Restriction map for
pF1KA0556
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# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AviII AvrII BbeI BbrPI BlnI
BsiWI BsmI BspDI BspHI BssHII
Bst1107I BstAPI BstZ17I Bsu36I ClaI
CpoI Eco81I EcoNI FseI FspI
HpaI KasI KpnI MluI NarI
NheI NotI NruI PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SfoI SgrAI
SnaBI SpeI SphI SwaI XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 4360 GACGTC GACGT^C
AccI 2946 GTCTAC GT^[AC][GT]AC
AflII 351 CTTAAG C^TTAAG
AgeI 3012 ACCGGT A^CCGGT
Aor51HI 2924 AGCGCT AGC^GCT
Asp700I 2042 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 4843 CGACGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3245 GGATAC GTATCC(6/5)
BfrI 351 CTTAAG C^TTAAG
BglI 4250 GCCTCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3740 AGATCT A^GATCT
BlpI 4105 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4105 GCTCAGC GC^T[ACGT]AGC
BsaAI 1432 TACGTG [CT]AC^GT[GA]
BsaBI 1738 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4574 TGTACA T^GTACA
BsrDI 3472 GCAATG GCAATG(2/0)
BsrGI 4574 TGTACA T^GTACA
BstBI 1670 TTCGAA TT^CGAA
BstEII 3090 GGTCACC G^GT[ACGT]ACC
BstPI 3090 GGTCACC G^GT[ACGT]ACC
CelII 4105 GCTCAGC GC^T[ACGT]AGC
DraI 2896 TTTAAA TTT^AAA
Eco47III 2924 AGCGCT AGC^GCT
EcoO65I 3090 GGTCACC G^GT[ACGT]ACC
EcoRI 2046 GAATTC G^AATTC
EcoT22I 3133 ATGCAT ATGCA^T
KspI 250 CCGCGG CCGC^GG
MamI 1738 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 175 CAATTG C^AATTG
MunI 175 CAATTG C^AATTG
NdeI 3847 CATATG CA^TATG
NsiI 3133 ATGCAT ATGCA^T
NspV 1670 TTCGAA TT^CGAA
PaeR7I 3397 CTCGAG C^TCGAG
PinAI 3012 ACCGGT A^CCGGT
PshAI 962 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 250 CCGCGG CCGC^GG
SbfI 3456 CCTGCAGG CCTGCA^GG
ScaI 3779 AGTACT AGT^ACT
SfiI 4249 GGCCTCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1670 TTCGAA TT^CGAA
Sse8387I 3456 CCTGCAGG CCTGCA^GG
SspBI 4574 TGTACA T^GTACA
TfiI 3626 GAATC G^A[AT]TC
XhoI 3397 CTCGAG C^TCGAG
XmnI 2042 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 1550 TCCGGA T^CCGGA
4037 TCCGGA
AcyI 2643 GACGCC G[GA]^CG[CT]C
4360 GACGTC
AflIII 4418 ACATGT A^C[GA][CT]GT
4627 ACATGT
AspI 2371 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2643 GACGCCGTC
BalI 1703 TGGCCA TGG^CCA
4589 TGGCCA
BamHI 3551 GGATCC G^GATCC
4583 GGATCC
BbvCI 1074 CCTCAGC CCTCAGC(-5/-2)
853 GCTGAGG
BclI 3215 TGATCA T^GATCA
4496 TGATCA
BsaHI 2643 GACGCC G[GA]^CG[CT]C
4360 GACGTC
BseAI 1550 TCCGGA T^CCGGA
4037 TCCGGA
BspEI 1550 TCCGGA T^CCGGA
4037 TCCGGA
BspLU11I 4418 ACATGT A^CATGT
4627 ACATGT
BsrBI 2721 CCGCTC CCGCTC(-3/-3)
3511 GAGCGG
DrdI 3130 GACATGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3880 GACCATGTGGTC
EagI 1217 CGGCCG C^GGCCG
3491 CGGCCG
EclXI 1217 CGGCCG C^GGCCG
3491 CGGCCG
Eco52I 1217 CGGCCG C^GGCCG
3491 CGGCCG
EcoRV 1546 GATATC GAT^ATC
3049 GATATC
FbaI 3215 TGATCA T^GATCA
4496 TGATCA
Hin1I 2643 GACGCC G[GA]^CG[CT]C
4360 GACGTC
MluNI 1703 TGGCCA TGG^CCA
4589 TGGCCA
MroI 1550 TCCGGA T^CCGGA
4037 TCCGGA
MscI 1703 TGGCCA TGG^CCA
4589 TGGCCA
PflFI 2371 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2643 GACGCCGTC
SapI 1872 GAAGAGC GCTCTTC(1/4)
752 GCTCTTC
SexAI 2629 ACCTGGT A^CC[AT]GGT
4604 ACCTGGT
SmaI 2242 CCCGGG CCC^GGG
2757 CCCGGG
Tth111I 2371 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2643 GACGCCGTC
XmaCI 2242 CCCGGG C^CCGGG
2757 CCCGGG
XmaI 2242 CCCGGG C^CCGGG
2757 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AspEI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII