmI 969 GAATGC GAATGC(1/-1) 2353 GAATGC Eco47III 126 AGCGCT AGC^GCT 2069 AGCGCT PaeR7I 2822 CTCGAG C^TCGAG 5907 CTCGAG PmaCI 7861 CACGTG CAC^GTG 7897 CACGTG PmlI 7861 CACGTG CAC^GTG 7897 CACGTG PshAI 3532 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4456 GACCAGAGTC SphI 5816 GCATGC GCATG^C 8062 GCATGC XcmI 67 CCACACCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2021 CCAAATATTTGATGG XhoI 2822 CTCGAG C^TCGAG 5907 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 152 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII