Restriction map for
pF1KA0551
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AclI AflII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BsaAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII BstBI BstEII BstPI
Bsu36I ClaI CpoI DraI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HindIII HpaI
KasI KpnI KspI MluI NarI
NcoI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Alw44I 1831 GTGCAC G^TGCAC
ApaLI 1831 GTGCAC G^TGCAC
BamHI 2257 GGATCC G^GATCC
BbsI 1996 GAAGAC GAAGAC(2/6)
BlpI 526 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC
BpuAI 1996 GAAGAC GAAGAC(2/6)
BsaBI 2401 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 4046 TCATGA T^CATGA
BspLU11I 212 ACATGT A^CATGT
BsrDI 2759 GCAATG GCAATG(2/0)
BssSI 1834 CACGAG CACGAG(-5/-1)
CelII 526 GCTCAGC GC^T[ACGT]AGC
DraIII 1891 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 511 GACTTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2162 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2058 GAATTC G^AATTC
EcoT22I 3949 ATGCAT ATGCA^T
FauI 1131 GCGGG CCCGC(4/6)
MamI 2401 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1271 GCCGGC GCC^GGC
NdeI 626 CATATG CA^TATG
NgoMIV 1271 GCCGGC G^CCGGC
NheI 2380 GCTAGC G^CTAGC
NsiI 3949 ATGCAT ATGCA^T
RcaI 4046 TCATGA T^CATGA
SbfI 61 CCTGCAGG CCTGCA^GG
SexAI 2792 ACCTGGT A^CC[AT]GGT
SmlI 904 CTCAAG C^T[CT][GA]AG
SpeI 504 ACTAGT A^CTAGT
Sse8387I 61 CCTGCAGG CCTGCA^GG
StuI 1531 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 36 enzymes) #
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AatII 2721 GACGTC GACGT^C
2949 GACGTC
AccII 1894 CGCG CG^CG
2845 CGCG
AccIII 2132 TCCGGA T^CCGGA
2441 TCCGGA
AcyI 2721 GACGTC G[GA]^CG[CT]C
2949 GACGTC
AflIII 212 ACATGT A^C[GA][CT]GT
368 ACACGT
AhdI 643 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3287 GACTGAATGTC
AspEI 643 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3287 GACTGAATGTC
BanI 585 GGCACC G^G[CT][GA]CC
2013 GGTGCC
BclI 347 TGATCA T^GATCA
3245 TGATCA
BsaHI 2721 GACGTC G[GA]^CG[CT]C
2949 GACGTC
BseAI 2132 TCCGGA T^CCGGA
2441 TCCGGA
BsmI 3489 GAATGC GAATGC(1/-1)
896 GCATTC
BspEI 2132 TCCGGA T^CCGGA
2441 TCCGGA
BsrFI 1271 GCCGGC [GA]^CCGG[CT]
1631 ACCGGC
Bst1107I 2941 GTATAC GTA^TAC
3431 GTATAC
BstAPI 679 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1389 GCAGCTACTGC
BstUI 1894 CGCG CG^CG
2845 CGCG
BstXI 668 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3848 CCACGTCTGTGG
BstZ17I 2941 GTATAC GTA^TAC
3431 GTATAC
Cfr10I 1271 GCCGGC [GA]^CCGG[CT]
1631 ACCGGC
Eam1105I 643 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3287 GACTGAATGTC
EcoRV 1674 GATATC GAT^ATC
3679 GATATC
FbaI 347 TGATCA T^GATCA
3245 TGATCA
HaeII 747 AGCGCC [GA]GCGC^[CT]
1268 AGCGCC
Hin1I 2721 GACGTC G[GA]^CG[CT]C
2949 GACGTC
HincII 1041 GTCGAC GT[CT]^[GA]AC
2372 GTCGAC
HindII 1041 GTCGAC GT[CT]^[GA]AC
2372 GTCGAC
MfeI 844 CAATTG C^AATTG
1435 CAATTG
MroI 2132 TCCGGA T^CCGGA
2441 TCCGGA
MunI 844 CAATTG C^AATTG
1435 CAATTG
MvnI 1894 CGCG CG^CG
2845 CGCG
NspI 212 ACATGT [GA]CATG^[CT]
704 ACATGC
PflMI 1890 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCAGGAGATGG
SalI 1041 GTCGAC G^TCGAC
2372 GTCGAC
SspI 224 AATATT AAT^ATT
241 AATATT
Van91I 1890 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCAGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI